Photosynthetic activity of cassava plants under weed competition

The objective of this work was to evaluate characteristics associated with the photosynthetic activity of cassava plants under weed competition. The trial was carried out under field conditions, and experimental units consisted of 150 dm³ fiberglass boxes containing red yellow Latosol, previously corrected and fertilized. Treatments consisted in the cultivation of cassava plants which were free of weed competition and associated with three weed species: Bidens pilosa, Commelina benghalensis or Brachiaria plantaginea. After manioc sprouting started, 15 days after being planted, weeds that had been sown when manioc was planted were thinned, there were then eight plants left per experimental unit in accordance with specified treatments: cassava free of competition, cassava competing with B. pilosa, cassava competing with C. benghalensis and cassava competing with B. plantaginea. Sixty days after crop emergence leaf internal CO2 concentration (Ci), leaf temperature at the time of evaluation (Tleaf) and photosynthetic rate (A) were evaluated, also the CO2 consumption rate (ΔC) of cassava plants was calculated. A correlation matrix between variables was also obtained. All characteristics associated with photosynthesis in cassava plants were influenced by weed species. Cassava was more affected by B. pilosa and B. plantaginea in which concerns its exposition to solar radiation and water, while C. benghalensis seems to mostly affect the composition of incident light on the culture, allowing cassava to anticipate imposition when competing, even before it reaches harmful levels.

Photosynthetic characteristics of hybrid and conventional rice plants as a function of plant competition

The objective of this work was to evaluate the characteristics related to the photosynthetic ability of hybrid and inbred rice varieties, as a way to assess which of the two presented higher potential to stand out under conditions of competition. The trial was set in a greenhouse in completely randomized block design and 2 x 6 factorial scheme with four replications. Factor A consisted of rice varieties (hybrid or inbred) and factor B by competition levels. Treatments consisted in maintaining one plant of either BRS Pelota (inbred) or Inov (hybrid) variety at the center of the plot, under competition with 0, 1, 2, 3, 4 or 5 plants of the variety BRS Pelota at the periphery of the experimental unit, according to the treatment. Fifty days after emergence (DAE), sub-stomatal CO2 concentration (Ci - mmol mol-1), photosynthetic rate (A - mmol m-2 s-1) and CO2 consumed (DC - mmol mol-1) were quantified, as well as shoot dry mass(SDM).Hybrid plants present higher photosynthesis capacity than inbred plants, when competing with up to 3 times its own density. When under the same competitive intensity, hybrid plants surpass the inbred. However, it should be emphasized that, when in farm condition, the lower competitive capacity with weeds often attributed to the hybrid varieties, probably is due to their lower planting density, but if weed competition is kept at low levels, hybrid rice plants may perform in the same way or usually better than inbred plants.

Efficacy of herbicides applied to Digitaria horizontalis plants under different water conditions

This project aimed to relate the control efficiency of ACCase inhibiting herbicides applied post-emergence to Digitaria horizontalis plants under different soil water contents. The experiments were conducted in a greenhouse, with the application of three different herbicides (fluazifop-p-butyl, haloxyfop-methyl, and sethoxydim + mineral oil Assist). The experimental design used for each herbicide was completely randomized, with four replications, consisting of a 3 x 4 factorial, with the combination of water management strategies (-0.03, -0.07 and -1.5 MPa) and four doses of these products (100%, 50%, 25%, and 0% of the recommended dose). Herbicide application was made at two vegetative stages, 4-6 leaves and 2-3 tillers. The visual phytotoxicity evaluations were performed at 14 days after application and the plant dry weight at the end of the study was evaluated. The control efficiency was not affected by water management strategies when applied to the recommended dose of the herbicides in early stages of plant development (4-6 leaf stage). In late applications (2-3 tiller stage) the plants held under drought stress showed less phytotoxicity.

Sourgrass densities affecting the initial growth and macronutrient content of coffee plants

The objective of this work was to evaluate the coexistence effects of coffee (Coffea arabica) with densities of sourgrass (Digitaria insularis) on crop macronutrient content and plant growth. The experiment was conducted in plots where one coffee plant was maintained in coexistence with 0 (weed-free check), 1, 2, 4, 8, and 16 sourgrass plants, using a completely randomized design with three replicates. Reduction of coffee growth and macronutrient content, except P that increased, started when the coexistence occurred with sourgrass plants in a density of 1 plant per plot. In general, macronutrient content was reduced by 18-50%, while growth characteristics were reduced by 9-41%, when coffee plants coexisted with 16 plants of sourgrass. Thus, sourgrass competition for nutrients was a strong factor limiting coffee plant growth.

