The effect of elevated CO2 and increased temperature on in vitro fertilization success and initial embryonic development of single male:female crosses of broad-cast spawning corals at mid- and high-latitude locations

The impact of global climate change on coral reefs is expected to be most profound at the sea surface, where fertilization and embryonic development of broadcast-spawning corals takes place. We examined the effect of increased temperature and elevated CO2 levels on the in vitro fertilization success and initial embryonic development of broadcast-spawning corals using a single male:female cross of three different species from mid- and high-latitude locations: Lyudao, Taiwan (22° N) and Kochi, Japan (32° N). Eggs were fertilized under ambient conditions (27 °C and 500 µatm CO2) and under conditions predicted for 2100 (IPCC worst case scenario, 31 °C and 1000 µatm CO2). Fertilization success, abnormal development and early developmental success were determined for each sample. Increased temperature had a more profound influence than elevated CO2. In most cases, near-future warming caused a significant drop in early developmental success as a result of decreased fertilization success and/or increased abnormal development. The embryonic development of the male:female cross of A. hyacinthus from the high-latitude location was more sensitive to the increased temperature (+4 °C) than the male:female cross of A. hyacinthus from the mid-latitude location. The response to the elevated CO2 level was small and highly variable, ranging from positive to negative responses. These results suggest that global warming is a more significant and universal stressor than ocean acidification on the early embryonic development of corals from mid- and high-latitude locations.

Geochemistry of volcanivlastic sediments of ODP Sites 134-832 and 134-833

Volcaniclastic sediments of North Aoba Basin (Vanuatu) recovered during Ocean Drilling Program (ODP) Leg 134 show a mineralogical and chemical overprint of low grade hydrothermal alteration superimposed on the primary magmatic source compositions. The purpose of this study was to identify authigenic mineral phases incorporated in the volcaniclastic sediments, to distinguish authigenic chemical and mineralogical signals from the original volcaniclastic mineralogical and chemical compositions, and to determine the mechanism of authigenic minerals formation. Mineralogical, micro-chemical and bulk chemical analyses were utilized to identify and characterize authigenic phases and determine the original unaltered ash compositions. 117 volcaniclastic sediment samples from North Aoba Basin Sites 832 and 833 were analyzed. Primary volcaniclastic materials accumulated in North Aoba Basin can be divided into three types. The older basin-filling sequences show three different magmatic trends: high K, calc-alkaline, and low K series. The most recent accumulations are rhyodacitic composition and can be attributed to Santa Maria or Aoba volcanic emissions. Original depositional porosity of volcaniclastic sediments is an important factor in influencing distribution of authigenic phases. Finer-grained units are less altered and retain a bulk mineralogical and chemical composition close to the original pyroclastic rock composition. Coarser grained units (microbreccia and sandstones) are the major hosts of authigenic minerals. At both sites, authigenic minerals (including zeolites, clay minerals, Mg-carbonates, and quartz) exhibit complex zonation with depth that crosses original ash depositional boundaries and stratigraphic limits. The zeolite minerals phillipsite and analcime are ubiquitous throughout the altered intervals. At Site 832, the first zeolite minerals (phillipsite) occur in Pleistocene deposits as shallow as 146 meters below seafloor (mbsf). At Site 833 the first zeolite minerals (analcime) occur in Pleistocene deposits as shallow as 224 mbsf. The assemblage phillipsite + analcime + chabazite appears at 635 mbsf (Site 832) and at 376 mbsf (Site 833). Phillipsite + analcime + chabazite + thomsonite + heulandite are observed between 443 and 732 mbsf at Site 833. Thomsonite is no longer observed below 732 mbsf at Site 833. Heulandite is present to the base of the sections cored. The zeolite assemblages are associated with authigenic clay minerals (nontronite and saponite), calcite, and quartz. Chlorite is noticeable at Site 832 as deep as 851 mbsf. Zeolite zones are present but are less well defined at Site 832. Dolomite and rare magnesite are present below 940 m at Site 832. The coarse-grained authigenic mineral host intervals exhibit geochemical signatures that can be attributed to low grade hydrothermal alteration. The altered intervals show evidence of K2O, CaO, and rare earth elements mobilization. When compared to fine-grained, unaltered units, and to Santa Maria Island volcanics rocks, the altered zones are relatively depleted in rare earth elements, with light rare earth elements-heavy rare earth elements fractionation. Drilling at Site 833 penetrated a sill complex below 840 m. No sill was encountered at Site 832. Complex zonation of zeolite facies, authigenic smectites, carbonates and quartz, and associated geochemical signatures are present at both sites. The mineralogical and chemical alteration overprint is most pronounced in the deeper sections at Site 832. Based on mineralogical and chemical evidence at two locations less than 50 km apart, there is vertical and lateral variation in alteration of the volcaniclastic sediments of North Aoba Basin. The alteration observed may be activated by sill intrusion and associated expulsion of heated fluids into intervals of greater porosity. Such spatial variation in alteration could be attributed to the evolution of the basin axis associated with subduction processes along the New Hebrides Trench.

