Microbial and soil properties in restoration areas in the jequitinhonha valley, Minas Gerais

To mitigate the impacts of eucalypt monoculture, forestry companies in the Upper Jequitinhonha Valley (MG) have adopted the insertion of strips of native vegetation in-between the commercial plantations. The method used for the creation of these corridors is to allow spontaneous regrowth of native vegetation in areas previously under eucalypt. The objective of this study was to evaluate the effect of cover crops on microbial and soil properties for a detailed description of the restoration process of native vegetation in forest soils of the Jequitinhonha Valley. The treatments were represented by an initial restoration stage (< 4 years) with or without remaining eucalypt and the advanced restoration stage (> 4 years) with or without remaining eucalypt, plus the three controls: commercial eucalypt plantation, Cerrado vegetation and native forest. Soil samples were collected for three consecutive years in the dry and rainy season (August and February, respectively). The microbial activity, regardless of the presence of remaining eucalypt , did not differ among the restoration areas, except for the metabolic quotient (qCO2) in the rainy season of February 2007. At this time, this microbial activity was higher in the advanced restoration stage without eucalypt than initial restoration without eucalypt and advanced restoration with eucalypt. The restoration areas, in general, did not differ from the control: eucalypt plantation and Cerrado either. Compared to the forest, the levels of organic C, microbial C, basal respiration (Rbasal) and hydrolysis of fluorescein diacetate (FDA) in the restoration areas were, in general, lower and did not differ in qCO2 and microbial quotient (qMIC). In general, the soil quality was similar in the initial and advanced restoration stages. Most of the soil and microbial properties in the three years indicated that the restoration areas were most similar to the Cerrado. In the advanced restoration areas without eucalypt compared to Cerrado, the lower Rbasal in the 3rd year and the lower FDA and qMIC and higher qCO2 in the 2nd year indicated that the removal of the remaining eucalypt trees was unfavorable for restoration.

Strategy of specification of management areas: rice grain yield as related to soil fertility

It is well-known nowadays that soil variability can influence crop yields. Therefore, to determine specific areas of soil management, we studied the Pearson and spatial correlations of rice grain yield with organic matter content and pH of an Oxisol (Typic Acrustox) under no- tillage, in the 2009/10 growing season, in Selvíria, State of Mato Grosso do Sul, in the Brazilian Cerrado (longitude 51º24' 21'' W, latitude 20º20' 56'' S). The upland rice cultivar IAC 202 was used as test plant. A geostatistical grid was installed for soil and plant data collection, with 120 sampling points in an area of 3.0 ha with a homogeneous slope of 0.055 m m-1. The properties rice grain yield and organic matter content, pH and potential acidity and aluminum content were analyzed in the 0-0.10 and 0.10-0.20 m soil layers. Spatially, two specific areas of agricultural land management were discriminated, differing in the value of organic matter and rice grain yield, respectively with fertilization at variable rates in the second zone, a substantial increase in agricultural productivity can be obtained. The organic matter content was confirmed as a good indicator of soil quality, when spatially correlated with rice grain yield.

Methane fluxes from waterlogged and drained Histosols of highland areas

Soil can be either source or sink of methane (CH4), depending on the balance between methanogenesis and methanotrophy, which are determined by pedological, climatic and management factors. The objective of this study was to assess the impact of drainage of a highland Haplic Histosol on CH4 fluxes. Field research was carried out in Ponta Grossa (Paraná, Brazil) based on the measurement of CH4 fluxes by the static chamber method in natural and drained Histosol, over one year (17 sampling events). The natural Histosol showed net CH4 eflux, with rates varying from 238 µg m-2 h-1 CH4, in cool/cold periods, to 2,850 µg m-2 h-1 CH4, in warm/hot periods, resulting a cumulative emission of 116 kg ha-1 yr-1 CH4. In the opposite, the drained Histosol showed net influx of CH4 (-39 to -146 µg m-2 h-1), which resulted in a net consumption of 9 kg ha-1 yr-1 CH4. The main driving factors of CH4 consumption in the drained soil were the lowering of the water-table (on average -57 cm, vs -7 cm in natural soil) and the lower water content in the 0-10 cm layer (average of 5.5 kg kg-1, vs 9.9 kg kg-1 in natural soil). Although waterlogged Histosols of highland areas are regarded as CH4 sources, they fulfill fundamental functions in the ecosystem, such as the accumulation of organic carbon (581 Mg ha-1 C to a depth of 1 m) and water (8.6 million L ha-1 = 860 mm to a depth of 1 m). For this reason, these soils must not be drained as an alternative to mitigate CH4 emission, but effectively preserved.

