Selection of shrimp breeders free of white spot syndrome and infectious hypodermal and hematopoietic necrosis

The objective of this work was to select surviving breeders of Litopenaeus vannamei from white spot syndrome virus (WSSV) outbreak, adapted to local climatic conditions and negatively diagnosed for WSSV and infectious hypodermal and hematopoietic necrosis virus (IHHNV), and to evaluate if this strategy is a viable alternative for production in Santa Catarina, Brazil. A total of 800 males and 800 females were phenotypically selected in a farm pond. Nested-PCR analyses of 487 sexually mature females and 231 sexually mature males showed that 63% of the females and 55% of the males were infected with IHHNV. Animals free of IHHNV were tested for WSSV, and those considered double negative were used for breeding. The post-larvae produced were stocked in nine nursery tanks for analysis. From the 45 samples, with 50 post-larvae each, only two were positive for IHHNV and none for WSSV. Batches of larvae diagnosed free of virus by nested-PCR were sent to six farms. A comparative analysis was carried out in growth ponds, between local post-larvae and post-larvae from Northeast Brazil. Crabs (Chasmagnathus granulata), blue crabs (Callinectes sapidus), and sea hares (Aplysia brasiliana), which are possible vectors of these viruses, were also evaluated. The mean survival was 55% for local post-larvae against 23.4% for post-larvae from the Northeast. Sea hares showed prevalence of 50% and crabs of 67% of WSSV.

Conservação on farm da agrobiodiversidade de sítios familiares em Jequié, Bahia, Brasil

No Brasil, a Caatinga é área prioritária para conservação da biodiversidade, na qual muitas comunidades de agricultores mantêm práticas agrícolas fundamentais para a conservação on farm. O objetivo deste trabalho foi realizar um levantamento sobre o perfil socioeconômico e cultural de feirantes, identificando espécies e manejos em suas roças, que favoreçam a conservação on farm da diversidade de espécies cultivadas. Foi analisada a influência da área das roças sobre a riqueza e abundância de plantas. A pesquisa foi realizada por meio de visitas em feiras livres e unidades familiares de agricultores, localizadas no município de Jequié - BA, nordeste do Brasil. Foram citadas 28 espécies para fins alimentares, pertencentes a 17 famílias botânicas e totalizando 75 etnovariedades. A influência da área das roças sobre a riqueza teve baixa correlação (r = 0,29, p < 0,01), enquanto a abundância de plantas, forte correlação (r = 0,69, p < 0,01). A maioria dos agricultores tem pouca instrução, baixa renda e, nas roças estudadas, foi verificada a ocorrência de conservação on farm.

Lyme Disease: antibodies against Borrelia burgdorferi in farm workers in Argentina

Lyme Disease is a tick-borne (specially by Ixodes ticks) immune-mediated inflammatory disorder caused by a newly recognize spirochete, Borrelia burgdorferi. Indirect fluorescent antibody (IF) staining methods and enzyme-linked immunosorbent assay are frequently relied upon to confirm Lyme borreliosis infections. Although serologic testing for antibodies has limitations, it is still the only practical means of confirming B. burgdorferi infections. Because we have no previous report of Lyme disease in human inhabitants in Argentina, a study was designed as a seroepidemiologic investigation of the immune response to B. burgdorferi in farm workers of Argentina with arthritis symptoms. Three out of 28 sera were positive (#1,5 and 9). Serum # 1 was positive for Immunoglobulin G at dilution 1:320, serum # 5 and # 9 both to dilution 1:160; while for Immunoglobulin M all (#1, 5 and 9) were positive at low dilution (1:40) using IF. The results showed that antibodies against B. burgdorferi are present in an Argentinian population. Thus caution should be exercised in the clinical interpretation of arthritis until the presence of B. burgdorferi be confirmed by culture in specific media.

Vibrio vulnificus as a health hazard for shrimp consumers

Over the last 30 years, a number of Vibrio species found in the aquatic environment have been indicated as cause of disease in human beings. Vibrio vulnificus is an emergent pathogen, an invasive and lethal marine bacterium related to wound infection and held accountable for gastroenteritis and primary septicemia. It occurs quite frequently in marine organisms, mainly in mollusks. This study aimed at isolating and identifying strains of V. vulnificus based upon the analysis of twenty samples of seabob shrimp, Xiphopenaeus kroyeri (Heller), purchased at the Mucuripe fish market (Fortaleza, Brazil). TCBS agar was used to isolate suspect strains. Seven of twenty-nine strains isolated from six different samples were confirmed as such by means of biochemical evidence and thus submitted to biological assays to determine their virulence. The susceptibility of the V. vulnificus strains to a number of antibiotics was tested. None of the V. vulnificus strains showed signs of virulence during a 24-hour observation period, possibly due to the shedding of the capsules by the cells. As to the results of the antimicrobial susceptibility tests, the seven above-mentioned V. vulnificus strains were found to be sensitive to nitrofurantoin (NT), ciprofloxacin (CIP), gentamicin (GN) and chloramphenicol (CO) and resistant to clindamycin (CI), penicillin (PN) and ampicillin (AP).