Glyphosate translocation in herbicide tolerant plants

The objective of this study was to evaluate glyphosate translocation in glyphosate-tolerant weed species (I. nil, T. procumbens and S. latifolia) compared to glyphosate-susceptible species (B. pilosa). The evaluations of 14C-glyphosate absorption and translocation were performed at 6, 12, 36 and 72 hours after treatment (HAT) in I. nil and B. pilosa, and only at 72 HAT in the species T. procumbens and S. latifolia. The plants were collected and fractionated into application leaf, other leaves, stems, and roots. In S. latifolia, approximately 88% of the glyphosate remained in the application leaf and a small amount was translocated to roots at 72 HAT. However, 75% of the herbicide applied on T. procumbens remained in the leaf that had received the treatment, with greater glyphosate translocation to the floral bud. It was concluded that the smaller amount of glyphosate observed in S. latifolia and T. procumbens may partly account for their higher tolerance to glyphosate. However, I. nil tolerance to glyphosate may be associated with other factors such as metabolization, root exudation or compartmentalization, because a large amount of the herbicide reached the roots of this species.

Accumulation of dry mass and macronutrients by sourgrass plants

The experiment was carried out aiming to analyze the dry mass production and distribution and the content and accumulation of macronutrients in sourgrass (Digitaria insularis) plants cultivated under mineral nutrition standard conditions. Plants grew in 7-liter pots filled with sand substrate and daily irrigated with nutrient solution, being maintained under greenhouse conditions. Treatments consisted of times of evaluation (21, 35, 49, 63, 77, 91, 105, 119, and 133 days after emergence - DAE) and were arranged in a completely randomized design with four replicates. Sourgrass showed small accumulation of dry mass (0.3 g per plant) and macronutrients (3.7 mg of N per plant, 0.4 mg of P per plant, 5.6 mg of K per plant, 0.9 mg of Ca per plant, 0.7 mg of Mg per plant, and 0.3 mg of S per plant) at vegetative growth stage (< 49 DAE). Those accumulations increased mainly after 77 DAE, reaching the maximum theoretical value at 143, 135, 141, 129, 125, 120, and 128 DAE, for dry mass (12.4 g per plant), N (163.2 mg per plant), P (27.1 mg per plant), K (260.5 mg per plant), Ca (47.6 mg per plant), Mg (30.9 mg per plant), and S (13.7 mg per plant), respectively. K and N were found with higher rates and, as a consequence, they were required and accumulated in greater amounts in plant tissues of sourgrass.

Eucalyptus growth in silvopastoral system under different crown dia meters

The aim of this study was to evaluate the effect of different crown diameters on the early growth of eucalyptus intercropped with Brachiaria decumbens in a silvopastoral system. The experiment was conducted in a B. decumbens established pasture, where hybrid eucalyptus urograndis (clone GG100) was planted, spaced 8 x 3 m. A randomized block design was used, with six replicates. Treatments consisted of five crown diameters (0.0, 1.0, 1.5, 2.0, and 3.0 m) surrounding the eucalyptus plants. Five weeding hoes were performed throughout the experiment, according to the different crown diameters, aiming to maintain the eucalyptus plants free from B. decumbens interference. At 90, 180, 270, and 360 DAP, the height and the diameter of the eucalyptus plants were evaluated, and at 360 DAP, surface biomass and leaf area were evaluated. At 90 DAP, it was verified that the non-weeded plants had lower growth, compared to those submitted to crowns. Crown diameters of 2.51 and 2.64 m allowed greater growth in height and diameter at ground level of eucalyptus plants, respectively, in all periods evaluated. Biomass production and leaf area per plant at 360 DAP were also influenced by the different crown diameters. It was concluded that crown diameter around 2 meters provided favorable conditions for early growth of eucalyptus and less involvement in the area occupied by forage.

