1 resultado para Amplify-and-Forward
em Corvinus Research Archive - The institutional repository for the Corvinus University of Budapest
Filtro por publicador
- KUPS-Datenbank - Universität zu Köln - Kölner UniversitätsPublikationsServer (1)
- Repository Napier (2)
- ABACUS. Repositorio de Producción Científica - Universidad Europea (1)
- Aberdeen University (1)
- Aberystwyth University Repository - Reino Unido (2)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (2)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (2)
- Aquatic Commons (3)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (2)
- Archimer: Archive de l'Institut francais de recherche pour l'exploitation de la mer (1)
- Archive of European Integration (17)
- Aston University Research Archive (28)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (7)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (4)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (16)
- Boston University Digital Common (4)
- Bucknell University Digital Commons - Pensilvania - USA (1)
- Bulgarian Digital Mathematics Library at IMI-BAS (1)
- CaltechTHESIS (7)
- Cambridge University Engineering Department Publications Database (21)
- CentAUR: Central Archive University of Reading - UK (45)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (20)
- Cochin University of Science & Technology (CUSAT), India (2)
- Coffee Science - Universidade Federal de Lavras (1)
- Comissão Econômica para a América Latina e o Caribe (CEPAL) (5)
- Corvinus Research Archive - The institutional repository for the Corvinus University of Budapest (1)
- Deakin Research Online - Australia (47)
- Department of Computer Science E-Repository - King's College London, Strand, London (2)
- Digital Archives@Colby (2)
- Digital Commons at Florida International University (3)
- DigitalCommons@The Texas Medical Center (2)
- DigitalCommons@University of Nebraska - Lincoln (1)
- Doria (National Library of Finland DSpace Services) - National Library of Finland, Finland (1)
- DRUM (Digital Repository at the University of Maryland) (2)
- Duke University (1)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (14)
- FUNDAJ - Fundação Joaquim Nabuco (1)
- Greenwich Academic Literature Archive - UK (4)
- Helda - Digital Repository of University of Helsinki (16)
- Indian Institute of Science - Bangalore - Índia (95)
- Instituto de Engenharia Nuclear, Brazil - Carpe dIEN (1)
- Instituto Politécnico de Viseu (1)
- Instituto Politécnico do Porto, Portugal (4)
- National Center for Biotechnology Information - NCBI (2)
- Nottingham eTheses (1)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (2)
- Publishing Network for Geoscientific & Environmental Data (2)
- QSpace: Queen's University - Canada (2)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (96)
- Queensland University of Technology - ePrints Archive (324)
- RCAAP - Repositório Científico de Acesso Aberto de Portugal (1)
- ReCiL - Repositório Científico Lusófona - Grupo Lusófona, Portugal (1)
- Repositório Científico do Instituto Politécnico de Lisboa - Portugal (1)
- Repositório digital da Fundação Getúlio Vargas - FGV (12)
- Repositório Institucional da Universidade de Aveiro - Portugal (3)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (43)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (2)
- SAPIENTIA - Universidade do Algarve - Portugal (1)
- Scielo España (1)
- Universidad de Alicante (2)
- Universidad del Rosario, Colombia (1)
- Universidad Politécnica de Madrid (9)
- Universidade do Algarve (1)
- Universidade Federal do Pará (2)
- Universidade Federal do Rio Grande do Norte (UFRN) (1)
- Universidade Técnica de Lisboa (1)
- Université de Montréal, Canada (3)
- University of Canberra Research Repository - Australia (1)
- University of Michigan (17)
- University of Queensland eSpace - Australia (12)
- University of Southampton, United Kingdom (1)
- University of Washington (4)
- WestminsterResearch - UK (3)
- Worcester Research and Publications - Worcester Research and Publications - UK (1)
Resumo:
Inverted repeat gene structures designed for silencing functional genes have been widely used both in academic and applied research. The correct orientations of such structures are usually validated with restriction analysis and/or sequencing. We speculated that the inverted repeat nature of such constructs can be shown by a simple PCR reaction with a single forward primer. To test this hypothesis five different constructs were established from grapevine sequences in a hairpin-intron style silencing system. We were able to amplify the appropriate products in each case. Thus a forward-primed PCR alone may be sufficient to prove the inverted repeat nature of the desired constructs.