Variability of soil fertility properties in areas planted to sugarcane in the State of Goias, Brazil

Soil sampling should provide an accurate representation of a given area so that recommendations for amendments of soil acidity, fertilization and soil conservation may be drafted to increase yield and improve the use of inputs. The aim of this study was to evaluate the variability of soil fertility properties of Oxisols in areas planted to sugarcane in the State of Goias, Brazil. Two areas of approximately 8,100 m² each were selected, representing two fields of the Goiasa sugarcane mill in Goiatuba. The sugarcane crop had a row spacing of 1.5 m and subsamples were taken from 49 points in the row and 49 between the row with a Dutch auger at depths of 0.0-0.2 and 0.2-0.4 m, for a total of 196 subsamples for each area. The samples were individually subjected to chemical analyses of soil fertility (pH in CaCl2, potential acidity, organic matter, P, K, Ca and Mg) and particle size analysis. The number of subsamples required to compose a sample within the acceptable ranges of error of 5, 10, 20 and 40 % of each property were computed from the coefficients of variation and the Student t-value for 95 % confidence. The soil properties under analysis exhibited different variabilities: high (P and K), medium (potential acidity, Ca and Mg) and low (pH, organic matter and clay content). Most of the properties analyzed showed an error of less than 20 % for a group of 20 subsamples, except for P and K, which were capable of showing an error greater than 40 % around the mean. The extreme variability in phosphorus, particularly at the depth of 0.2-0.4 m, attributed to banded application of high rates of P fertilizers at planting, places limitations on assessment of its availability due to the high number of subsamples required for a composite sample.

Use of legal reserve areas as geochemical background in hydrosedimentology studies¹

In hydrosedimentology studies the determination of the trace element concentrations at the study site is imperative, since this background can be used to assess the enrichment of sediments with these elements. This enrichment can be the result of the natural process of geological formation or of anthropogenic activities. In the latter case, guidelines are used to indicate the concentrations at which trace elements cause ecotoxicity effects on the environment. Thus, this study used legal reserve areas in the municipality of Toledo, PR, where natural forests are maintained, with no or minimal human interference to establish background levels. The results of atomic emission spectrometry with inductively coupled argon plasma showed that the legal reserves have lower levels of trace elements than other theoretical references, but equivalent concentrations to the safety levels recommended by international guidelines. It was concluded that determining values is fundamental to recommend this background as scientific database for research in the area of hydrosedimentology of this site and also as a way of environmental management of the watershed of this municipality.

Rhizobia Isolated from Coal Mining Areas in the Nodulation and Growth of Leguminous Trees

ABSTRACT An alternative for recovery of areas degraded by coal mining is revegetation with rapidly growing leguminous trees, which often do not establish in low fertility soils. The objective of this study was to evaluate the efficiency of native rhizobia isolated from coal mining areas in the nodulation and growth of leguminous trees. We isolated 19 strains of rhizobia from a degraded soil near Criciúma, SC, Brazil, and evaluated the nodulation and growth-promoting capacity of the inoculated isolates for bracatinga (Mimosa scabrella), maricá (M. bimucronata) and angico-vermelho (Parapiptadenia rigida). Isolates UFSC-B2, B6, B8, B9, B11 and B16 were able to nodulate bracatinga, providing average increases of 165 % in shoot dry matter, with a significant contribution to N accumulation. Isolates UFSC-B5, B12, and M8 favored nodulation and growth of maricá, especially isolate UFSC-B12, which promoted increases of 370 % in N accumulation compared to treatment with N fertilizer. All strains were inefficient in promoting growth and N uptake by angico-vermelho. In conclusion, isolation and use of selected rhizobia for bracatinga and maricá plant inoculation can contribute to the growth and accumulation of N, with prospects for use in programs for revegetation of degraded soils in coal mining areas.

