Essential – Mineral Balance on Growing Male Cattle

An experiment was designed to study the effect of unconventional protein source on the essential minerals balances of growing cattle.  The experiment had been conducted by experimental method with Completely Randomized Block Design, on twelve growing male cattle of Ongole Grade.  Based on the body weight, the animal trial was separated to three blocks, as the replication.  The tested treatment was four kind of protein sources in the ration (R):  R1 = kapok – seeds cakes; R2 = lamtoro – leave meals; R3 = cacao – pod meals and R4 = goat’s faeces.  The compose of ration was 50 percent of Elephant grass and 50 percent of concentrate (DM basis).  The measured variables were: the essential mineral balances i.e. Calcium, Phosphorus, Sodium, Potasium, Chlorine, Magnesium, Sulphur and Nitrogen.  The balance – trial was conducted by the total collection method.  The data was analyzed by the analysis of variance, and that of the interaction (Synergistic and antagonistic) by the Regression Analysis.  The result indicated that the total of essential mineral balance of each protein source or each ration on growing cattle of Ongole Grade is:  Cacao – pod meals = 116.09 g/day; Goat’s faeces = 111.89 g/day; lamtoro – leave meal = 84.64 g/day and Kapok – seed cake = 78.55 g/day.  These phenomena shown that all of the animal trial was in normal growth.  The strong synergistic interaction was reached by Ca « P (P <0.01; R2 = 0.92 – 0.98) while the weak interaction by Na « Cl (P > 0.05; R2 = 0.03 – 0.05).  Among the five couples of mineral elements of the antagonistic interaction the strong interaction was N ® S (P < 0.01; R2 = 0.70 – 0.75), while the weak interaction was Na ® P (P > 0.05; R2 = 0.08).  Based on all the variables measured, the experiment is concluded that: (1) all of the unconventional protein sources are able to improve the quality of the ration, indicated by the positive essential mineral balance on growing cattle of Ongole grade; (2) based on the total essential mineral balance, the most suitable protein source for growing cattle is cacao – pod meals; (3) the strong synergistic interaction was reached by Ca <----> P, while the antagonistic interaction was N ----> S.  (Animal Production 6(2): 101-109 (2004) Key Words: mineral, protein source, Ongole, cattle

Quality of Chicken Feather Processed in Different Conditions

Abstract. The objective of this research was to evaluate the hydrolyzed chicken feather based on pepsin digestibility and nutrient content, after physico-chemical and biological process. It was carried out by experimental methods at feed and nutrition laboratory. The treatments were hydrolyzed feather meals immersed in 0.5% NaOH and Na2S solution for 0, 2, 4, 6 and 8 hours, each treatment was repeated three times. The results showed that chemical treatment (NaOH-Na2S) in various time of incubation at 60oC followed by fermentation using Bacillus sp. MTS at 37oC for four days decreased the protein of hydrolyzed feather (78.88 to 73.06%), but increased the keratin fiber (1.9 to 3.26%). Pepsin digestibility informed that the increasing incubation time from 0, 2, 4, 6 to 8 hours resulted in higher solubility than that of control (30.2% at 8 hours vs 15.4% at 0 hours). Processing chicken feather  by  0.5% NaOH and Na2S solution at 60oC for 6 hours followed by fermentation increased the value of pepsin digestibility.  Key words: hydrolyzed, Bacillus sp. MTS, feather, solubility Abstrak. Penelitian ini bertujuan mengevaluasi kualitas nutrien tepung bulu ayam hasil proses hidrolisis secara fisiko-kimia dan biologis menggunakan Bacillus sp. MTS. Metode eksperimental digunakan dalam penelitian yang menggunakan dua tahap proses hidrolisis yaitu tahap 1: setelah perebusan bulu dalam larutan NaOH maka bulu direndam dalam larutan  0.5% NaOH dan Na2S pada 600C dan tahap 2: fermentasi bulu selama empat hari pada suhu 370C. Perlakuan berupa waktu inkubasi yaitu 0, 2, 4, 6 dan 8 jam diterapkan pada tahap kedua dengan ulangan sebanyak tiga kali. Perlakuan fisiko-kimia yang dilanjutkan fermentasi menggunakan bakteri spesifik penghasil enzim-enzim pendegradasi keratin bulu menurunkan kadar  protein tepung bulu  (78,88% menjadi 73,06%) dan meningkatkan kadar serat tepung bulu (1,9 menjadi 3,26%). Uji kelarutan protein tepung bulu dalam pepsin menginfromasikan bahwa proses tahap 1 menghasilkan nilai kelarutan protein tepung bulu yang meningkat dua kali dibanding kontrol (30,2% pada 8 jam vs 15,4% pada 0 jam inkubasi) atau enam kali dibanding tepung bulu tanpa hidrolisis (5%). Pengolahan bulu ayam menggunakan cara pemanasan, perendaman dalam larutan NaOH dan Na2S selama 6 jam pada 600C serta fermentasi menghasilkan tepung bulu dengan daya larut dalam pepsin  lebih baik dibanding tanpa pengolahan.  Kata kunci: hidrolisis, tepung-bulu, Bacillus sp. MTS, kelarutan