Une simple amélioration ou une modernisation radicale? La réforme de l'administration de la Commission Européenne

If the need for change and improvement in the Commission’s ways of working became evident as of 1979, the reform process began with the Santer Commission. Although the public management reform seems to focus on reducing personnel and expenses, it goes further in the sense of modernization: budgetary reforms aim at budgeting results and performance; emphasis is put on individual responsibility and evaluation and on a more flexible approach to personnel management, strategic planning, and transfer of authority.

Dispersal of soredia from individual soralia of the lichen Hypogymnia physodes (L.) Nyl. in a simple wind tunnel

Dispersal of soredia from individual soralia of the lichen Hypogymnia physodes (L.) Nyl. was studied using a simple wind tunnel constructed in the field. Individual lobes with terminal soralia were placed in the wind tunnel on the adhesive surface of dust particle collectors. Air currents produced by a fan were directed over the surface of the lobes. The majority of soredia were deposited within 5 cm of the source soralium but some soredia were dispersed to at least 80 cm at a wind speed of 6 m s-1. Variation in wind speed had no statistically significant effect on the total number of soredial clusters deposited averaged over soralia but the mean size of cluster and the distance dispersed were greater at higher wind speeds. The number of soredia deposited was dependent on the orientation of the soralium to the air currents. More soredia were deposited with the soralium facing the fan at a wind speed of 9 m s-1. Moisture in the form of a fine mist reduced substantially the number of soredia deposited at a wind speed of 6 m s-1 but had no effect on the mean number of soredia per cluster or on the mean distance dispersed. The data suggest: (1) that wind dispersal from an individual soralium is influenced by wind speed, the location of the soralium on the thallus and the level of moisture and (2) that air currents directed over the surfaces of thalli located on the upper branches of trees would effectively disperse soredia of H. physodes vertically and horizontally within a tree canopy. © 1994.

Le présent suffit-il à faire l'histoire?

Our study discusses the ability of the présent historique, a tense « plus abstrait et donc plus difficile à comprendre » [more abstract and therefore more difficult to understand] (Béguin 1998, p. 36), to replace the passé simple (PS) in historical narration. It is based on a diachronic corpus of extracts taken from history books on the French Revolution, ranging from the beginning of the 19th century to the end of the 20th century. Firstly, we justify the primacy of the passé simple for historical narrative, while considering the wider areas of tense and aspect. After a description of the corpus and a summary of the hypotheses under study, we present our quantitative results with the aim of assessing the use of the présent historique in the corpus in comparison with other tenses. Finally, we study our corpus from a qualitative perspective and we try to conclude upon our initial hypotheses. © Revue Romane.

Il était une fois un match de foot:L'événement sportif comme objet de narration

L’analyse des temps de la narration n’est pas chose nouvelle. Souvent, ce sont les narrations orales ‘de tous les jours’ qui ont préoccupé les linguistes (Labov & Waletzky 1967, Bres 1994). Mais qu’en est-il de l’usage des temps dans les narrations écrites telles qu’on les trouve dans la presse sportive? On se fondera ici sur un corpus d’articles du 1er juillet 2002, tirés de la presse francophone (parisienne, régionale, belge et algérienne) et consacrés à la finale de la Coupe du Monde de football (Labeau 2002b). A quels temps a-t-on recours pour narrer ces exploits sportifs ? Le passé simple a-t-il toujours sa place dans ce genre « épique », ou s’est-il vu supplanter par d’autres formes telles le passé composé, le présent ou l’imparfait de narration… ? (Engel 1990) Nous allons nous concentrer sur deux aspects de l’emploi des temps. D’une part, la presse sportive constitue-t-elle un sous-genre distinct ? D’autre part, des différences régionales apparaissent-elles ? Nous tenterons de voir si les approches textuelles se reflètent dans l’emploi des temps des narrations étudiées. Termes clefs Narration - presse sportive – temps - presse régionale – presse francophone - genre

Nouveaux développements de l'imparfait

The uses of the French imperfect have undergone a vast extension throughout the history of the French language. It has become prototypical in contexts such as description where old French used simple past. The prototypical uses have resulted in a large number of stylistic uses: hypothesis, free indirect speech, and the so-called forain, hypocoristique, ludique and narratif. The wide range of uses raises the question of the semantic unity of that form in synchrony as well as in diachrony. Here are gathered for the first time discussions of emergent uses of the imperfect, that are tackled through a variety of approaches (Damourette and Pichon’s, Wilmet’s or Guillaume’s systems, logic semantics or relevance theory). This volume will be of interest for theoreticians interested in linguistic meaning and applied linguists concerned with its organization. Le tiroir de l'imparfait a subi une extension considérable de ses usages à travers l'histoire du français. Il est devenu prototypique dans des contextes comme la description où l'ancienne langue utilisait le passé simple. Ces emplois prototypiques ont donné lieu à un large ensemble d'usages stylistiques. : hypothèse, discours indirect libre, imparfait de politesse, forain, hypocoristique, ludique et narratif. La diversité des usages pose la question de l'unité sémantique de la forme, non seulement en synchronie mais aussi dans les cadres de son évolution diachronique et de son acquisition. Ce problème est appréhendé à travers les analyses de Damourette et Pichon et de Wilmet, par le guillaumisme, la sémantique logique et la pragmatique de la pertinence. Ces cadres permettent d'identifier les paramètres en jeu dans chaque emploi, et les modes de leur application à une problématique sémantique particulièrement délicate. Le présent ouvrage saura donc intéresser autant les théoriciens du sens linguistique que les praticiens concernés par son organisation.

