In vitro ANTIGIARDIAL ACTIVITY OF THE CYSTEINE PROTEASE INHIBITOR E-64

The quest for new antiparasitic alternatives has led researchers to base their studies on insights into biology, host-parasite interactions and pathogenesis. In this context, proteases and their inhibitors are focused, respectively, as druggable targets and new therapy alternatives. Herein, we proposed to evaluate the in vitro effect of the cysteine protease inhibitor E-64 on Giardia trophozoites growth, adherence and viability. Trophozoites (105) were exposed to E-64 at different final concentrations, for 24, 48 and 72 h at 37 °C. In the growth and adherence assays, the number of trophozoites was estimated microscopically in a haemocytometer, whereas cell viability was evaluated by a dye-reduction assay using MTT. The E-64 inhibitor showed effect on growth, adherence and viability of trophozoites, however, its better performance was detected in the 100 µM-treated cultures. Although metronidazole was more effective, the E-64 was shown to be able to inhibit growth, adherence and viability rates by ≥ 50%. These results reveal that E-64 can interfere in some crucial processes to the parasite survival and they open perspectives for future investigations in order to confirm the real antigiardial potential of the protease inhibitors.

CARACTERÍSTICAS DA GERMINAÇÃO DE SEMENTES DE 64 ESPÉCIES DE LEGUMINOSAS FLORESTAIS NATIVAS DA AMAZÔNIA, EM CONDIÇÕES DE VIVEIRO

Foram estudados aspectos da germinação de sementes de 64 espécies de leguminosas, nativas da Amazônia, sendo 52 arbóreas, 10 cipós, 1 arbusto e 1 herbácea, oriundas de áreas de terra firme ou periodicamente inundadas. Cinquenta e oito por cento das espécies apresentaram, sem tratamento pré-germinativo, percentagens de germinação maiores que 70% num período menor que 91 dias. Em 8 espécies de tegumento duro as percentagens de germinação, sem pré-tratamento, encontradas foram menores que 40%. Cinquenta e nove por cento das espécies de áreas inundadas apresentaram uma germinação tipo hipógea enquanto, cinquenta e quatro por cento das espécies de terra firme apresentaram uma germinação epigea. Poliembrionia ocorreu em 22% das espécies. Sementes com comprimento maior que 3,1 cm só apresentaram germinação hipógea enquanto as sementes com comprimento menor ou igual a 1 cm só apresentaram germinação epigea.

Origem Anômala da Coronária (ALCAPA) em tomógrafo de 64 canais

A tomografia computadorizada multidetector (TCMD) com 64 canais disponibiliza para a prática clínica um excelente método para detecção de anomalias das artérias coronárias. O diagnóstico da anomalia coronariana consistindo da origem da artéria coronária esquerda no tronco pulmonar em adulto sem história prévia de doença congênita apresenta escassa casuística na literatura. Realizamos um relato de caso em uma paciente feminina de 30 anos de idade, com queixas de cansaço aos grandes esforços e cintilografia positiva para isquemia. O diagnóstico foi realizado pela TCMD de 64 canais e com isso verifica-se que o método pode ser utilizado como de primeira linha.

The retained capacity of Lutzomya longipalpis (Lutz & Neiva) to transmit Leishmania chagasi (Cunha & Chagas) after eight years (64 generations in a closed laboratory colony

A closed Lutzomyia longipalpis colony, from Ceará has been used to transmit Leishmania chagasi isolated from a fox in Pará state. The last time this colony was successfully used in similar transmission experiments was eight years (64 generations) ago indicating that this colony of Lu. longipalpis has fully maintained its vectorial capacity in spite of such a long period of maintainance in the laboratory.

CL 64, 855, a potent do anti-Trypanosoma cruzi drug, is also mutagenic in the salmonella/microsome assay

The nitroimidazole-tiadiazole derivative CL 64,855 (2-amino-5-(1-methyl-5-nitro-2-imidazolyl)-1,3,4-thiadiazole, a potent anti-trypanosomal drug, was assayed in a short-term bacterial mutagenicity test with Salmonella typhimurium strains TA 98, TA 100 and TA 102. Results indicate that CL 64,855 is a potent frameshift mutagen detected by strains TA 98 and TA 102. CL 64,855 was able to revert the indicators strains at concentrations as low as 0.1 µg/plate. Metabolic activation experiments with rat liver microsomal fractions did not increase the mutagenic action of Cl 64,855.