Directed-spray application of paraquat and diuron in physic nut plants

There is little information about the selectivity of herbicides in physic nut (Jatropha curcas) in Brazil. Therefore, this study aimed to evaluate the selectivity of different doses and mixtures of paraquat and diuron in direted-spray applications in physic nut plants in greenhouse conditions. The study used a randomized block design, with five replicates. The treatments were: paraquat (200 and 600 g ha-1), diuron (1,000 and 2,000 g ha-1), paraquat + diuron (200 + 1,000 g ha-1), paraquat + diuron (200 + 2,000 g ha-1), paraquat + diuron (600 + 1,000 g ha-1), paraquat + diuron (600 + 2,000 g ha-1) and a control (no application). Directed-spray application was performed at 70 days after sowing by the lower third of the plants. The treatments of diuron and paraquat + diuron mixtures affected the growth and photosynthetic activity of physic nut plants, injuries being more pronounced at doses of diuron of 2,000 g ha‑1, while the isolated application of paraquat at doses of 200 and 600 g ha-1 showed good selectivity potential for physic nut plants.

Chlorophyll a fluorescence in rice plants exposed of herbicides of group imidazolinone

The objective of this work was to investigate the injuries caused to the photosynthetic apparatus of three types of rice exposed to application of imidazolinone group herbicides. Two experiments were conducted using herbicides Imazethapyr+imazapic and Imazapyr+imazapic, in a split-plot experimental design, and a 3 x 3 factorial, with six replications. The first factor (A) consisted of the herbicide rates 0, 100 e 200 g ha-1 of Imazethapyr+imazapic and 0, 140 e 280 g ha-1 of Imazapyr+imazapic; factor B consisted of type of rice (cv. Puitá Inta CL, sensitive red rice ecotype and red rice ecotype with suspected herbicide tolerance to Imidazolinone). Chlorophyll a fluorescence parameters were evaluated in plants at 30 days after herbicide application, using a portable fluorometer (HandyPEA, Hanstech). The photosynthetic metabolism of cv. Puitá Inta CL was found to tolerate commercial dosages of both herbicides. High sensitivity to the herbicides was observed for the sensitive red rice ecotype, while the photosynthetic apparatus of red rice ecotype with suspected herbicide tolerance showed high tolerance to both herbicides applied at rates higher than the commercial rate. The application of chemical herbicides of the imidazolinone group on rice plants causes changes in the photosynthetic metabolism of plants, detected by evaluating the emission of transient chlorophyll a fluorescence. This method can be useful in helping detect resistance and/or tolerance of red rice plants to herbicides of the imidazolinone group.

Glyphosate as a tool to produce shikimic acid in plants

Oseltamivir phosphate is a potent viral inhibitor produced from shikimic acid extracted from seeds of Ilicium verum, the most important natural source. With the site of action 5-enolpyruvylshikimate-3-phosphate synthase (EPSP), glyphosate is the only compound capable of inhibiting its activity with the consequent accumulation of shikimic acid in plants. Corn and soybean plants were sprayed with reduced rates of glyphosate (0.0 to 230.4 g a.i. ha¹) and shikimic acid content in the dry mass was determined by HPLC 3, 7 and 10 days after application. Results showed shikimic acid accumulation in dry mass with increases of up to 969% in corn and 33,000% on soybeans, with peak concentrations 3 days after treatment (DAT). Industrial feasibility for shikimic acid production, combined with favorable climatic conditions for growing corn and soybean in virtually all over Brazil, favor the use of reduced rates of glyphosate in shikimic acid biosynthesis, with potential for use as an inducer in exploration of alternative sources for production of oseltamivir phosphate with low environmental impact.

The Allelopathic Effect of Eucalyptus Leaf Extract on Grass Forage Seed

This study aimed to evaluate the allelopathic effect of various concentrations of an aqueous extract of eucalyptus leaves on Urochloa decumbens and Panicum maximum seeds. The extract was prepared from Eucalyptus urograndis leaves that were milled and mixed with distilled water in a 1:9 milled leaves: water ratio to obtain an extract with a defined concentration of 100%. In addition, dilutions of 50%, 25% and 12.5% were prepared, and a 0% dilution was used as a control. The experiment followed a completely randomized design, with four replicates, each of 50 seeds of U. decumbens and 50 seeds of P. maximum, arranged on filter paper moistened with each concentration of extract in a Gerbox plastic box. The results demonstrated the allelopathic potential of E. urograndis aqueous extracts applied to the seeds of U. decumbens and P. maximum. The 50% and 100% concentrations of leaf extract most strongly inhibited the germination, vigor and seedling growth of U. decumbens and P. maximum. The germination speed index and the root length were the characteristics that were most affected by the potentially allelopathic substances contained in the eucalyptus extracts at all concentrations.