Provenance and family variation of Pinus caribaea var. hondurensis from Guatemala and Honduras, grown in Brazil, Colombia and Venezuela

Pinus caribaea var. hondurensis (Sénécl) Barr. & Golf. is a tropical pine that naturally occurs in lowland areas of Belize, El Salvador, Guatemala, Honduras, Nicaragua, and eastern Mexico. It has been one of the most studied tropical pines and the one with the most commercial importance in Brazil. The objective of this work was to select the best provenances for plantations and best trees in families for the establishment of seed orchards. For that a trial with five provenances and 47 open-pollinated families was planted near Planaltina, Federal District, in the Cerrado Region of Brazil. The provenances tested were Poptun (Guatemala), Gualjoco, Los Limones, El Porvenir and Santa Cruz de Yojoa (Honduras) and assessed at 12 years of age. Poptun and Gualjoco had larger volume, and Los Limones and El Porvenir the lowest incidence of forks and foxtails. Individual tree heritabilities for volume, stem form and branch diameter were 0.34, 0.06, and 0.26 respectively. More than 90% of the trees had defects, common in unimproved P. caribaea. Selection criteria for quality traits need to be relaxed in the first generation of breeding to allow for larger genetic gains in productivity. Results from this test compared with P. caribaea var. hondurensis trials in other Brazilian, Colombian and Venezuelan sites suggest that provenance x site and family x site interactions are not as strong as in other pine species.

Colonization by mites of glacier-free areas in King George Island, Antarctica

This work aimed to investigate the ratio of colonization by terrestrial mites on ice-free areas created by the ongoing climate-induced melting of Antarctic glaciers. Glacier retreat opens new ice-free areas for the colonization by vegetation and animals. The study was undertaken on the Antarctic Specially Protected Area no. 128 (West Coast of the Admiralty Bay, King George Island, South Shetlands Islands). Transects marked between the Ecology, Baranowski and Windy Glaciers, and a sea shore were used to collect soil samples. Oribatid mites were found only on near-shore areas, on patches of vegetation of more than 30 years of age. The colonization by mite communities is strongly determined by the presence of plants.

Composition and functional groups of epiedaphic ants (Hymenoptera: Formicidae) in irrigated agroecosystem and in nonagricultural areas

The objective of this work was to evaluate the species composition and functional groups of ants in nonagricultural (NA) and in irrigated areas (S, seasonal irrigation; P, irrigation with well water; W, irrigation with wastewater) in an arid agricultural region in central Mexico, throughout 2005 and 2006. A total of 52,358 ants belonging to 6 subfamilies, 21 genera and 39 species was collected using pitfall traps. The species best represented in all plots were: Forelius pruinosus, Pheidole obtusospinosa, Monomorium minimum and Dorymyrmex spp. NA plots recorded the highest density of ants. The highest values for diversity (H') and equitativity (J') were recorded in NA and P plots, while the lowest were recorded in W plots. Cluster analysis showed two different groups regarding species composition: NA-S and W-P. Functional groups recorded were: dominant Dolichoderinae, three species; subordinate Camponotini, five species; hot climate specialists, three species; tropical climate specialists, seven species; cold climate specialists, five species; cryptic species, one species; opportunists, six species; generalized Myrmicinae, nine species. Agricultural activity affects the structure of the ant community with epiedaphic forage, and the constant use of irrigation wastewater in conjunction with intense agricultural practices has negative effect upon species richness of epiedaphic ants.

