Características de virulência, resistência e diversidade genética de sorovares de Salmonella com impacto na saúde pública, isolados de frangos de corte no Brasil /

Salmonella Enteritidis, S. Typhimurium and S. Infantis are often associated with cases of human infections worldwide and is transmitted through consumption of contaminated food, particularly those of animal origin, especially chicken meat. This thesis was fractionated into three chapters, the first one relating to general considerations about the topics discussed in the following chapters. The second chapter aimed to evaluate virulence characteristics, antimicrobial resistance and the genetic similarity of 51 strains of S. Infantis isolated in samples of poultry origin from an industry located in the state of São Paulo, Brazil, during the 2009 to 2010 period. The third chapter aimed to analyze 111 strains of S. Enteritidis, 45 of Salmonella Typhimurium and 31 of Salmonella Typhimurium monophasic variant I 4, [5], 12:i:- isolated from chicken carcasses in different brazilian slaughterhouses from 2009 to 2011, and to estimate the risk to human health, based on the presence of virulence genes and antimicrobial resistance, correlating to the pathogenicity profiles (antimicrobial resistance and presence of virulence and resistance genes) with the genetic profile (ribogroup) of the isolates. To evaluate the antimicrobial susceptibility was performed the disk diffusion test for all serotypes of Salmonella, and exclusively to S. Enteritidis and S. Typhimurium, was also verified the minimum inhibitory concentration for ciprofloxacin and ceftazidime antibiotics. The presence of virulence genes invA (invasion), lpfA (fimbriae-adhesion), agfA (fimbriae-biofilm) and sefA (fimbriae-adhesion) were evaluated by PCR. The strains that showed resistance to antibiotics of β-lactams class were evaluated for the presence of resistance genes blaTEM, blaSHV, blaCTX-M and blaAmpC. For resistant strains to quinolones and fluoroquinolones antibiotics classes were searched the qnrA and qnrS genes. The phylogenetic relationship among the isolates was determined by RAPD method for S. Infantis strains, and by ribotyping technique to S. Enteritidis and S. Typhimurium.

Do reconhecimento à efetividade do direito fundamental à água: diálogos entre Nicarágua e Brasil

La humanidad está en un periodo de transición paradigmática, donde aún no existe acuerdo sobre en cual periodo nos encuadramos, sí modernidad o posmodernidad, pero el debate de ahí surgido se revela útil y transversal a los dilemas que enfrenta hoy la sociedad. Uno de esos dilemas tiene que ver con el reconocimiento del agua como derecho fundamental, ciertamente la era de la información ha permitido avanzar en armar el rompecabezas de la crisis hídrica mundial y sus riesgos, lo que ha develado una realidad importante, el acceso al agua tanto en naciones económicamente desarrolladas como en las menos desarrolladas constituye aun una aspiración, son varias las causas de esto, siendo una de las más comunes la atribuida al cambio climático, sin embargo, al profundizar en la materia surgen otras causas como la falta de voluntad política a nivel nacional, los intereses económicos que dan al agua tratamiento de mercancía y a nivel internacional la existencia de luces y sombras en el campo de los derechos humanos, estas constituyen las piezas del mencionado rompecabezas, aun por armar. Mientras tanto el Derecho tiene aquí un desafío de significativa importancia, adaptarse a fin de responder adecuadamente a las nuevas realidades: riesgos de diversas índoles. Esta investigación tiene como objetivo defender la existencia del derecho fundamental al agua en el ordenamiento jurídico brasileño y nicaragüense, comparando instrumentos que puedan asegurar su efectividad. Para la realización de este trabajo se utilizó un abordaje inductivo-comparativo, fuentes bibliográficas brasileñas, nicaragüenses y de países con experiencias relevantes para la comprensión del problema y que pudieran aportar propuestas de cara al reconocimiento y efectivación del derecho fundamental al agua. Este trabajo encontró que existen ambigüedades importantes en el campo del Derecho Humano al Agua motivadas por el trabajo de agencias que defienden el derecho pero a la vez establecen coordinaciones y normativas con quienes impulsan su privatización, fue posible presentar elementos que apoyan la fundamentalidad del derecho al agua desde un punto de vista material y de vinculación con otros derechos fundamentales, pero preocupa en el caso de Brasil y Nicaragua el poco avance jurisprudencial de cara al reconocimiento y finalmente la atribución de la crisis hídrica en muchos casos a la incertidumbre climática, cuando uno de los principales desafíos se encuentra en la explotación comercial del agua.

Relação entre a cárie precoce da infância com o índice de Apgar e outros fatores de risco

The aim of this cross-sectional study was to investigate the association of early childhood caries (ECC) with the Apgar score (AS) and other variables related to the child (conditions at birth and medical history) and related to the child and parents and / or guardians and family (demographic, socioeconomic and behavioral). One hundred and twenty healthy children aged between 3-5 years-old treated by Pediatric Dentistry Area of Dentistry College of the Federal University of Uberlandia during 2015 were selected. To obtain qualitative and quantitative variables a questionnaire was applied as an interview to the parents and/or guardians. The 5-minute AS (interest exposure) was obtained through the record in the Child Health Handbook. To assess the prevalence of caries (clinical dependent variable), a single calibrated researcher conducted the clinical examination, according to the criteria of the World Health Organization. Caries experience was measured using the indexes dmft and dmfs. The children were classified into three groups, according to age and dmfs index: no caries (NC), with ECC and with severe early childhood caries (S-ECC). Data were tabulated and submitted to statistical analysis using the SPSS software (IBM, Inc, Chicago, Illinois, USA) 17th version. Three logistics models were carried out having the following classifications: NC and ECC, NC and S-ECC, ECC and S-ECC (p<0.05). The overall ECC prevalence, considering children with ECC and S-ECC, was 55,8% (n= 67). The AS was not a statistically significant variable. The child’s age, weaning age and recent hospitalization were variables associated with the ECC prevalence. The age of brush start and the educational level of the mother were variables associated with the S-ECC prevalence. Considering the ECC and the S-ECC groups, the child's age and the beginning of the use of fluoride toothpaste, recent hospitalization, the educational level of the mother and the father's income were associated with the S-ECC prevalence. Considering the methodology employed and the analysis of results, it was concluded that there was no association between the ECC with the AS in healthy children. However, an association was found of ECC and S-ECC with some variables related to birth and to medical history of the child (recent hospitalization), demographic (child’s age), socioeconomic (educational level of the mother and father's income) and behavioral (age of brush start, weaning age and use of fluoride toothpaste) related to children and to the parents and/or guardians.