1 resultado para alien alterity
em Digital Commons at Florida International University
Filtro por publicador
- University of Cagliari UniCA Eprints (1)
- Aberdeen University (1)
- Abertay Research Collections - Abertay University’s repository (1)
- Aberystwyth University Repository - Reino Unido (2)
- Academic Research Repository at Institute of Developing Economies (2)
- Acceda, el repositorio institucional de la Universidad de Las Palmas de Gran Canaria. España (1)
- Adam Mickiewicz University Repository (2)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (1)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (2)
- Andina Digital - Repositorio UASB-Digital - Universidade Andina Simón Bolívar (3)
- Aquatic Commons (10)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (4)
- Archive of European Integration (1)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (3)
- Aston University Research Archive (4)
- Avian Conservation and Ecology - Eletronic Cientific Hournal - Écologie et conservation des oiseaux: (1)
- Biblioteca de Teses e Dissertações da USP (1)
- Biblioteca Digital | Sistema Integrado de Documentación | UNCuyo - UNCUYO. UNIVERSIDAD NACIONAL DE CUYO. (3)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (9)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (3)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (30)
- Bibloteca do Senado Federal do Brasil (1)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (43)
- Brock University, Canada (1)
- CaltechTHESIS (1)
- CentAUR: Central Archive University of Reading - UK (20)
- Center for Jewish History Digital Collections (2)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (13)
- CORA - Cork Open Research Archive - University College Cork - Ireland (5)
- Corvinus Research Archive - The institutional repository for the Corvinus University of Budapest (2)
- Deakin Research Online - Australia (58)
- Digital Archives@Colby (2)
- Digital Commons at Florida International University (1)
- Digital Peer Publishing (2)
- Digitale Sammlungen - Goethe-Universität Frankfurt am Main (2)
- DRUM (Digital Repository at the University of Maryland) (1)
- Duke University (3)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (11)
- Fachlicher Dokumentenserver Paedagogik/Erziehungswissenschaften (4)
- Gallica, Bibliotheque Numerique - Bibliothèque nationale de France (French National Library) (BnF), France (1)
- Greenwich Academic Literature Archive - UK (1)
- Helda - Digital Repository of University of Helsinki (10)
- Indian Institute of Science - Bangalore - Índia (4)
- Instituto Politécnico do Porto, Portugal (1)
- Lume - Repositório Digital da Universidade Federal do Rio Grande do Sul (1)
- Memoria Académica - FaHCE, UNLP - Argentina (31)
- Ministerio de Cultura, Spain (1)
- National Center for Biotechnology Information - NCBI (5)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (7)
- Portal de Revistas Científicas Complutenses - Espanha (14)
- Publishing Network for Geoscientific & Environmental Data (16)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (38)
- Queensland University of Technology - ePrints Archive (24)
- RDBU - Repositório Digital da Biblioteca da Unisinos (5)
- ReCiL - Repositório Científico Lusófona - Grupo Lusófona, Portugal (1)
- Repositorio Académico de la Universidad Nacional de Costa Rica (1)
- Repositório Científico da Universidade de Évora - Portugal (1)
- Repositório Científico do Instituto Politécnico de Lisboa - Portugal (2)
- Repositório digital da Fundação Getúlio Vargas - FGV (5)
- Repositório Institucional da Universidade de Aveiro - Portugal (2)
- Repositório Institucional da Universidade Estadual de São Paulo - UNESP (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (53)
- Research Open Access Repository of the University of East London. (1)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (1)
- SAPIENTIA - Universidade do Algarve - Portugal (1)
- Universidad de Alicante (1)
- Universidad del Rosario, Colombia (4)
- Universidad Politécnica de Madrid (1)
- Universidade de Lisboa - Repositório Aberto (2)
- Universidade dos Açores - Portugal (2)
- Universidade Federal de Uberlândia (2)
- Universidade Federal do Pará (12)
- Universidade Federal do Rio Grande do Norte (UFRN) (18)
- Universidade Metodista de São Paulo (8)
- Université de Lausanne, Switzerland (1)
- Université de Montréal (1)
- Université de Montréal, Canada (20)
- Université Laval Mémoires et thèses électroniques (1)
- University of Michigan (25)
- University of Queensland eSpace - Australia (5)
- University of Washington (2)
- WestminsterResearch - UK (1)
- Worcester Research and Publications - Worcester Research and Publications - UK (2)
Resumo:
Background Post transcriptional gene silencing (PTGS) is a mechanism harnessed by plant biologists to knock down gene expression. siRNAs contribute to PTGS that are synthesized from mRNAs or viral RNAs and function to guide cellular endoribonucleases to target mRNAs for degradation. Plant biologists have employed electroporation to deliver artificial siRNAs to plant protoplasts to study gene expression mechanisms at the single cell level. One drawback of electroporation is the extensive loss of viable protoplasts that occurs as a result of the transfection technology. Results We employed fluorescent conjugated polymer nanoparticles (CPNs) to deliver siRNAs and knockdown a target gene in plant protoplasts. CPNs are non toxic to protoplasts, having little impact on viability over a 72 h period. Microscopy and flow cytometry reveal that CPNs can penetrate protoplasts within 2 h of delivery. Cellular uptake of CPNs/siRNA complexes were easily monitored using epifluorescence microscopy. We also demonstrate that CPNs can deliver siRNAs targeting specific genes in the cellulose biosynthesis pathway (NtCesA-1a and NtCesA-1b). Conclusions While prior work showed that NtCesA-1 is a factor involved in cell wall synthesis in whole plants, we demonstrate that the same gene plays an essential role in cell wall regeneration in isolated protoplasts. Cell wall biosynthesis is central to cell elongation, plant growth and development. The experiments presented here shows that NtCesA is also a factor in cell viability. We show that CPNs are valuable vehicles for delivering siRNAs to plant protoplasts to study vital cellular pathways at the single cell level.