PLGA microspheres for the delivery of a novel subunit TB vaccine

Biodegradable poly(dl-lactide-co-glycolide) microspheres were prepared using a modified double emulsion solvent evaporation method for the delivery of the subunit tuberculosis vaccine (Ag85B-ESAT-6), a fusion protein of the immunodominant antigens 6-kDa early secretory antigenic target (ESAT-6) and antigen 85B (Ag85B). Addition of the cationic lipid dimethyl dioctadecylammonium bromide (DDA) and the immunostimulatory trehalose 6,6'-dibehenate (TDB), either separately or in combination, was investigated for the effect on particle size and distribution, antigen entrapment efficiency, in vitro release profiles and in vivo performance. Optimised formulation parameters yielded microspheres within the desired sub-10 mu m range (1.50 +/- 0.13 mu m), whilst exhibiting a high antigen entrapment efficiency (95 +/- 1.2%) and prolonged release profiles. Although the microsphere formulations induced a cell-mediated immune response and raised specific antibodies after immunisation, this was inferior to the levels achieved with liposomes composed of the same adjuvants (DDA-TDB), demonstrating that liposomes are more effective vaccine delivery systems compared with microspheres.

W Primary Care Trust v TB & Ors [2009] EWHC 1737

The primary issue in this case related to TB’s clear and expressed desire to leave V, in order that she might be admitted to an NHS hospital for treatment of what she believed to be a physical, as opposed to a psychological, condition...

73.7 Tb/s (96 x 3 x 256-Gb/s) mode-division-multiplexed DP-16QAM transmission with inline MM-EDFA

Transmission of a 73.7 Tb/s (96x3x256-Gb/s) DP-16QAM mode-division-multiplexed signal over 119km of few-mode fiber transmission line incorporating an inline multi mode EDFA and a phase plate based mode (de-)multiplexer is demonstrated. Data-aided 6x6 MIMO digital signal processing was used to demodulate the signal. The total demonstrated net capacity, taking into account 20% of FEC-overhead and 7.5% additional overhead (Ethernet and training sequences), is 57.6 Tb/s, corresponding to a spectral efficiency of 12 bits/s/Hz.

Impact of Raman amplification on a 2 Tb/s coherent WDM system

The impact of hybrid erbium-doped fiber amplifier (EDFA)/Raman amplification on a spectrally efficient coherent-wavelength-division-multiplexed (CoWDM) optical communication system is experimentally studied and modeled. Simulations suggested that 23-dB Raman gain over an unrepeatered span of 124 km single-mode fiber would allow a decrease of the mean input power of ~6 dB for a fixed bit-error rate (BER). Experimentally we demonstrated 1.2-dB Q-factor improvement for a 2-Tb/s seven-band CoWDM with backward Raman amplification. The system delivered an optical signal-to-noise ratio of 35 dB at the output of the receiver preamplifier providing a worst-case BER of 2 × 10 -6 over 49 subcarriers at 42.8 Gbaud, leaving a system margin (in terms of Q -factor) of ~4 dB from the forward-error correction threshold.

1-Tb/s DWDM long-haul transmission employing a fiber optical parametric amplifier

In this letter, we report the performance of a fiber optical parametric amplifier (OPA) when used as a source or intermediate node amplifier in a dense wavelength-division-multiplexed (DWDM) long-haul transmission testbed with 26 DWDM channels modulated at 43.7-Gb/s return-to-zero differential phase-shift keying. In both scenarios, we demonstrate similar performance to an erbium-doped fiber amplifier. This shows the OPAs compatibility with high-capacity (>1 Tb/s) long-haul communication systems.

Fiber optical parametric amplifier performance in a 1-Tb/s DWDM communication system

We have reduced signal-signal four-wave mixing crosstalk in a fiber optical parametric amplifier (OPA) by using a short nonlinear fiber for the gain medium and a high-power pump. This allowed us to obtain less than 1 dB penalty for amplification of 26 dense wavelength-division multiplexed (WDM) channels modulated at 43.7Gb/s return to zero-differential phase-shift keying, with the OPA placed between transmitter and receiver. We then used the same OPA in several different roles for a long-haul transmission system. We did not insert the OPA within the loop, but investigated this role indirectly by using equivalent results for small numbers of loop recirculations. We found that standard erbium-doped fiber amplifiers currently hold an advantage over this OPA, which becomes negligible for long distances. This paper shows that at this time OPAs can handle amplification of WDM traffic in excess of 1 Tb/s with little degradation. It also indicates that with further improvements, fiber OPAs could be a contender for wideband amplification in future optical communication networks.

