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RV POSEIDON cruise POS264 was carried out by the Institut für Meereskunde of the University of Hamburg and staff from the Niels Bohr Instituttet for Astronomi, Fysik og Geofysik of the University of Copenhagen also participated. The cruise had several objectives: - to educate undergraduate students in the handling of oceanographic instrumentation and in the collection and analysis of field data, - to map the cold overflow through the Faroe-Bank Channel from the Norwegian Sea into the Icelandic Basin and to study its short-time variability and - to quantify the contributions of the water masses which are involved in the mixing of the overflow plume with its ambient water. The planning and preparation of the cruise involved the participating students and was carried out during seminars, both at the Universities of Hamburg and Copenhagen. Following a review of the recent literature and an analysis of historical data the observational programme was designed. Hydrographic and current profiling stations were occupied along several sections crossing the overflow. The experiment was financed by the University of Hamburg. Temperature, salinity and dissolved oxygen data from CTD stations are presented. The temperature and salinity data were quality controlled and calibrated. Oxygen data are not calibrated as no oxygen samples were taken additionally during the cruise.