Transcriptome analysis of a respiratory Saccharomyces cerevisiae strain suggests the expression of its phenotype is glucose insensitive and predominantly controlled by Hap4, Cat8 and Mig1

BACKGROUND: We previously described the first respiratory Saccharomyces cerevisiae strain, KOY.TM6*P, by integrating the gene encoding a chimeric hexose transporter, Tm6*, into the genome of an hxt null yeast. Subsequently we transferred this respiratory phenotype in the presence of up to 50 g/L glucose to a yeast strain, V5 hxt1-7Delta, in which only HXT1-7 had been deleted. In this study, we compared the transcriptome of the resultant strain, V5.TM6*P, with that of its wild-type parent, V5, at different glucose concentrations. RESULTS: cDNA array analyses revealed that alterations in gene expression that occur when transitioning from a respiro-fermentative (V5) to a respiratory (V5.TM6*P) strain, are very similar to those in cells undergoing a diauxic shift. We also undertook an analysis of transcription factor binding sites in our dataset by examining previously-published biological data for Hap4 (in complex with Hap2, 3, 5), Cat8 and Mig1, and used this in combination with verified binding consensus sequences to identify genes likely to be regulated by one or more of these. Of the induced genes in our dataset, 77% had binding sites for the Hap complex, with 72% having at least two. In addition, 13% were found to have a binding site for Cat8 and 21% had a binding site for Mig1. Unexpectedly, both the up- and down-regulation of many of the genes in our dataset had a clear glucose dependence in the parent V5 strain that was not present in V5.TM6*P. This indicates that the relief of glucose repression is already operable at much higher glucose concentrations than is widely accepted and suggests that glucose sensing might occur inside the cell. CONCLUSION: Our dataset gives a remarkably complete view of the involvement of genes in the TCA cycle, glyoxylate cycle and respiratory chain in the expression of the phenotype of V5.TM6*P. Furthermore, 88% of the transcriptional response of the induced genes in our dataset can be related to the potential activities of just three proteins: Hap4, Cat8 and Mig1. Overall, our data support genetic remodelling in V5.TM6*P consistent with a respiratory metabolism which is insensitive to external glucose concentrations.

Bend and index insensitive long period grating in progressive three layered optical fibre

A long period fibre grating written in a progressive three-layered optical fibre is shown to exhibit an attenuation band that has a very low bending sensitivity (5.1×10 –2 nm m) compared to normal step-index fibre, and is also insensitive to changes in the refractive index of the surrounding medium. Applications to sensing and telecommunications are discussed.

Temperature-insensitive long period grating sensors in photonic crystal fibre

A long period grating has been fabricated in endlessly single-mode photonic crystal fibre using a spatially-periodic electric arc discharge. The sensing characteristics of the grating have been studied and it was found to possess an insensitivity to temperature, a bend sensitivity of 3.7 nm · m and a strain sensitivity of -2.0 pm/µe.

Thermal insensitive optical liquid level sensor based on excessively tilted fibre Bragg grating

We demonstrate a liquid level sensor based on the surrounding medium refractive index (SRI) sensing using of an excessively tilted fibre Bragg grating (ETFBG). The sensor has low thermal cross sensitivity and high SRI responsivity.

Two-axis temperature-insensitive accelerometer based on multicore fiber Bragg gratings

We report a compact two-dimensional accelerometer based upon a simple fiber cantilever constructed from a short length of multicore optical fiber. Two-axis measurement is demonstrated up to 3 kHz. Differential measurement between fiber Bragg gratings written in the multicore fiber provides temperature- insensitive measurements.

Molecular basis for reduced estrone sulfate transport and altered modulator sensitivity of transmembrane helix (TM) 6 and TM17 mutants of multidrug resistance protein 1 (ABCC1)

Multidrug resistance protein 1 (MRP1) confers drug resistance and also mediates cellular efflux of many organic anions. MRP1 also transports glutathione (GSH); furthermore, this tripeptide stimulates transport of several substrates, including estrone 3-sulfate. We have previously shown that mutations of Lys(332) in transmembrane helix (TM) 6 and Trp(1246) in TM17 cause different substrate-selective losses in MRP1 transport activity. Here we have extended our characterization of mutants K332L and W1246C to further define the different roles these two residues play in determining the substrate and inhibitor specificity of MRP1. Thus, we have shown that TM17-Trp(1246) is crucial for conferring drug resistance and for binding and transport of methotrexate, estradiol glucuronide, and estrone 3-sulfate, as well as for binding of the tricyclic isoxazole inhibitor N-[3-(9-chloro-3-methyl-4-oxo-4H-isoxazolo-[4,3-c]quinolin-5-yl)-cyclohexylmethyl]-benzamide (LY465803). In contrast, TM6-Lys(332) is important for enabling GSH and GSH-containing compounds to serve as substrates (e.g., leukotriene C(4)) or modulators (e.g., S-decyl-GSH, GSH disulfide) of MRP1 and, further, for enabling GSH (or S-methyl-GSH) to enhance the transport of estrone 3-sulfate and increase the inhibitory potency of LY465803. On the other hand, both mutants are as sensitive as wild-type MRP1 to the non-GSH-containing inhibitors (E)-3-[[[3-[2-(7-chloro-2-quinolinyl)ethenyl]phenyl][[3-(dimethylamino)-3-oxopropyl]thio]methyl]thio]-propanoic acid (MK571), 1-[2-hydroxy-3-propyl-4-[4-(1H-tetrazol-5-yl)butoxy]phenyl]-ethanone (LY171883), and highly potent 6-[4'-carboxyphenylthio]-5[S]-hydroxy-7[E], 11[Z]14[Z]-eicosatetrenoic acid (BAY u9773). Finally, the differing abilities of the cysteinyl leukotriene derivatives leukotriene C(4), D(4), and F(4) to inhibit estradiol glucuronide transport by wild-type and K332L mutant MRP1 provide further evidence that TM6-Lys(332) is involved in the recognition of the gamma-Glu portion of substrates and modulators containing GSH or GSH-like moieties.

