POTENCIAL DA ASSOCIAÇÃO Pisolithus microcarpus COM MUDAS DE Corymbia citriodora EM SOLO CONTAMINADO COM ZINCO

O uso de plantas com potencial de associação com microrganismos é uma prática frequente em solos contaminados por metais pesados, considerada de baixo custo e ambientalmente correta. O trabalho objetivou avaliar o crescimento do Corymbia citriodora (Hook.) K.D. Hill & L.A.S. Johnson e o efeito da inoculação com Pisolithus microcarpus UFSC-Pt116 em solo contaminado com Zn. O delineamento experimental foi inteiramente casualizado em arranjo fatorial (2 x 6), sendo com e sem inóculo e seis doses de Zn (0, 300, 600, 900, 1200 e 1500 mg kg-1 de solo), com seis repetições. As mudas foram inoculadas e cultivadas durante 90 dias em viveiro. Após 67 dias do transplante definitivo foi avaliado o percentual de colonização ectomicorrízica, a altura de planta, diâmetro do colo, número de folhas, índice de clorofila total, volume radicular, massa seca das folhas, da haste caulinar, radicular e total, relação massa seca aérea/massa seca radicular e a relação altura/diâmetro do colo. O percentual de colonização ectomicorrízica em Corymbia citriodora é estimulado pelo acréscimo de até 1412,21 mg kg-1 de Zn no solo. O Corymbia citriodora é tolerante a adição de até 1500 mg kg-1 de zinco em solo com 81% de argila, mesmo sem a inoculação com Pisolithus microcarpus. A análise de correlação canônica evidencia que a inoculação com P. microcarpus favorece a massa seca total, radicular e da parte aérea de Corymbia citriodora cultivado em solo com 81% de argila contaminada com 600 mg kg-1 de Zn.

Improved production of chlorogenic acid from cell suspension cultures of Lonicera macranthoids

Purpose: To evaluate the potential of Lonicera macranthoids Hand. -Mazz. Yulei1 suspension culture system for enhanced production of the main secondary metabolite, chlorogenic acid. Methods: The callus of L. macranthoides Hand.-Mazz. “Yulei1” was suspension cultured in B5 liquid medium supplemented with different plant growth regulators. Biomass accumulation was calculated by weight method and chlorogenic acid production was measured using high performance liquid chromatography (HPLC). HPLC was carried out on C18 analytical column at 35 °C and the detection wavelength was set at 324 nm. Results: The results showed that maximum accumulation of biomass and chlorogenic acid were achieved 15 days after culture growth. The optimized conditions for biomass accumulation and chlorogenic acid production were 50 g/L of inoculum on fresh weight basis, B5 medium supplemented with plant growth regulators, 30 - 40 g/L sucrose and initial medium pH of 5.5. Maximum accumulation of chlorogenic acid and biomass were observed when the culture medium was supplemented with 2.0 mg/L6-BA. Optimal accumulation of chlorogenic acid was observed using combination of hormones 2.0 mg/L 6-Benzyladenine (BA) + 0.5 mg/L2, 4-Dichlorophenoxyacetic acid (2,4-D), while optimal accumulation of biomass was observed with 2.0 mg/L 6-BA + 2.0 mg/L2, 4-D. In addition, phenylalanine also contributed to the synthesis of chlorogenic acid at a concentration > 50 mg/L. Conclusion: Cell suspension cultures of L. macranthoides Hand.-Mazz. “Yulei1” have successfully been established. The findings provide a potential basis for large scale production of chlorogenic acid using cell suspension cultures of L. macranthoides.