Geochemistry at DSDP Hole 57-439

Dating of a hornblende concentrate by the 40Ar/39Ar method gives an age of 23.4±5.5 m.y. for a dacite boulder from conglomerate in Deep Sea Drilling Project Hole 439. The conglomerate clasts range up to 1 meter in diameter and are nearly monolithologic, suggesting that a nearby former volcano erupted the dacite. The dacite is only 90 km landward from the Japan Trench, whereas modern trench-related volcanoes lie at least 120 km from their trenches. The dacite locality is on strike with and is probably an extension of a magmatic arc on the island of Hokkaido that crosses the Kuril arc at an angle of 65° and which was active 16 to 36 m.y. ago. The part of the former arc landward from the Kuril arc argues against an origin from a leaking subduction zone or from subduction of an active spreading ridge. The part seaward both from the Kuril and Japan arcs weakens an explanation based on migration of a trench-trenchtrench triple junction. The magmatic rocks probably formed along a middle-Tertiary plate boundary that had stepped seaward from a more-landward Cretaceous position. Later, the boundary stepped farther seaward at the Kuril arc and landward again at the Japan arc. If so, the present Japan subduction zone must have consumed most of the strata that had accumulated between it and the earlier trench.

Middle Eocene to Pleistocene calcareous nannofossils of ODP Leg 113 holes

Long sequences of Upper Cretaceous through Quaternary sediments rich in calcareous and siliceous microfossils were recovered at Ocean Drilling Program Sites 689 and 690 on Maud Rise off East Antarctica. These sites have become the southernmost anchor in the Atlantic Basin for bio-, magneto-, chemostratigraphic, and paleobiogeographic studies. ODP Sites 692 and 693 on the Weddell Sea margin of East Antarctica and Site 696 on the South Orkney microcontinent of West Antarctica yielded calcareous nannofossils within some stratigraphic intervals. Sites 691, 692, 694, 695, and 697 did not recover Cenozoic calcareous nannofossils. Calcareous nannofossil biostratigraphy suggests a major hiatus across the Paleogene/Neogene boundary at Sites 689 and 690, and two additional hiatuses in the middle Eocene-lower Oligocene section at Site 690. Correlation with magnetostratigraphy reveals: the last occurrence (LO) of Reticulofenestra umbilica at Maud Rise is over 1 m.y. younger than that at the middle-latitude sites; the LO of Isthmolithus recurvus is synchronous in the middle-latitude and high-latitude areas (about 34.8 Ma); Reticulofenestra oamaruensis ranges from 38.0 to 36.0 Ma at Maud Rise; Reticulofenestra reticulata has a shorter range at Maud Rise (42.1 to 38.9 Ma) than at the middle-latitude DSDP Site 516; the range of Chiasmolithus oamaruensis is diachronous over different latitudes; and the LO of Chiasmolithus solitus is a good datum at 41.3 Ma from 30°S to 65°S in the South Atlantic Ocean. Comparison of calcareous nannofossil abundances in a latitudinal transect shows: Reticulofenestra bisecta is a temperate-water species and its LO, which crosses below that of Chiasmolithus altus at Maud Rise, is not applicable for the Paleogene/Neogene boundary in high southern latitude areas; Clausicoccus fenestratus is rare or absent at Maud Rise and can not be used as a marker; Coccolithus formosus is a warm-water species which disappeared earlier toward higher latitudes. Calcareous nannofossil assemblages indicate that by at least the middle Eocene, surface water temperatures became considerably lower in the high southern latitudes than in the middle-latitude areas and that there have been more extreme cold events in the high latitudes during the Neogene. Bicolumnus ovatus n. gen., n. sp. is proposed in this paper.

Swath sonar bathymetry during SONNE cruise SO202 (INOPEX) with links to multibeam raw data files

Multibeam data were measured during R/V SONNE cruise SO202 (INOPEX) along track lines of 6938 NM total length in the North Pacific and Bering Sea during transits and stationary work. Starting from Hokkaido (Japan) data were achieved east of the Kuril-Kamchatka Trench and south of the Aleutian Trench. The track crosses the Bowers Ridge, the continental margin of Alaska and the Umnak Plateau in the Bering Sea. Further data were gained in the North Pacific in the area of the Patton Seamounts, Gibson Seamount, Hess Rise and Shatsky Rise. The multibeam sonar system Simrad EM 120 from Kongsberg was operated using 191 beams and an aperture angle of 90° to 140° due to particular conditions. The refraction correction was achieved utilizing 6 CTD profiles measured during the cruise and one from cruise SO201. The quality of data might be reduced during bad weather periods. The dataset contains raw data that are not processed and thus may contain errors and blunders in depth and position.