Antibacterial activity of GUAVA, Psidium guajava Linnaeus, leaf extracts on diarrhea-causing enteric bacteria isolated from Seabob shrimp, Xiphopenaeus kroyeri (Heller)

Guava leaf tea of Psidium guajava Linnaeus is commonly used as a medicine against gastroenteritis and child diarrhea by those who cannot afford or do not have access to antibiotics. This study screened the antimicrobial effect of essential oils and methanol, hexane, ethyl acetate extracts from guava leaves. The extracts were tested against diarrhea-causing bacteria: Staphylococcus aureus, Salmonella spp. and Escherichia coli. Strains that were screened included isolates from seabob shrimp, Xiphopenaeus kroyeri (Heller) and laboratory-type strains. Of the bacteria tested, Staphylococcus aureus strains were most inhibited by the extracts. The methanol extract showed greatest bacterial inhibition. No statistically significant differences were observed between the tested extract concentrations and their effect. The essential oil extract showed inhibitory activity against S. aureus and Salmonella spp. The strains isolated from the shrimp showed some resistance to commercially available antibiotics. These data support the use of guava leaf-made medicines in diarrhea cases where access to commercial antibiotics is restricted. In conclusion, guava leaf extracts and essential oil are very active against S. aureus, thus making up important potential sources of new antimicrobial compounds.

High occurrence of giardiasis in children living on a 'landless farm workers' settlement in Araras, São Paulo, Brazil

Enteric parasitosis remains an important public health problem in many areas around the world including in Brazil, and it is frequently associated with poverty and lack of sanitation facilities. Research carried out over the course of a year revealed that 96.6% (28/29) of children randomly selected from a 'landless farm workers' settlement in Araras, São Paulo, aged 4 - 15 years, presented Giardia intestinalis cysts. After referral to the neighborhood Health Office, all the children received tinidazole, given as a single dose of 50 mg/kg and 12 months later, new fecal samples were collected and analyzed. Despite the low adherence to the study, a high percentage (64.3% - 9/14) of the children remained positive for the parasite. This study showed a high positivity of giardiasis in child residents of the settlement, even after treatment; adults were not sensitized to the study and did not collected and/or deliver children fecal samples. The precarious living conditions are consistent with a high susceptibility to parasitic diseases, suggesting that the treatment of the infected individuals without identifying and eradicating the means of contamination is simply a palliative measure.

Evaluation of larvicidal activity and brine shrimp toxicity of rhizome extracts of Zingiber zerumbet (L.) Smith

Introduction In this study, we used dichloromethane (DCM) and methanol (MeOH) extracts of the Zingiber zerumbet rhizome to evaluate brine shrimp lethality and larvicidal activity on Aedes aegypti and Anopheles nuneztovari mosquitoes. Methods Bioassays were performed by exposing third-instar larvae of each mosquito species to the DCM or MeOH extracts. Results Probit analysis with DCM and MeOH extracts demonstrated efficient larvicidal activity against A. aegypti and A. nuneztovari larvae. Conclusions The DCM and MeOH extracts showed higher activity against A. nuneztovari larvae than against A. aegypti larvae, suggesting that the extracts have species-specific activity.

Screening of plants found in Amazonas State for lethality towards brine shrimp

226 methanol and water extracts representing 74 mainly native plant species found in Amazonas State, Brazil, were tested at a standard concentration of 500 μg/mL for lethality towards larvae of the brine shrimp species Artemia franciscana. Several cytotoxic plant species were identified in this work: Aspidosperma marcgravianum, A. nitidum, Croton cajucara, Citrus limetta, Geissospermum argenteum, Minquartia guianensis, Piper aduncum, P. amapense, P. capitarianum, P. tuberculatum and Protium aracouchini. The results were analyzed within the context of the available traditional knowledge and uses for these plants.

