The Effects of Branched Chain Volatile Fatty Acids on Reduced Sugar and Branched Chain Amino Acid Concentration of Substrates that Fermented by Aspergillus oryzae

A study was conducted to know the reduced sugar and branched chain amino acids concentration in substrate that fermented by Aspergillus oryzae. Branched chain amino acids represent amino acids that are very important for microorganism development, including yeast and ruminal microorganism as well as for the growth of the ruminant animal. The study was conducted using Completely Randomized Design (CRD). There were five kinds of supplements that were added into the media. So, that this experiment were A: control, B: A + 0.5% urea, C: B + 1% extract of cassava leaves, D: C + 1% isobutyrate, and E: D + 1.3% 2-methilbutyrate. There were five replicates in each treatment. The measured variables in these study were, colonies cell biomass of A. oryzae, reduced sugar, Crude Protein, and branched chain amino acid concentration. The results showed that the highest number of colonies, concentration of reduce sugar, and concentration of branched chain amino acids was obtain from the substrate of treatments D. (Animal Production 4(2): 83-88 (2002) Key words : Branched Chain Amino Acids, Branched Chain Volatile Fatty Acids, Aspergillus oryzae

Increasing of Energy Quality of Waste Product with Trichoderma and Aspergillus

Biomasa of agricultural residues are potensial as ruminant feeds. However due it is low palatability, digestibility and nutritive value limited their use. In order to improve their use, treatment need to be applied. Biological treatment by using microba seems to be an alternative because of their capability with no pollution problems. The first experiment aims to select the microorganism which have a potensial to degrade the crude fiber, based the production of reduction sugar. The second experiment aims to improve the protein and amino acid on rice straw, cassava, waste, and rice husk, by inoculated the starter of Candida utilis and or Sacharomyces cerevise. The second experiment has been conducted on Animal Nutrition and Feed Laboratory, Faculty of Animal Husbandry UNSOED for eight month Fermentation trial has been done in semi solid media, by the method of Kjic (1964), in Batch System, Variables measure were: (1) reduction sugar, (2) cellulose, (3) protein, (4) amino acids, (5) cellulase activity, (6) essensial mineral and (7) energy. Based on the all variables measured that were conclused that the quality of  rice straw can be improved by mixed culture of  T, viride – S. cerevise, the rice husk by A. niger – C. utilis, T. viride – C. utilis and A. niger – S cerevise while for cassava waste by  A. niger – S. cerevise and A. niger – C. utilis  (Animal Production 1(1) : 10-16 (1999). Key Words: Waste Product, Energy, Microorganism