1-Tb/s DWDM amplification in a fiber optical parametric amplifier

We report less than 1-dB cross-talk penalty for 26 DWDM channels modulated at 43.7 Gb/s RZ-DPSK when amplified by a fiber optical parametric amplifier showing compatibility with high-capacity (> 1 Tb/s) communication systems. © 2010 Optical Society of America.

1-Tb/s DWDM amplification in a fiber optical parametric amplifier

We report less than 1-dB cross-talk penalty for 26 DWDM channels modulated at 43.7 Gb/s RZ-DPSK when amplified by a fiber optical parametric amplifier showing compatibility with high-capacity (> 1 Tb/s) communication systems. © 2010 Optical Society of America.

1-Tb/s DWDM long-haul transmission employing a fiber optical parametric amplifier

In this letter, we report the performance of a fiber optical parametric amplifier (OPA) when used as a source or intermediate node amplifier in a dense wavelength-division-multiplexed (DWDM) long-haul transmission testbed with 26 DWDM channels modulated at 43.7-Gb/s return-to-zero differential phase-shift keying. In both scenarios, we demonstrate similar performance to an erbium-doped fiber amplifier. This shows the OPAs compatibility with high-capacity (>1 Tb/s) long-haul communication systems.

Digital fiber nonlinearity compensation:toward 1-Tb/s transport

The world is connected by a core network of long-haul optical communication systems that link countries and continents, enabling long-distance phone calls, data-center communications, and the Internet. The demands on information rates have been constantly driven up by applications such as online gaming, high-definition video, and cloud computing. All over the world, end-user connection speeds are being increased by replacing conventional digital subscriber line (DSL) and asymmetric DSL (ADSL) with fiber to the home. Clearly, the capacity of the core network must also increase proportionally. © 1991-2012 IEEE.

73.7 Tb/s (96X3x256-Gb/s) mode-division-multiplexed DP-16QAM transmission with inline MM-EDFA

We show transmission of a 73.7 Tb/s (96x3x256-Gb/s) DP-16QAM modedivision- multiplexed signal over 119km of few-mode fiber with inline multi-mode EDFA, using 6x6 MIMO digital signal processing. The total demonstrated net capacity is 57.6 Tb/s (SE 12 bits/s/Hz). © 2012 OSA.

73.7 Tb/s (96 x 3 x 256-Gb/s) mode-divisionmultiplexed DP-16QAM transmission with inline MM-EDFA

Transmission of a 73.7 Tb/s (96x3x256-Gb/s) DP-16QAM mode-division- multiplexed signal over 119km of few-mode fiber transmission line incorporating an inline multi mode EDFA and a phase plate based mode (de-)multiplexer is demonstrated. Data-aided 6x6 MIMO digital signal processing was used to demodulate the signal. The total demonstrated net capacity, taking into account 20% of FEC-overhead and 7.5% additional overhead (Ethernet and training sequences), is 57.6 Tb/s, corresponding to a spectral efficiency of 12 bits/s/Hz. © 2012 Optical Society of America.

4 Tb/s transmission reach enhancement using 10 × 400 Gb/s super-channels and polarization insensitive dual band optical phase conjugation

In this paper, we experimentally demonstrate the benefit of polarization insensitive dual-band optical phase conjugation for up to ten 400 Gb/s optical super-channels using a Raman amplified transmission link with a realistic span length of 75 km. We demonstrate that the resultant increase in transmission distance may be predicted analytically if the detrimental impacts of power asymmetry and polarization mode dispersion are taken into account.

4 Tb/s transmission reach enhancement using 10 × 400 Gb/s super-channels and polarization insensitive dual band optical phase conjugation

In this paper, we experimentally demonstrate the benefit of polarization insensitive dual-band optical phase conjugation for up to ten 400 Gb/s optical super-channels using a Raman amplified transmission link with a realistic span length of 75 km. We demonstrate that the resultant increase in transmission distance may be predicted analytically if the detrimental impacts of power asymmetry and polarization mode dispersion are taken into account.

Cascaded all-optical sub-channel add/drop multiplexing from a 1-Tb/s super-channel having 2-GHz guard-bands

We demonstrate cascaded 100-Gb/s sub-channel add/drop from a 1-Tb/s multi-band OFDM super-channel having 2-GHz inter-sub-channel guard-bands within a recirculating loop via a hierarchical ROADM using high-resolution filters, showcasing 1000-km transmission reach and five ROADM node passages for the add/drop sub-channel when hybrid Raman-EDFA is implemented.