Spatio-temporal variation of the pink shrimp Farfantepenaeus paulensis (Crustacea, Decapoda, Penaeidae) associated to the seasonal overture of the sandbar in a subtropical lagoon

On the southern Brazilian coast, the penaeid pink shrimp Farfantepenaeus paulensis (Pérez-Farfante, 1967) is intensively captured by both artisanal and industrial fisheries. In the Ibiraquera Lagoon, artisanal fishing of juvenile stocks has declined sharply over the last 15 years. The traditional management methods used by fishermen are no longer sustainable due to conflicts with the demands of tourism and weak environmental and public agencies. The dynamics of the timing of the artificial opening and natural reformation of a sandbar across the mouth of the lagoon are regarded as a central socio-ecological problem. We evaluated the abundance and biomass variation of F. paulensis throughout the year and along a spatial gradient measured from the sandbar into the lagoon. We also explored the influence of opening the sandbar on the shrimp population. Samples were collected monthly from February 2004 to February 2005, using a 27 mm mesh cast net, in four different areas, with three sites being sampled per area. Small numbers of large shrimps were captured in the upper area, while greater biomass and abundance of small shrimps were observed near the sandbar, suggesting the role of the inner area as a refuge for growing. The highest records for biomass and abundance were recorded during warm months, regardless if the lagoon was open or closed by the sandbar. The Ibiraquera Lagoon could be more productive for fishermen if the sandbar opening was coincident to the period of natural post-larvae influx. Furthermore, coastal stocks could be improved by opening the sandbar again during summer in conjunction with fishing quotas.

Population dynamics of the pea crab Austinixa aidae (Brachyura, Pinnotheridae): a symbiotic of the ghost shrimp Callichirus major (Thalassinidea, Callianassidae) from the southwestern Atlantic

The Pinnotheridae family is one of the most diverse and complex groups of brachyuran crabs, many of them symbionts of a wide variety of invertebrates. The present study describes the population dynamics of the pea crab Austinixa aidae (Righi, 1967), a symbiont associated with the burrows of the ghost shrimp Callichirus major (Say, 1818). Individuals (n = 588) were collected bimonthly from May, 2005 to September, 2006 along a sandy beach in the southwestern Atlantic, state of São Paulo, Brazil. Our data indicated that the population demography of A. aidae was characterized by a bimodal size-frequency distribution (between 2.0 and 4.0 mm and between 8.0 and 9.0 mm CW) that remained similar throughout the study period. Sex ratio does not differ significantly from 1:1 (p > 0.05), which confirms the pattern observed in other symbiontic pinnotherids. Density values (1.72 ± 1.34 ind. • ap.-1) are in agreement with those found for other species of the genus. The mean symbiosis incidence (75.6%) was one of the highest among species of the Pinnotheridae family, but it was the lowest among the three studied species of the genus. Recruitment pattern was annual, beginning in May and peaking in July, in both years, after the peak of ovigerous females in the population (from March to May). Our findings describe ecological and biological aspects of A. aidae similar to those of other species of this genus, even from different geographic localities.

Taxonomic revision of doubtful Brazilian freshwater shrimp species of genus Macrobrachium (Decapoda, Palaemonidae)

The freshwater prawns of the genus Macrobrachium Spence Bate, 1868 are widely distributed in rivers of tropical and subtropical regions and represent an interesting group with controversial taxonomy. The morphological characters traditionally used to separate species have shown a high intraspecific variation. Doubts about the status of M. birai Lobão, Melo & Fernandes, 1986, M. holthuisi Genofre & Lobão, 1978 and M. petronioi Melo, Lobão & Fernandes, 1986 have been arisen due to the high resemblance of the former two species with M. olfersi (Wiegmann, 1836), and the latter one with M. potiuna (Müller, 1880). Therefore, we performed a detailed morphological analysis of these species, including new characters not usually used in the species recognition. The present results here with molecular data lead us to conclude that M. birai and M. holthuisi are junior synonyms of M. olfersi, and M. petronioi is a junior synonym of M. potiuna. Considering these synonymies, 17 valid species are now reported for the Brazilian territory.

Relative growth and sexual maturity of the freshwater shrimp Palaemon pandaliformis (Crustacea, Palaemonidae) in northeastern of Brazil (Canavieiras, Bahia)

This study comprises the description of relative growth and sexual maturity of a population of Palaemon pandaliformis Stimpson, 1871 in Salsa River (Northeastern Brazil). Samples were collected monthly between September 2009 and August 2010. Females were larger, heavier, and showed a greater allometric coefficient (b) than male specimens. Only carapace length vs. pleura length in females presented a significant difference in the relative growth pattern, indicating a puberty moult. This relationship is strictly correlated to reproduction and its success rate in female shrimps. Estimated carapace length in 50% of mature females (CL50) was 4.53 mm. It was not possible to compare obtained CL50 results due to a lack of studies on this species. Comparison was based on the size of the smallest captured ovigerous female (3.81 CL mm), which is within the scope of recorded size for estuaries located in higher latitudes. This study reveals the lack of research on this genre in freshwater environments on a national and global scale.