Trinexapac-Ethyl and Sulfometuron-Methyl Selectivity to Young Eucalyptus Plants

Trinexapac-ethyl and sulfometuron-methyl are the most widely used ripeners in sugarcane. The application is performed by airborne spraying. Thus, if weather conditions are unfavorable, spray drift to neighboring areas may occur. The objective of this study was to assess the selectivity of the plant growth regulators trinexapac-ethyl and sulfometuron-methyl, used as sugarcane ripeners, to eucalyptus (Eucalyptus urograndis) young plants. The experiment was installed in an eucalyptus commercial yield area, in the municipality of Tambaú, state of São Paulo, Brazil, and arranged in a 2 x 8 factorial design in randomized blocks with four replications. The treatments studied were trinexapac-ethyl and sulfometuron-methyl, sprayed in eight doses, 0; 1.0; 2.5; 5.0; 10; 25; 50 and 100% of the dose used in sugarcane as ripeners (200 g ha-1 of trinexapac-ethyl and 15 g ha-1 of sulfometuron-methyl). Chemical ripeners were applied on eucalyptus plants with 48 cm in height on average; 10.1 branches; 4.5 mm of stem diameter and 44.3 cm of crown diameter, at 46 days after seeding. Trinexapac-ethyl was selective to eucalyptus and stimulated crown diameter growth. At higher doses, sulfometuron-methyl promoted severe noticeable injuries in eucalyptus plants, such as apical bud death. However, during the assessment period the plants recovered and the visual symptoms of phytotoxicity and growth alterations were not observed at 60 days after application. The plant growth regulators trinexapac-ethyl and sulfometuron-methyl were selective to eucalyptus young plants.

Extraction and Simultaneous Determination of Glyphosate, AMPA and Compounds of the Shikimic Acid Pathway in Plants

This study has aimed to develop a method for simultaneous extraction and determination by liquid chromatography and mass spectrometry (LC-MS/MS) of glyphosate, aminomethylphosphonic acid (AMPA), shikimic acid, quinic acid, phenylalanine, tyrosine and tryptophan. For the joint analysis of these compounds the best conditions of ionization in mass spectrometry and for chromatographic separation of the compounds were selected. Calibration curves and linearity ranges were also determined for each compound. Different extraction systems of the compounds were tested from plant tissues collected from sugarcane (Saccharum officinarum) and eucalyptus (Eucalyptus urophylla platiphylla) plants two days after the glyphosate application at the dose of 720 g a.e. ha-1. The plant material was dried in a forced air circulation drying oven and in a lyophilizer, and subsequently the extractions with acidified water (pH 2.5), acetonitrile-water (50:50) [v/v] and methanol-water (50:50) [v/v] were tested. To verify the recovery of the compounds in the plant matrix with acidified water as an extracting solution, the samples were fortified with a solution containing the mixture of the different analytical standards present so that this one presented the same levels of 50 and 100 μg L-1 of each compound. All experiments were conducted with three replicates. The analytical method developed was efficient for compounds quantifications. The extraction from the samples dried in an oven and using acidified water allowed better extraction levels for all compounds. The recovery levels of the compounds in the fortified samples with known amounts of each compound for both plants samples were rather satisfactory.

GLYPHOSATE DRIFT IN EUCALYPTUS PLANTS

ABSTRACTWith the present study we aim to assess the damage caused to Eucalyptus plants exposed to glyphosate drift in different canopy portions. The drift simulation was carried out through application of 1,080 g ha-1 of glyphosate in five canopy portions (0, 25, 50, 75 and 100% of the low branches), in four areas of cultivation. Areas I and II, plants with 0.91 and 2.98 m, and height of canopy drift exposition of 0.30 and 1.0 m, respectively. In areas III and IV both cultivations were 8.15 m high, varying the height of drift exposition between 2.0 and 2.5 m, respectively. At 30 and 480 days after application (DAA), the survival rate was assessed, and at 300 and 480 DAA diameter at breast height (DBH), height, volume and their respective increment were determined. The medium annual increment (MAI) was determined at 480 DAA. Area I, in which the plants were 0.91 m high, we observed that treatment with 100% of the low branches exposed to drift led to stand reduction of the plants around 18.75 and 38.19% at 30 and 480 DAA, respectively. Areas I and II showed reduction in plant growth in height and DBH, wood volume and MAI, to the extent that there was an increase in the portion of canopy exposed to glyphosate drift. However, in areas III and IV, in which 8.15 m height plants were found, no changes were verified for the evaluated characteristics, regardless of the portion of canopy exposed to glyphosate drift.