Discriminating depth in stereo surfaces: dipper effect found, and lost

Measurement of detection and discrimination thresholds yields information about visual signal processing. For luminance contrast, we are 2 - 3 times more sensitive to a small increase in the contrast of a weak 'pedestal' grating, than when the pedestal is absent. This is the 'dipper effect' - a reliable improvement whose interpretation remains controversial. Analogies between luminance and depth (disparity) processing have attracted interest in the existence of a 'disparity dipper' - are thresholds for disparity, or disparity modulation (corrugated surfaces), facilitated by the presence of a weak pedestal? Lunn and Morgan (1997 Journal of the Optical Society of America A 14 360 - 371) found no dipper for disparity-modulated gratings, but technical limitations (8-bit greyscale) might have prevented the necessary measurement of very small disparity thresholds. We used a true 14-bit greyscale to render small disparities accurately, and measured 2AFC discrimination thresholds for disparity modulation (0.6 cycle deg-1) of a random texture at various pedestal levels. Which interval contained greater modulation of depth? In the first experiment, a clear dipper was found. Thresholds were about 2X1 lower with weak pedestals than without. But here the phase of modulation (0° or 180°) was randomised from trial to trial. In a noisy signal-detection framework, this creates uncertainty that is reduced by the pedestal, thus improving performance. When the uncertainty was eliminated by keeping phase constant within sessions, the dipper effect disappeared, confirming Lunn and Morgan's result. The absence of a dipper, coupled with shallow psychometric slopes, suggests that the visual response to small disparities is essentially linear, with no threshold-like nonlinearity.

Paradox lost? Exploring the role of alpha oscillations during externally vs. internally directed attention and the implications for idling and inhibition hypotheses

Although slow waves of the electroencephalogram (EEG) have been associated with attentional processes, the functional significance of the alpha component in the EEG (8.1–12 Hz) remains uncertain. Conventionally, synchronisation in the alpha frequency range is taken to be a marker of cognitive inactivity, i.e. ‘cortical idling’. However, it has been suggested that alpha may index the active inhibition of sensory information during internally directed attentional tasks such as mental imagery. More recently, this idea has been amended to encompass the notion of alpha synchronisation as a means of inhibition of non-task relevant cortical areas irrespective of the direction of attention. Here we test the adequacy of the one idling and two inhibition hypotheses about alpha. In two experiments we investigated the relation between alpha and internally vs. externally directed attention using mental imagery vs. sensory-intake paradigms. Results from both experiments showed a clear relationship between alpha and both attentional factors and increased task demands. At various scalp sites alpha amplitudes were greater during internally directed attention and during increased load, results incompatible with alpha reflecting cortical idling and more in keeping with suggestions of active inhibition necessary for internally driven mental operations.

Hijacked then lost in translation:the plight of the recombinant host cell in membrane protein structural biology projects

Membrane protein structural biology is critically dependent upon the supply of high-quality protein. Over the last few years, the value of crystallising biochemically characterised, recombinant targets that incorporate stabilising mutations has been established. Nonetheless, obtaining sufficient yields of many recombinant membrane proteins is still a major challenge. Solutions are now emerging based on an improved understanding of recombinant host cells; as a 'cell factory' each cell is tasked with managing limited resources to simultaneously balance its own growth demands with those imposed by an expression plasmid. This review examines emerging insights into the role of translation and protein folding in defining high-yielding recombinant membrane protein production in a range of host cells.

Paradigms lost:integrating history and organization studies

This article reviews recent attempts at mapping research paradigms in Management and Organizational History and argues that the old distinctions between supplementarist, integrationist, and reorientationist approaches have been superseded by attempts at integrating historical research in organization studies. A typology of these integrationist approaches differentiates between pluralist and unitary integration, as well as between models based on either historical theory or organization theory. Each has distinct weaknesses and strengths, but essentially all limit their integration of historical research paradigms to only a few. As a result, there is a danger that history might become reduced to a methodology, an empirical endeavor, narrative representations, or indeed be considered the subject of research rather than a research approach in its own right. I argue that all of these present an impoverished picture of the rich research traditions available in the discipline of history, which has unique insights and approaches to offer to the study of organizations.