Hydrogel formulation for enhanced regeneration in cell transplantation

Myocardial cell transplantation can compensate for the loss of necrotic cardiomyocytes. The objective of this research study was to reformulate the hydrogel with concentrations of growth factors, such as Leukemia Inhibitory Factor (LIF), Hepatocyte Growth Factor (HGF), and Interleukin-6 (IL-6). A controlled delivery system of PEO-PPO-PEO was formulated for release of a single growth factor and of multiple growth factors. Cytotoxicity and proliferation assay for single growth factors starting with 4000 skeletal myoblasts yielded their highest proliferation at 4 days with HGF (25,500 cells) and LIF (42,000 cells), while IL-6 (115,000 cells) generated its highest proliferation at 5 days. Combination of LIF and IL-6 resulted in highest proliferation at day 2 (220,000 cells), HGF and LIF (108,000 cells), and HGF and IL-6 (80,000 cells) both at 5 days. Viability at 37°C was maintained during the five days at 98-99%. The formulation was successful in myotube formation while maintaining a high purity of myoblasts in culture. The new formulation induced controlled release of growth factors and skeletal myoblasts delivery under favorable conditions while increasing the proliferation of myoblasts.

The creation of Huey P. Newton

The purpose of this study was to examine the creation of Huey P. Newton, founder of the Black Panther Party in 1966. I argued that Huey P. Newton was a creation of several elements: the black ghetto of Oakland; the rise of Black Power and the death of non-violence in the civil rights movement; the New Left and its factions; and, the Black Panther Party through the "Free Huey" campaign. The "Free Huey" campaign that arose from Newton's imprisonment in 1968, constructed an iconic image of Newton that he inherited on his release in 1970. This study will contextualize Newton and refute the claims of Hugh Pearson, author of the 1994, The Shadow of the Panther, who deemed Newton as a common criminal, not worthy of historical debate.