Soil Compaction Curve of an Oxisol Under Sugarcane Planted After In-row Deep Tillage

ABSTRACT Soil tillage that maintains the productivity of sugarcane plantations, providing an area for the root development and without traffic on crop rows, has given rise to new technologies in rural areas. The purpose of this study was to evaluate the soil physical properties in two sugarcane plantations, one of which was prepared with deep tilling and the other with conventional tillage. The experiment was conducted in Lençóis Paulista, São Paulo State. Soil penetration resistance and relative density were analyzed. The cone index was lower in deep-tilled soil without traffic in all layers, than in deep-tilled soil with traffic and in conventional tillage. In both tillage treatments, the relative density values were acceptable in the 0.00-0.15 m soil layer, but considered detrimental for sugarcane development in the 0.15-0.30 and 0.30-0.45 m layers.

Nitrogen and Potassium in Narrow-Row Cotton

ABSTRACT Information on fertilizer management for cotton in narrow-row cropping system is scarce; therefore, studies are needed to improve nutrient stewardship for such systems. The aim of this study was to evaluate the effects of nitrogen and potassium application on yield and fiber quality of cotton under a narrow-row system. A field trial was carried out for three years, where the treatments were set up in an incomplete factorial arrangement [(4 × 4) + 1] under a randomized block design, with four N rates (20, 40, 60, and 80 kg ha-1), four K2O rates (0, 40, 80, and 120 kg ha-1), and one control (no N or K2O), for a total of 17 treatments, with four replicates. Urea and potassium chloride were applied on the soil surface 20 days after crop emergence. Varieties used were FMT 701 (2009/2010 and 2010/2011) and FMT 709 (2011/2012). Cotton yield and fiber quality parameters were measured. In the narrow-row cropping system, cotton lint yield was positively affected by N and K application. Cotton yield in relation to K applications was not dependent on N rates. Potassium application increased the micronaire index and fiber resistance, whereas high N rates reduced fiber resistance.

Influence of row spacing reduction on maize grain yield in regions with a short summer

The interest in reducing maize row spacing in the short growing season regions of Brazil is increasing due to potential advantages such as higher radiation use efficiency. This experiment was conducted to evaluate the effect of row spacing reduction on grain yield of different maize cultivars planted at different dates. The trial was conducted in Lages, in the State of Santa Catarina, Brazil, during 1996/97 and 1997/98 growing seasons, in a split-split plot design. Early (October 1st) and normal (November 15) planting dates were tested in the main plot; two morphologically contrasting cultivars (an early single-cross and a late double-cross hybrids) were evaluated in the split plots and three row widths (100, 75 and 50 cm) were studied in the split-split plots. The reduction of row spacing from 100 to 50 cm increased linearly maize grain yield. The yield edge provided by narrow rows was higher when maize was sown earlier in the season. Differences in hybrid cycle and plant architecture did not alter maize response to the reduction of row spacing.

Popping expansion and yield responses of popcorn cultivars under different row spacings and plant populations

The objective of this work was to evaluate the agronomic traits and the popping expansion index of three Brazilian popcorn cultivars under different row spacings and plant populations. The trials were performed during two crop seasons, under field conditions. The experimental design used was a randomized complete block, in a split-split plot, with 27 treatments and four replicates. Treatments were represented in a triple factorial arrangement: three row spacings (0.40, 0.60, and 0.80 m), three plant populations (40,000, 60,000, and 80,000 plants per hectare), and three popcorn cultivars (IAC-TC 01, IAC 12, and Zelia). The increase in plant population causes a reduction in the number of grains per ear, lower prolificacy, and grain weight loss. Cultivar grain yield is affected by row spacing and popcorn plant population. Cultivar IAC 12 shows highest grain yield under row spacings of 0.40 and 0.60 m and plant population between 60,000 and 80,000 plants per hectare. The popping expansion index is not affected by row spacing or plant population.

Multidetector-row computed tomography in the preoperative diagnosis of intestinal complications caused by clinically unsuspected ingested dietary foreign bodies: a case series emphasizing the use of volume rendering techniques

Objective The present study was aimed at describing a case series where a preoperative diagnosis of intestinal complications secondary to accidentally ingested dietary foreign bodies was made by multidetector-row computed tomography (MDCT), with emphasis on complementary findings yielded by volume rendering techniques (VRT) and curved multiplanar reconstructions (MPR). Materials and Methods The authors retrospectively assessed five patients with surgically confirmed intestinal complications (perforation and /or obstruction) secondary to unsuspected ingested dietary foreign bodies, consecutively assisted in their institution between 2010 and 2012. Demographic, clinical, laboratory and radiological data were analyzed. VRT and curved MPR were subsequently performed. Results Preoperative diagnosis of intestinal complications was originally performed in all cases. In one case the presence of a foreign body was not initially identified as the causal factor, and the use of complementary techniques facilitated its retrospective identification. In all cases these tools allowed a better depiction of the entire foreign bodies on a single image section, contributing to the assessment of their morphology. Conclusion Although the use of complementary techniques has not had a direct impact on diagnostic performance in most cases of this series, they may provide a better depiction of foreign bodies' morphology on a single image section.