Humidity insensitive TOPAS polymer fiber Bragg grating sensor

We report the first experimental demonstration of a humidity insensitive polymer optical fiber Bragg grating (FBG), as well as the first FBG recorded in a TOPAS polymer optical fiber in the important low loss 850nm spectral region. For the demonstration we have fabricated FBGs with resonance wavelength around 850 nm and 1550 nm in single-mode microstructured polymer optical fibers made of TOPAS and the conventional poly (methyl methacrylate) (PMMA). Characterization of the FBGs shows that the TOPAS FBG is more than 50 times less sensitive to humidity than the conventional PMMA FBG in both wavelength regimes. This makes the TOPAS FBG very appealing for sensing applications as it appears to solve the humidity sensitivity problem suffered by the PMMA FBG. © 2011 Optical Society of America.

Thermal insensitive optical liquid level sensor based on excessively tilted fibre Bragg grating

We demonstrate a liquid level sensor based on the surrounding medium refractive index (SRI) sensing using of an excessively tilted fibre Bragg grating (ETFBG). The sensor has low thermal cross sensitivity and high SRI responsivity.

Bend and index insensitive long period grating in progressive three layered optical fibre

A long period fibre grating written in progressive three layered optical fibre was examined. The bending sensitivity of the optical fibre was measured. It was found that the fibre shows an attenuation band that has a very low bending sensitivity compared to normal step-index fibres.

Temperature insensitive long-period grating sensors in photonic crystal fibre

Presented are long-period gratings (LPGs) fabricated in pure silica photonic crystal fibre (PCF) using an electric arc. Two different varieties of PCF have been investigated, an endlessly single mode PCF and a large-mode area PCF. The LPGs have been characterised for their sensitivity to a variety of external measurands. The LPGs in both fibres have been found to have negligible temperature sensitivity whilst exhibiting good sensitivity to bending and strain.

Humidity insensitive TOPAS polymer fiber Bragg grating sensor

We report the first experimental demonstration of a humidity insensitive polymer optical fiber Bragg grating (FBG), as well as the first FBG recorded in a TOPAS polymer optical fiber in the important low loss 850nm spectral region. For the demonstration we have fabricated FBGs with resonance wavelength around 850 nm and 1550 nm in single-mode microstructured polymer optical fibers made of TOPAS and the conventional poly (methyl methacrylate) (PMMA). Characterization of the FBGs shows that the TOPAS FBG is more than 50 times less sensitive to humidity than the conventional PMMA FBG in both wavelength regimes. This makes the TOPAS FBG very appealing for sensing applications as it appears to solve the humidity sensitivity problem suffered by the PMMA FBG. © 2011 Optical Society of America.

Temperature-insensitive long period grating sensors in photonic crystal fibre

A long period grating has been fabricated in endlessly single-mode photonic crystal fibre using a spatially-periodic electric arc discharge. The sensing characteristics of the grating have been studied and it was found to possess an insensitivity to temperature, a bend sensitivity of 3.7 nm · m and a strain sensitivity of -2.0 pm/µe.

Polarization insensitive in-fiber mode-locker based on carbon nanotube with N-methyl-2-pryrrolidone solvent filled fiber microchamber

We report an in-fiber laser mode locker based on carbon nanotube with n-methyl-2-pryrrolidone solvent filled in-fiber microchamber. Symmetrically femtosecond laser fabricated in-fiber microchamber with randomly oriented nanotubes assures polarization insensitive oscillation of laser mode locking. The proposed and demonstrated passively mode locked fiber laser shows higher energy soliton output. The laser has an output power of ∼29 mW (corresponding to 11 nJ energy). It shows stable soliton output with a repetition rate of ∼2.3 MHz and pulse width of ∼3.37 ps. © 2012 American Institute of Physics.

4 Tb/s transmission reach enhancement using 10 × 400 Gb/s super-channels and polarization insensitive dual band optical phase conjugation

In this paper, we experimentally demonstrate the benefit of polarization insensitive dual-band optical phase conjugation for up to ten 400 Gb/s optical super-channels using a Raman amplified transmission link with a realistic span length of 75 km. We demonstrate that the resultant increase in transmission distance may be predicted analytically if the detrimental impacts of power asymmetry and polarization mode dispersion are taken into account.

4 Tb/s transmission reach enhancement using 10 × 400 Gb/s super-channels and polarization insensitive dual band optical phase conjugation

In this paper, we experimentally demonstrate the benefit of polarization insensitive dual-band optical phase conjugation for up to ten 400 Gb/s optical super-channels using a Raman amplified transmission link with a realistic span length of 75 km. We demonstrate that the resultant increase in transmission distance may be predicted analytically if the detrimental impacts of power asymmetry and polarization mode dispersion are taken into account.