Removable orthodontic appliances: frequency and cleaning agents used by students and recommended by dentists

Aim: To evaluate the prevalence and hygiene habits of 13-19 years-old adolescent users of removable orthodontic appliances (ROA) and to determine hygiene methods for the appliances prescribed by dentists, in the city of Pelotas. Methods: The study had two stages. The first stage was a telephone interview with dentists. Dentists were interview by telephone calls in order to obtain information regarding the hygiene methods for cleaning acrylic appliances. Second stage was a cross-sectional study performed with schoolchildren. Children from public and private schools with secondary level were included in the sample. A questionnaire was applied to the students using any type of ROA. Questionnaires included demographic information and behavioral characteristics. Data collected were subjected to Chi-square test and logistic regression. Results: The prevalence of children using ROA was 5.4%. Students (89.7%) and dentists (47.2%) reported to prefer mechanical methods to clean their ROA. Cleaning with soup, hydrogen peroxide or effervescent tabs were less used. High frequency of use was associated with higher frequency of hygiene on the ROA. Conclusions: The prevalence of schoolchildren using removable appliances was low. The common cleaning method used by children and prescribed by dentists was mechanical. Hygiene frequency was significantly associated with the routine of use of the appliance and with the type of hygiene method.

Affinity purification of recombinant human plasminogen activator from transgenic rabbit milk using a novel polyolresponsive monoclonal antibody

Purpose: To develop processes for effective isolation and purification of recombinant human plasminogen activator (rhPA) from transgenic rabbit milk. Methods: Immunoaffinity chromatography was selected and improved by a special polyol-responsive monoclonal antibody (PR-mAb). Alteplase was used as immunogen because of its similarity to rhPA in terms of structure. The PR-mAb was prepared by hybridoma technology and screened by ELISA-elution assay. Screening antibody was performed using rhPA milk in an ELISA-elution assay. The antibody clone C4-PR-mAb was selected for immunoaffinity chromatography. The rhPA was effectively bound to immobilized C4-PR-mAb on the column and was eluted with Tris buffer comprising 0.75 mol/L ammonium sulfate and 40n% propanediol (pH7.9). The rhPA was further purified by passing through Chromdex75 gel filtration column. Results: There were 12 hybridoma strains selected into the polyol-responsive mAbs screen step and three hybridoma strains were superior for producing PR-mAbs (C1, C4, C8). The rhPA can be purified from transgenic rabbit milk and maintained a higher thrombolytic activity in vitro by FAPA. Conclusion: The results demonstrate the suitability of the alternative approach used in this study. Using immunoaffinity chromatography and gel filtration column is feasible and convenient for extracting rhPA from milk, and should be useful for purifying other tPA mutants or other novel recombinant milkderived proteins.