Estimating soybean crop areas using spectral-temporal surfaces derived from MODIS images in Mato Grosso, Brazil

The objective of this work was to evaluate the application of the spectral-temporal response surface (STRS) classification method on Moderate Resolution Imaging Spectroradiometer (MODIS, 250 m) sensor images in order to estimate soybean areas in Mato Grosso state, Brazil. The classification was carried out using the maximum likelihood algorithm (MLA) adapted to the STRS method. Thirty segments of 30x30 km were chosen along the main agricultural regions of Mato Grosso state, using data from the summer season of 2005/2006 (from October to March), and were mapped based on fieldwork data, TM/Landsat-5 and CCD/CBERS-2 images. Five thematic classes were considered: Soybean, Forest, Cerrado, Pasture and Bare Soil. The classification by the STRS method was done over an area intersected with a subset of 30x30-km segments. In regions with soybean predominance, STRS classification overestimated in 21.31% of the reference values. In regions where soybean fields were less prevalent, the classifier overestimated 132.37% in the acreage of the reference. The overall classification accuracy was 80%. MODIS sensor images and the STRS algorithm showed to be promising for the classification of soybean areas in regions with the predominance of large farms. However, the results for fragmented areas and smaller farms were less efficient, overestimating soybean areas.

Novel santalane sesquiterpenoids from the stem bark of Duguetia glabriuscula - Annonaceae

Five novel santalane-type sesquiterpenes were isolated from the stem bark of Duguetia glabriuscula - Annonaceae. Their structures have been established on the basis of spectral data and chemical evidences (¹H and 13C NMR, HMQC, HMBC) as (+)-alpha-santal-10-en-9-ol (1), (+)-alpha-santalan-10,11-epoxy-9-ol (2), alpha-santal-11-en-9,10-diol (3), (+)-alpha-santalan-9,10,11-triol (4), and (+)-alpha-santalan-9,11-epoxy-10-ol (5). Polycarpol, a triterpenoid, was also obtained.

Air pollution and emergency admissions for cardiorespiratory diseases in Lisbon (Portugal)

Daily records of hospital admissions due to cardiorespiratory diseases and levels of PM10, SO2, CO, NO, NO2, and O3 were collected from 1999-2004 to evaluate the relationship between air pollution and morbidity in Lisbon. Generalised additive Poisson regression models were adopted, controlling for temperature, humidity, and both short and long-term seasonality. Significant positive associations, lagged by 1 or 2 days, were found between markers of traffic-related pollution (CO and NO2) and cardiocirculatory diseases in all age groups. Increased childhood emergency admissions for respiratory illness were significantly correlated with the 1-day lagged SO2 levels coming from industrial activities.

Bioaccumulation of metals in aquatic insects of streams located in areas with sugar cane cultivation

Streams located in areas of sugar cane cultivation receive elevated concentrations of metal ions from soils of adjacent areas. The accumulation of metals in the sediments results in environmental problems and leads to bioaccumulation of metal ions by the aquatic organisms. In the present study, bioaccumulation of the metals ions Al, Cd, Cr, Cu, Fe, Mg, Mn and Zn in aquatic insects in streams impacted by the sugar cane was evaluated. The results pointed out that the insects were contaminated by the sediment and that the collector organisms as Chironomus species accumulated higher concentration of metals than the predator organisms.

Ent-kaurane diterpenoids and other constituents from the stem of Xylopia laevigata (Annonaceae)

Phytochemical investigation of the hexane extract from the stem of Xylopia laevigata led to the isolation of the ent-kaurane diterpenoids, ent-kaur-16-en-19-oic acid, 4-epi-kaurenic acid, ent-16β-hydroxy-17-acetoxy-kauran-19-al, ent-3β-hydroxy-kaur-16-en-19-oic acid, and ent-16β,17-dihydroxy-kauran-19-oic acid, as well as spathulenol and a mixture of β-sitosterol, stigmasterol and campesterol. The identification of the compounds was performed on the basis of spectrometric methods including GC-MS, IR, and 1D and 2D NMR. Potent larvicidal activity against Aedes aegypti larvae with LC50 of 62.7 µg mL-1 was found for ent-3β-hydroxy-kaur-16-en-19-oic acid. This compound also showed significant antifungal activity against Candida glabrata and Candida dubliniensis with MIC values of 62.5 µg mL-1.