Végtelen sokszor ismételt kiszerződés (Infinitely repeated outsourcing)

A cikk a kormányzat és a vállalat közti végtelen sokszor ismételt kiszerződésekkel foglalkozik. Vizsgálati eszköztárát a nem teljes szerződések elméletéből kölcsönzi. A szakirodalmat felhasználva megmutatja, hogy létezik olyan fenyegetési stratégia, köznapi szóhasználattal az adott szabályok esetén mindkét fél számára saját érdekében betartandó stratégia, ahol mind a vállalat, mind a kormányzat beruházásának értéke a társadalmilag optimális nagyság. Bebizonyítja, hogy annál valószínűbb, hogy mind a kormányzat, mind a vállalat a társadalmilag optimális beruházási szintet valósítja meg, minél nagyobb annak az esélye, hogy az adott vállalat kapja meg a következő periódusban is az ismétlődő munkafeladatot. Felhívja a figyelmet a szabályozó felelősségére az újabb szerződés valószínűségének indirekt meghatározásában. ____ The paper analyses the in¯nitely repeated contracts between the government and a firm. Methods from the incomplete contract theory are used for this analysis. Based on the literature, it is shown that there exist a trigger strategy, when both the government and the firm deploy the socially optimal amount of investment. The paper proves that the greater the chance that an excellently accomplished firm receives a new contract the higher is the probability that both actors' investments are on the socially optimal level. The regulator has a great liability in establishing indirectly the chance of receiving a new contract opportunity.

Targeting dsRNA-specific single-chain Fv antibody fragments to different cellular locations in Nicotiana tabacum L.

Expression of antibodies or antibody fragments in plants is a useful tool for producing active antibody derivatives for diagnostic or pharmaceutical purposes as well as for immunomodulation. We investigated the effect of cellular expression site on the stability and yield of double-stranded RNA (dsRNA)-specific single-chain Fv-fragments (scFv) in transgenic tobacco. Two antibodies (J2 and P6) belonging to the V23(J558) heavy chain variable gene family but differing in the light chain variable domain were used. scFvs were targeted to the cytoplasm – with or without anchoring them in the plasma membrane –, into the endoplasmic reticulum (ER) and to the apoplast. Although high mRNA concentrations were detected in all cases, scFv proteins accumulated only when scFvs were made ER-resident by appropriate signal sequences. When the ER retention signal was removed to allow scFv-secretion to the apoplast, no scFv-proteins were detected. Despite the strong homology of the VH-sequences of J2 and P6 antibodies, only P6 provided a stable scFv scaffold for intracytoplasmic expression. J2-scFv could not be stabilised neither by adding a C-terminal stabilisation signal nor by anchoring the protein at the cytoplasmic side of the plasma membrane (PM). It was found that dsRNA-specific J2-scFvs are active in vivo and enhance Potato Virus Y induced symptoms in infected tobacco. This is the first report describing the expression and biological effect of RNA-specific antibodies in plants.