Establishment of a PCR and restriction enzyme assay for the detection of the hemochromatosis gene in normal subjects and diabetic patients

The purpose of this study was to establish a polymerase chain reaction (PCR)-restriction enzyme assay for detecting the hereditary hemochromatosis (HHC) mutation, C282Y, in gestational and gestational diabetic subjects in South Florida. DNA samples from 43 gestational subjects were amplified by PCR, digested with RsaI, and analyzed by electrophoresis. An allelic frequency of 2.33%, or 4.65% heterozygosity, was observed. The assay is successful and applicable to future studies on HHC and gestational diabetes. ^

Smallpox vaccination, the establishment of vaccination boards, and state formation in Venezuela and Cuba in the nineteenth century

The Balmis expedition, sent to America by the Spanish monarch Charles IV in 1803, was a watershed in the history of Medicine as it made smallpox vaccination available for the first time, effectively prevented the disease from spreading, and saved thousands of lives. Immunization required complex administrative measures and political decisions including the creation of Vaccination Boards, all of which involved different sectors of Spanish American society. This dissertation argues that at the beginning of the nineteenth century the Spanish American colonial state had reached some level of maturity and cohesion that made it capable of executing this complex project in public health. The significance of this mobilization and the every-day experience in implementing this new public health measure is the center of this work. It is situated geographically in Venezuela and Cuba, entities which took different evolutionary paths in the nineteenth century. The organization and functioning of Vaccination Boards in these two areas are used to illustrate the state formation process, and sharp political differences in this critical period.

Hydrologic processes on tree islands in the Everglades (Florida, USA): tracking the effects of tree establishment and growth

The hydrodynamics of tree islands during the growth of newly planted trees has been found to be influenced by both vegetation biomass and geologic conditions. From July 2007 through June 2009, groundwater and surface-water levels were monitored on eight recently planted tree islands at the Loxahatchee Impoundment Landscape Assessment (LILA) facility in Boynton Beach, Florida, USA. Over the 2-year study, stand development coincided with the development of a water-table depression in the center of each of the islands that was bounded by a hydraulic divide along the edges. The water-table depression was greater in islands composed of limestone as compared to those composed of peat. The findings of this study suggest that groundwater evapotranspiration by trees on tree islands creates complex hydrologic interactions between the shallow groundwater in tree islands and the surrounding surface water and groundwater bodies.

Perceptions of the Beach Users: A Case Study of the Coastal Areas of North Cyprus Towards Establishment of a "Carrying Capacity"

Within the main elements of economic sustainability, socio-cultural sustainability, and environmental sustainability, the criteria of 'carrying capacity’ have ben emphasized through residents’ perception analysis to explore practical methods towards the application and implementation of such criteria. As data analysis revealed, the main tourist resources in the case of North Cyprus –the coast and the beach- have a certain capacity to sustain the impact and pressure of tourism. Despite the significance of the indigenous environment and with respect to the residents’ perception of optimum carrying capacity levels, this issue has not been given a due consideration. This has resulted in a process of coastal development which bypasses any measure ore application of a standard to harmonize the degree of physical development and the capacity of the beach. The main objective of this paper is to establish the concept of ‘carrying capacity’ as the means to achieve the reconciliation of environmental impacts with tourism development. The study concludes that, if carrying capacity measurement and its implementation are not incorporated into the planning decision as a clear policy, there will be grave negative consequences for those resources attracting visitors.

A study for the establishment of higher foodservice standards in health-care facilities

1. Area of Investigation The relative need of higher standards in healthcare foodservice facilities. Individual opinions of foodservice directors. 2. Materials and Methods Used Existing text, trade publications, interviews with industry leaders, conferences with estemmed professors and the author's own experience were used for research and evaluation. An important method used to evaluate the need for higher standards was a descriptive survey and the analysis thereof. 3. Major Findings A. The survey showed, with a 42 percent response, that the overwhelming majority of the respondents have problems within their department. B. It is presumed that these problems are caused by a lack of standards and/or a failure to meet existing standards. 4. Results and Conclusions A. The objectives of the majority of health-care facilities is to provide the best possible care at lowest possible price. The objectives of the foodservice department is to provide the best possible food at the most reasonable cost. B. Continuous education of foodservice directors in all facets of the industry are necessary so that each foodservice director has the capacity to establish and maintain, and in some cases surpass his or her own standards. C. Where no standards exist minimum requirements should be set to satisfy the objectives and goals of both the facility and foodservice department.