Gene sequences of the lichen Cetraria aculeata

Lichens are symbioses between fungi (mycobionts) and photoautotrophic green algae or cyanobacteria (photobionts). Many lichens occupy large distributional ranges covering several climatic zones. So far, little is known about the large-scale phylogeography of lichen photobionts and their role in shaping the distributional ranges of lichens. We studied south polar, temperate and north polar populations of the widely distributed fruticose lichen Cetraria aculeata. Based on the DNA sequences from three loci for each symbiont, we compared the genetic structure of mycobionts and photobionts. Phylogenetic reconstructions and Bayesian clustering methods divided the mycobiont and photobiont data sets into three groups. An AMOVA shows that the genetic variance of the photobiont is best explained by differentiation between temperate and polar regions and that of the mycobiont by an interaction of climatic and geographical factors. By partialling out the relative contribution of climate, geography and codispersal, we found that the most relevant factors shaping the genetic structure of the photobiont are climate and a history of codispersal. Mycobionts in the temperate region are consistently associated with a specific photobiont lineage. We therefore conclude that a photobiont switch in the past enabled C. aculeata to colonize temperate as well as polar habitats. Rare photobiont switches may increase the geographical range and ecological niche of lichen mycobionts by associating them with locally adapted photobionts in climatically different regions and, together with isolation by distance, may lead to genetic isolation between populations and thus drive the evolution of lichens.

Zooplankton abundance for two shelf stations off Galicia (NW Spain) from 1994 to 2006

Zooplankton was sampled by project RADIALES at Vigo (E3VI) and A Coruña (E2CO) between 1994 and 2006. Samples were collected using 50-cm diameter Juday-Bogorov (A Coruña) or 40-cm diameter bongo plankton nets (Vigo) equipped with 200-µm mesh size. Tows were double oblique from surface to near bottom (90 and 70 m in Vigo and A Coruña, respectively). All samples were collected between 10:00 and 14:00 o'clock (local time). Samples were preserved in 2-4% sodium borate-buffered formaldehyde. For the purpose of this study, the original coastal time series were categorized in copepods representative of crustacean zooplankton) and gelatinous plankton (medusae and tunicates). Medusae included Hydrozoans and Scyphozoa, and tunicates included salps, pyrosomes, doliolids, and appendicularia. Plankton identification and counts were performed by Ana Miranda and M. Teresa Álvarez-Ossorio for samples from Vigo and A Coruña, respectively. Different trends were found for gelatinous plankton in the two coastal sites, characterized by increases in either medusae or tunicates. Multiyear periods of relative dominance of gelatinous vs. copepod plankton were evident. In general, copepod periods were observed in positive phases of the main modes of regional climatic variability. Conversely, gelatinous periods occurred during negative climatic phases. However, the low correlations between gelatinous plankton and either climatic, oceanographic, or fishery variables suggest that local factors play a major role in their proliferations.