A simple, sensitive and selective quantum-dot-based western blot method for the simultaneous detection of multiple targets from cell lysates

Quantum dots (Qdots) are fluorescent nanoparticles that have great potential as detection agents in biological applications. Their optical properties, including photostability and narrow, symmetrical emission bands with large Stokes shifts, and the potential for multiplexing of many different colours, give them significant advantages over traditionally used fluorescent dyes. Here, we report the straightforward generation of stable, covalent quantum dot-protein A/G bioconjugates that will be able to bind to almost any IgG antibody, and therefore can be used in many applications. An additional advantage is that the requirement for a secondary antibody is removed, simplifying experimental design. To demonstrate their use, we show their application in multiplexed western blotting. The sensitivity of Qdot conjugates is found to be superior to fluorescent dyes, and comparable to, or potentially better than, enhanced chemiluminescence. We show a true biological validation using a four-colour multiplexed western blot against a complex cell lysate background, and have significantly improved previously reported non-specific binding of the Qdots to cellular proteins.

Purification of plasmid DNA for use in human gene therapy

Two key issues defined the focus of this research in manufacturing plasmid DNA for use In human gene therapy. First, the processing of E.coli bacterial cells to effect the separation of therapeutic plasmid DNA from cellular debris and adventitious material. Second, the affinity purification of the plasmid DNA in a Simple one-stage process. The need arises when considering the concerns that have been recently voiced by the FDA concerning the scalability and reproducibility of the current manufacturing processes in meeting the quality criteria of purity, potency, efficacy, and safety for a recombinant drug substance for use in humans. To develop a preliminary purification procedure, an EFD cross-flow micro-filtration module was assessed for its ability to effect the 20-fold concentration, 6-time diafiltration, and final clarification of the plasmid DNA from the subsequent cell lysate that is derived from a 1 liter E.coli bacterial cell culture. Historically, the employment of cross-flow filtration modules within procedures for harvesting cells from bacterial cultures have failed to reach the required standards dictated by existing continuous centrifuge technologies, frequently resulting in the rapid blinding of the membrane with bacterial cells that substantially reduces the permeate flux. By challenging the EFD module, containing six helical wound tubular membranes promoting centrifugal instabilities known as Dean vortices, with distilled water between the Dean number's of 187Dn and 818Dn,and the transmembrane pressures (TMP) of 0 to 5 psi. The data demonstrated that the fluid dynamics significantly influenced the permeation rate, displaying a maximum at 227Dn (312 Imh) and minimum at 818Dn (130 Imh) for a transmembrane pressure of 1 psi. Numerical studies indicated that the initial increase and subsequent decrease resulted from a competition between the centrifugal and viscous forces that create the Dean vortices. At Dean numbers between 187Dn and 227Dn , the forces combine constructively to increase the apparent strength and influence of the Dean vortices. However, as the Dean number in increases above 227 On the centrifugal force dominates the viscous forces, compressing the Dean vortices into the membrane walls and reducing their influence on the radial transmembrane pressure i.e. the permeate flux reduced. When investigating the action of the Dean vortices in controlling tile fouling rate of E.coli bacterial cells, it was demonstrated that the optimum cross-flow rate at which to effect the concentration of a bacterial cell culture was 579Dn and 3 psi TMP, processing in excess of 400 Imh for 20 minutes (i.e., concentrating a 1L culture to 50 ml in 10 minutes at an average of 450 Imh). The data demonstrated that there was a conflict between the Dean number at which the shear rate could control the cell fouling, and the Dean number at which tile optimum flux enhancement was found. Hence, the internal geometry of the EFD module was shown to sub-optimal for this application. At 579Dn and 3 psi TMP, the 6-fold diafiltration was shown to occupy 3.6 minutes of process time, processing at an average flux of 400 Imh. Again, at 579Dn and 3 psi TMP the clarification of the plasmid from tile resulting freeze-thaw cell lysate was achieved at 120 Iml1, passing 83% (2,5 mg) of the plasmid DNA (6,3 ng μ-1 10.8 mg of genomic DNA (∼23,00 Obp, 36 ng μ-1 ), and 7.2 mg of cellular proteins (5-100 kDa, 21.4 ngμ-1 ) into the post-EFD process stream. Hence the EFD module was shown to be effective, achieving the desired objectives in approximately 25 minutes. On the basis of its ability to intercalate into low molecular weight dsDNA present in dilute cell lysates, and be electrophoresed through agarose, the fluorophore PicoGreen was selected for the development of a suitable dsDNA assay. It was assesseel for its accuracy, and reliability, In determining the concentration and identity of DNA present in samples that were eleclrophoresed through agarose gels. The signal emitted by intercalated PicoGreen was shown to be constant and linear, and that the mobility of the PicaGreen-DNA complex was not affected by the intercalation. Concerning the secondary purification procedure, various anion-exchange membranes were assessed for their ability to capture plasmid DNA from the post-EFD process stream. For a commercially available Sartorius Sartobind Q15 membrane, the reduction in the equilibriumbinding capacity for  ctDNA in buffer of increasing ionic demonstrated that DNA was being.adsorbed by electrostatic  interactions only. However, the problems associated with fluid distribution across the membrane demonstrated that the membrane housing was the predominant cause of the .erratic breakthrough curves. Consequently, this would need to be rectified before such a membrane could be integrated into the current system, or indeed be scaled beyond laboratory scale. However, when challenged with the process material, the data showed that considerable quantities of protein (1150 μg) were adsorbed preferentially to the plasmid DNA (44 μg). This was also shown for derived Pall Gelman UltraBind US450 membranes that had been functionalised by varying molecular weight poly-L~lysine and polyethyleneimine ligands. Hence the anion-exchange membranes were shown to be ineffective in capturing plasmid DNA from the process stream. Finally, work was performed to integrate a sequence-specific DNA·binding protein into a single-stage DNA chromatography, isolating plasmid DNA from E.coli cells whilst minimising the contamination from genomic DNA and cellular protein. Preliminary work demonstrated that the fusion protein was capable of isolating pUC19 DNA into which the recognition sequence for the fusion-protein had been inserted (pTS DNA) when in the presence of the conditioned process material. Althougth the pTS recognition sequence differs from native pUC19 sequences by only 2 bp, the fusion protein was shown to act as a highly selective affinity ligand for pTS DNA alone. Subsequently, the scale of the process was scaled 25-fold and positioned directly following the EFD system. In conclusion, the integration of the EFD micro-filtration system and zinc-finger affinity purification technique resulted in the capture of approximately 1 mg of plasmid DNA was purified from 1L of E.coli  culture in a simple two stage process, resulting in the complete removal of genomic DNA and 96.7% of cellular protein in less than 1 hour of process time.

