Retromer controls epithelial cell polarity by trafficking the apical determinant crumbs

The evolutionarily conserved apical determinant Crumbs (Crb) is essential for maintaining apicobasal polarity and integrity of many epithelial tissues [1]. Crb levels are crucial for cell polarity and homeostasis, yet strikingly little is known about its trafficking or the mechanism of its apical localization. Using a newly established, liposome-based system described here, we determined Crb to be an interaction partner and cargo of the retromer complex. Retromer is essential for the retrograde transport of numerous transmembrane proteins from endosomes to the trans-Golgi network (TGN) and is conserved between plants, fungi, and animals [2]. We show that loss of retromer function results in a substantial reduction of Crb in Drosophila larvae, wing discs, and the follicle epithelium. Moreover, loss of retromer phenocopies loss of crb by preventing apical localization of key polarity molecules, such as atypical protein kinase C (aPKC) and Par6 in the follicular epithelium, an effect that can be rescued by overexpression of Crb. Additionally, loss of retromer results in multilayering of the follicular epithelium, indicating that epithelial integrity is severely compromised. Our data reveal a mechanism for Crb trafficking by retromer that is vital for maintaining Crb levels and localization. We also show a novel function for retromer in maintaining epithelial cell polarity.

Determinant of broadband internet adoption and continuance in Singapore

In spite of increasing significance of Broadband Internet, there are not many research papers explicitly addressing issues pertaining to its deployment and continuance. Previous research on Broadband has mainly focused on the supply side aspect at the national level, ignoring the importance of the demand side which may involve looking more deeply into the factors impacting organizational and individual uptake. In an attempt to fill this gap, the current study empirically verifies the IS continuance model to examine factors influencing Broadband Internet post-adoption behavior of some 1,500 organizations in Singapore. Strong support for the model has been manifested by our results, providing insight into influential factors. Results of the study suggest that that perceived usefulness is the strongest predictor of users' continuance intention, followed by satisfaction with Broadband Internet usage as a significant but weaker predictor.