Shaping acoustic fields as a toolset for microfluidic manipulations in diagnostic technologies

Ultrasonics offers the possibility of developing sophisticated fluid manipulation tools in lab-on-a-chip technologies. Here we demonstrate the ability to shape ultrasonic fields by using phononic lattices, patterned on a disposable chip, to carry out the complex sequence of fluidic manipulations required to detect the rodent malaria parasite Plasmodium berghei in blood. To illustrate the different tools that are available to us, we used acoustic fields to produce the required rotational vortices that mechanically lyse both the red blood cells and the parasitic cells present in a drop of blood. This procedure was followed by the amplification of parasitic genomic sequences using different acoustic fields and frequencies to heat the sample and perform a real-time PCR amplification. The system does not require the use of lytic reagents nor enrichment steps, making it suitable for further integration into lab-on-a-chip point-of-care devices. This acoustic sample preparation and PCR enables us to detect ca. 30 parasites in a microliter-sized blood sample, which is the same order of magnitude in sensitivity as lab-based PCR tests. Unlike other lab-on-a-chip methods, where the sample moves through channels, here we use our ability to shape the acoustic fields in a frequency-dependent manner to provide different analytical functions. The methods also provide a clear route toward the integration of PCR to detect pathogens in a single handheld system.

Determining planar multiple sound-soft obstacles from scattered acoustic fields

An inverse problem is considered where the structure of multiple sound-soft planar obstacles is to be determined given the direction of the incoming acoustic field and knowledge of the corresponding total field on a curve located outside the obstacles. A local uniqueness result is given for this inverse problem suggesting that the reconstruction can be achieved by a single incident wave. A numerical procedure based on the concept of the topological derivative of an associated cost functional is used to produce images of the obstacles. No a priori assumption about the number of obstacles present is needed. Numerical results are included showing that accurate reconstructions can be obtained and that the proposed method is capable of finding both the shapes and the number of obstacles with one or a few incident waves.

Ultrasonic field and temperature sensor based on short in-fibre Bragg gratings

The authors demonstrate that in-fibre Bragg gratings may be successfully used to measure megahertz acoustic fields if the grating length is sufficiently short and the optical fibre is appropriately desensitised. A noise-limited pressure resolution of 4.5 × 10 –3 atm vHz was found. The capability to simultaneously act as a temperature sensor is also demonstrated.

Combined ultrasound and temperature sensor using a fibre Bragg grating

We demonstrate the feasibility of using in-fibre Bragg gratings to measure MHz acoustic fields and temperature simultaneously. We achieved a noise-limited pressure resolution of ˜4.5×10-4 Atm/vHz and a temperature resolution of 0.2°C.

Ultrasonic field and temperature sensor based on short in-fibre Bragg gratings

The authors demonstrate that in-fibre Bragg gratings may be successfully used to measure megahertz acoustic fields if the grating length is sufficiently short and the optical fibre is appropriately desensitised. A noise-limited pressure resolution of 4.5 × 10 –3 atm vHz was found. The capability to simultaneously act as a temperature sensor is also demonstrated.

Ultrasonic hydrophone based on short in-fiber Bragg gratings

We investigate the feasibility of using in-fiber Bragg gratings for measuring acoustic fields in the megahertz range. We found that the acoustic coupling from the ultrasonic field to the grating leads to the formation of standing waves in the fiber. Because of these standing waves, the system response is complex and, as we show, the grating does not act as an effective probe. However, significant improvement in its performance can be gained by use of short gratings coupled with an appropriate desensitization of the fiber. A noise-limited pressure resolution of ˜4.5 × 10-3 atm/vHz was found.