Simple design of nonuniform fiber Bragg grating with sharp reflection

In this paper we consider the possibility of using intermediate solutions, in which ideal apodisation profile for a dispersion-free, sharp-reflection profile fibre Bragg grating approximated in different degrees. The ideal apodisation profile for a flat dispersion, 50 GHz bandwidth grating was obtained using the layer-peeling algorithm. To verify the modelled results a version of the 5-section grating has been manufactured with excellent agreement between the model and the experimental results. The performance penalty due to multiple reflections from the FBGs in different situations was studied. The results showed that in the approximated gratings some post-compensation must be included to account for the local deviations from zero dispersion. © 2003 IEEE.

A simple method for the fabrication of intrinsically apodized chirped fibre Bragg gratings

A new and simple fabrication technique is reported for the UV inscription of intrinsically apodized chirped fibre gratings at an arbitrary Bragg wavelength employing a single chirped phase-mask in a scanning Talbot interferometer set-up. Chirped gratings have been successfully produced over a large wavelength range and with bandwidths up to 5 nm. These gratings exhibit the time-delay response of a small ripple effect. In the present paper a comparison with previously reported fabrication methods is given, showing the advantages and disadvantages of the different methods.

Il était une fois le passé simple...

Depuis plus d’un siècle, les linguistes débattent de la disparition du passé simple. Le PS est un paradigme moribond pour les uns, une forme d’emploi toujours restreint ou encore en voie de spécialisation pour d’autres. La discussion sur le devenir du PS ne prend cependant son sens que sous un éclairage diachronique. Le présent article examine donc les emplois du PS au travers de l’histoire de la langue française. Si le PS a perdu une fonction depuis l’ancien français et est sorti de l’oral spontané, il n’en jouit pas moins d’une place privilégiée dans certains contextes (narration) et cotextes (3èmes personnes). Le tiroir semble même avoir récemment investi de nouvelles formes d’expression, comme le storytelling en mercatique. L'étude conclut en suggérant des pistes de recherche pour évaluer la position réelle du PS en français contemporain.