Efeito da oxigenação hiperbárica no reparo ósseo de ratos diabéticos

The chronic state of hyperglycemia due to diabetes mellitus affects multiples organs impairing life quality. In bone, diabetes alters strength and mineral density and also suppresses the osteoblast activity, leading to an unbalanced bone healing process. Hyperbaric oxygen therapy (HBO) is suggested as an adjuvant treatment to accelerate bone repair. This study evaluated the effects of HBO in the number of mast cells and in new bone formation at the initial stage of bone repair in normoglycemic and diabetic rats. It was hypothesized that HBO treatment may improve bone repair in diabetic bone. The rats were equally divided in four groups: Control (C); Control + HBO (CH); Diabetes (D) and Diabetes + HBO (DH). Diabetes was induced by streptozotocin (65mg/kg) and femoral bone defects were created thirty days after diabetes induction in all groups. HBO initiated immediately after surgery procedure and was performed daily, for 7 days, in the CH e DH groups. Seven days after surgery, all animals were euthanized. The femur diaphyses were removed, fixated, decalcified and processed for paraffin embedding. The semi-serial histological sections obtained were stained with Hematoxylin-Eosin (HE), Mallory Trichrome and Toluidine Blue. The qualitative analysis was conducted in the histology slides stained with HE, where it was evaluated the morphological aspects of bone repair in the lesion area, observing the presence of clot, inflammatory cells, granulation tissue, type of bone tissue, morphology of bone cells, and thickness and organization of bone trabeculae. In the slides stained with Mallory Trichrome and Toluidine Blue were evaluated the percentage of new bone formation and number of mast cells, respectively. The qualitative analysis showed that the CH group presented a more advanced stage of bone repair compared to the C group, showing thicker trabeculae and greater bone filling of the lesion area. In D and DH group, the lesion area was partially filled with new bone formation tissue and presented thinner trabeculae and fewer areas associated to osteoclasts compared to control group. The histomorphometric analysis showed a significant improvement in new bone formation (p<0.001) comparing CH (38.08 ± 4.05) and C (32.05 ± 5.51); C and D (24.62 ± 2.28 and CH and DH (27.14 ± 4.21) groups. In the normoglycemic rats there was a significant increasing in the number of mast cells (p<0.05) comparing C (8.06 ± 5.15) and CH (21.06 ± 4.91) groups. In conclusion, this study showed that diabetes impaired bone repair and HBO was only able to increase new bone formation and the number of mast cells in the normoglycemic animals.

Cidades saudáveis e qualidade de vida em Uberlândia (MG): aportes metodológicos para um processo em construção

The current study includes theoretical and methodological reflections on the quality of life in the city of Uberlândia, Minas Gerais. It started from the thought that the quality of life is multifactorial and is permanently under construction and the main objective of analyzing it as one of the componets of Healthy Cities's moviment. The theoretical research focused on the concepts of healthy cities, quality of life, health, sustainability, well-being, happiness, indexes and indicators. From the use of multiple search strategies, documentary and on field of quantitative and qualitative character, this research of exploratory descriptive nature can offers a contribution to the studies on the quality of life in cities. It is proposed that the studies startes to work with some concept, like some notions os life quality adequated for some paticular reality, whose notions can approach concepts already established as health. This step is important on the exploratory researches. The studies may include aspects of objective analysis, subjective or both. The objective dimension, which is most common approach, are traditionally considered variables and indicators related to: the urban infrastructure (health, education, leisure, security, mobility), dwelling (quantitative and qualitative dwlling deficit), the urban structure (density and mix uses), socioeconomic characteristics (age, income, education), urban infrastructure (sanitation, communication), governance (social mobilization and participation). To focus on the subjective dimension, most recent and unusual, it is proposed to consider the (dis)satisfaction, the personal assessment in relation to the objective aspects. In conclusion, being intrinsically related to the health, the quality of life also has a number of determinants, and the ideal of the reach of quality of life depends on the action of all citizens based on the recognition of networks and territories, in a interescalar perspective and intersectoral. Therefore, emphasis in given on the potential of tools, such as the observatories, to monitor and intervent in reality, aiming in a building process of healthy cities.