Perfil sorológico contra Rickettsia spp e Leptospira spp e infestação de carrapatos em suínos mantidos sob diferentes sistemas de criação

The various types of pig farming, intensive and extensive, expose them to pig parasites but also to those from the environment of the breeding site. In this work was evaluated the exposure of bigs bred in technified farms, SISCAL (intensive breeding system in pens) and not technified (backyard) to leptospira, ticks and rickettsiae. Blood sera were analyzed to determine titers of antibodies anti-Leptospira by SAM technique and antibodies anti-rickettsial by IFA, pigs were inspected for ticks and in their breeding environment and surrounding areas (pastures and riparian vegetation), ticks were collected by the flannel dragging technique. In the farms of pigs 10.4% had anti-Leptospira antibodies, followed by SISCAL (8%) and backyard animals (2.5%). The serovars found were Bratislava, Pomona, serovar, Canicola and Icterohaemorrhagiae. Higher percentage of properties with pigs raised outdoors (SISCAL) had tick infested animals (20%) than those raised in backyard (6.7%), while commercial farms had no infested pigs nor infested breeding place. In both SISCAL and backyard pig breeding properties ticks were observed at the breeding site environment. Tick infestations were detected in areas surrounding pig breeding site in all three husbandry suystems. Ticks found were all Amblyomma scultpum nymphs or adults with the exception of one of Amblyomma parvum adult. In relation to anti-rickettsia serology to five Rickettsia species, 55.2% of pigs from commercial farms reacted to al least one species, backyard pigs reacted to 89.7% and all pigs of SISCAL showed anti-rickettsia titers. Consecutive tick sampling (June 2014 to February 2016) in SISCAL FAZU in Uberaba, showed the establishment A. sculptum ticks maintained by domestic pigs. These observations demonstrate the ability the pigs to maintain populations of A. sculptum at a favorable environment and may indicate a new trend in environmental infestations by this species of tick. Exposure to Leptospira and Rickettsia demonstrated the potential pigs exposure and transmission of important diseases in public health.

Características de virulência, resistência e diversidade genética de sorovares de Salmonella com impacto na saúde pública, isolados de frangos de corte no Brasil /

Salmonella Enteritidis, S. Typhimurium and S. Infantis are often associated with cases of human infections worldwide and is transmitted through consumption of contaminated food, particularly those of animal origin, especially chicken meat. This thesis was fractionated into three chapters, the first one relating to general considerations about the topics discussed in the following chapters. The second chapter aimed to evaluate virulence characteristics, antimicrobial resistance and the genetic similarity of 51 strains of S. Infantis isolated in samples of poultry origin from an industry located in the state of São Paulo, Brazil, during the 2009 to 2010 period. The third chapter aimed to analyze 111 strains of S. Enteritidis, 45 of Salmonella Typhimurium and 31 of Salmonella Typhimurium monophasic variant I 4, [5], 12:i:- isolated from chicken carcasses in different brazilian slaughterhouses from 2009 to 2011, and to estimate the risk to human health, based on the presence of virulence genes and antimicrobial resistance, correlating to the pathogenicity profiles (antimicrobial resistance and presence of virulence and resistance genes) with the genetic profile (ribogroup) of the isolates. To evaluate the antimicrobial susceptibility was performed the disk diffusion test for all serotypes of Salmonella, and exclusively to S. Enteritidis and S. Typhimurium, was also verified the minimum inhibitory concentration for ciprofloxacin and ceftazidime antibiotics. The presence of virulence genes invA (invasion), lpfA (fimbriae-adhesion), agfA (fimbriae-biofilm) and sefA (fimbriae-adhesion) were evaluated by PCR. The strains that showed resistance to antibiotics of β-lactams class were evaluated for the presence of resistance genes blaTEM, blaSHV, blaCTX-M and blaAmpC. For resistant strains to quinolones and fluoroquinolones antibiotics classes were searched the qnrA and qnrS genes. The phylogenetic relationship among the isolates was determined by RAPD method for S. Infantis strains, and by ribotyping technique to S. Enteritidis and S. Typhimurium.

O ensino de química na educação de jovens e adultos do ensino médio no município de Itumbiara-GO

For this study, a research was conducted in order to answer the question "What chemistry teaching has been developed in the Youth and Adult Education (EJA) ?". The research provides an overview of the object to the proposed changes, leading students to live with different realities and investigating the issue of contextualization based on the daily lives of these students related to the subject of chemistry. The methodology focuses ethnographic research of the case study, in which a case is studied in depth using the participant observation. In the survey data a mixed qualitative and quantitative approach was used. The work involved 6 schools that offer adult education high school; 6 directors of these schools; 6 coordinators who work in adult education; 6 Chemistry teachers and 123 students of the EJA, level high school, enrolled in the 1st, 2nd, 3rd and 4th periods. The first stage of the research consisted of questionnaires in schools where everyone involved responded closed and open questions applied to each specific group. In the second stage two schools were selected in order to conduct a deeper knowledge of adult education through practical activities of Chemistry and subsequent interview conducted in groups with students. Three teachers were also interviewed to enable a deepening of issues relating to EJA and Chemistry Teaching. The interviews were analyzed by the technique of Discursive Textual Analysis (ATD). The main issues addressed in the questionnaires and interviews were on the school structure, reasons that lead students to drop out or remained in adult education and those who make the stay, the view of those involved of the importance of chemistry discipline for students of EJA and how this should be offered. It is necessary that we need to promote changes in the chemistry class and its activities, respecting the experiences and experience already gained by the student during his life story. Another factor to be highlighted is the need for ongoing training of teachers working in adult education. Note that your continued education is given more by the experience and the ways in which they try to overcome adverse situations. The Chemistry subject taught is not agree with the principles of EJA and practiced curriculum is just an adaptation or content reduction from the regular curriculum. The improvement in chemistry teaching of EJA will take place through a dialogue between those involved in the process, clearer educational policies and willingness to implement change. Thus the teaching of chemistry contribute to the students of the EJA are actually scientifically literate and integrated into society.