Produção de inoculante micorrízico on farm utilizando resíduos da indústria florestal

A produção de inoculante à base de fungos micorrízicos arbusculares (FMAs) utilizando o método on farm é uma alternativa para estimular o uso de inoculante microbiano no sistema de produção vegetal e reduzir os custos associados com a compra desse produto. O objetivo deste estudo foi avaliar o potencial de resíduos do setor florestal, a casca de Pinus (CP) e o lodo de celulose (LC), como componentes do substrato para a produção de inoculante micorrízico on farm . Plantas de sorgo pré-colonizadas com os FMAs, Claroideoglomus etunicatum RJN101A e Dentiscutata heterogama PNB102A, foram estabelecidas em casa de vegetação por três meses em substrato formado por areia:argila expandida:solo (2:2:1). Após, essas foram transplantadas para sacos plásticos de 20 L contendo substrato formado por CP ou LC, misturados com casca de arroz carbonizada + solo de barranco (1:1:1). O experimento seguiu um fatorial 2 × 2, sendo dois isolados fúngicos e dois resíduos, com cinco repetições, em delineamento inteiramente casualizado. As plantas cresceram por três meses sob condições ambientais e, após esse período, o substrato foi analisado quanto ao número de esporos de FMAs, à colonização micorrízica da planta hospedeira e ao potencial de inóculo pelo método do NMP (número mais provável). O substrato foi dividido em três camadas (superior, mediana e inferior) e apenas o número de esporos foi avaliado individualmente para cada camada. O número de esporos de ambos FMAs não foi influenciado pelo tipo resíduo, mas diminuiu da parte superior para a inferior dentro de cada unidade experimental. D. heterogama tendeu a produzir maior número de esporos do que C. etunicatum . A porcentagem de colonização micorrízica do sorgo pelos FMAs foi significativamente maior no resíduo LC do que CP. O número de propágulos infectivos de FMAs tendeu também a ser maior em LC (22 a 28 propágulos cm-3 substrato) do que em CP (1,6 a 6,5 propágulos cm-3 substrato). O resíduo LC tem potencial para ser utilizado como componente do substrato em sistemas de produção de inoculante micorrízico on farm.

Yield and performance of sugarcane in on-farm interface with rubber in Brazil

Agroforestry systems are indicated as an alternative for sugarcane (Saccharum officinarum) cultivation in Piracicaba, SP, Brazil, however there are not many field experiments on plant performance under these conditions in the world. The objective of this work was to assess crop yield and partitioning in a sugarcane-rubber (Hevea brasiliensis) interface in on-farm conditions. The availability of irradiance for the crop along the interface was simulated and its effe ct over sugarcane dry matter production was tested. Crop yield was negatively affected by distance of the trees, but development and sucrose were not affected. Above ground dry matter increased from 16.6 to 51.5 t ha-1 from trees. Partitioning did not have a defined standard, as harvest index increased from 0.85 to 0.93, but specific leaf area was not significant along the transect, ranging from 13.48 to 15.73 m² kg-1. Light is the main factor of competition between the trees and the crop, but the relative importance of below ground interactions increases closer to the trees. Feasibility of the system depends on maturity of the trees and management strategies.

Time-related action of Lactobacillus plantarum in the bacterial microbiota of shrimp digestive tract and its action as immunostimulant

The objective of this work was to assess the time-related action of probiotic Lactobacillus plantarum in the bacterial microbiota of the digestive tract of Litopenaeus vannamei, and the relation of total haemocyte count and serum phenol oxidase activity of shrimp challenged with Vibrio harveyi. Shrimps were fed with a probiotic-supplemented diet, for eight days, then shifted to a commercial diet. Shrimps fed only with the commercial diet served as control. Evaluations were made on the 8th day of experiment and repeated two, four, six and eight days later. Total lactic bacteria in the digestive tract was higher until the 4th day of evaluation in the probiotic-supplemented group. Vibrio spp. counts were higher in the control at days zero and two. Until the 4th day of evaluation, the total haemocyte counts in shrimps after challenge with V. harveyi were higher in probiotic-supplemented group than in control group. Significant difference was not observed in phenol oxidase activity. On the 6th day after shifting from supplemented to control diet, all parameters were equal in both groups, suggesting that the time-related action of L. plantarum in shrimp is short.