Water temperature data from southern elephant seal from Marion Island with links to datasets

As the Antarctic Circumpolar Current crosses the South-West Indian Ocean Ridge, it creates an extensive eddy field characterised by high sea level anomaly variability. We investigated the diving behaviour of female southern elephant seals from Marion Island during their post-moult migrations in relation to this eddy field in order to determine its role in the animals' at-sea dispersal. Most seals dived within the region significantly more often than predicted by chance, and these dives were generally shallower and shorter than dives outside the eddy field. Mixed effects models estimated reductions of 44.33 ± 3.00 m (maximum depth) and 6.37 ± 0.10 min (dive duration) as a result of diving within the region, along with low between-seal variability (maximum depth: 5.5 % and dive duration: 8.4 %). U-shaped dives increased in frequency inside the eddy field, whereas W-shaped dives with multiple vertical movements decreased. Results suggest that Marion Island's adult female elephant seals' dives are characterised by lowered cost-of-transport when they encounter the eddy field during the start and end of their post-moult migrations. This might result from changes in buoyancy associated with varying body condition upon leaving and returning to the island. Our results do not suggest that the eddy field is a vital foraging ground for Marion Island's southern elephant seals. However, because seals preferentially travel through this area and likely forage opportunistically while minimising transport costs, we hypothesise that climate-mediated changes in the nature or position of this region may alter the seals' at-sea dispersal patterns.

Nd and Sr isotopic compositions and isotope dilution trace element concentrations from 7 sediment cores along the southeast Greenland margin

The Nd and Sr isotopic compositions of Quaternary glacial and glacimarine siliciclastic sediments deposited along the margin of southeast Greenland were determined to assess the roles of the Greenland, Iceland, and more distal ice sheets in delivering detritus to this portion of the northern North Atlantic. The isotopic compositions of detritus generated by portions of the southern Greenland Ice Sheet were defined through measurements of till and trough mouth fan sediments. Massive diamicts from the Scoresby Sund trough mouth fan show a restricted range of e-Nd (-11.8 to -16.6) and 87Sr/86Sr (0.7192-0.7246) consistent with their derivation from mixtures of sediments derived from Paleoproterozoic and/or Caledonian basement and Tertiary Greenland basalts. Further south at Kangerlussuaq, till isotopic compositions covary with the underlying basement type, with low e-Nd values in the inner fiord (-18.1) reflecting the erosion of the local Precambrian gneisses, but with higher e-Nd values (-2.3 to 2.5) found where the trough crosses East Greenland Tertiary basalts. Fine-grained (< 63 µm) sediments deposited along the southeast Greenland margin also show regular spatial isotopic variations. Ambient sediments and ice-rafted detritus in the southern Irminger Basin trend towards low e-Nd values (to ~ -28) and 87Sr/86Sr ratios (~ 0.711 to ~ 0.715) and are likely derived from proximal Archean gneisses of SE Greenland. Further north in the northern Irminger and Blosseville Basins, sediments trend toward much higher e-Nd (> -4) and low 87Sr/86Sr (< 0.709) reflecting a component derived from the local Iceland volcanic rocks and/or the East Greenland Tertiary basalts. In all three regions, the locally-derived detritus is intermixed with sediment with an intermediate e-Nd value (~ -10) and 87Sr/86Sr (~ 0.718) that was likely delivered by icebergs emanating from the Eurasian Ice Sheets and not from eastern Greenland. Deposition of glacial sediments from both proximal and distal (Eurasian) sources occurred adjacent to SE Greenland throughout the past 50 Ka, with periodic increases in IRD deposition at various times including those of Heinrich events 1, 2 and 4. These results suggest that at least the southern portions of the Greenland Ice Sheet experienced periodic instabilities during the Last Glacial period.

Age models for ODPO Leg 130 sites

The Neogene carbonate stratigraphy of five sites drilled on Ontong Java Plateau during Leg 130 reveals a number of patterns which are unexpected, and which we refer to as loss paradox, equatorial insensitivity, and climate paradox. They denote the following unresolved questions. 1 The loss of carbonate at depth (as derived from differences in accumulation rates) is much greater than suggested by the change in carbonate percentages (calculated under the assumption that carbonate dissolution is the cause of loss). This indicates an important role for redeposition processes, such as winnowing (bottom currents), sifting (resuspension and catabatic flow) and episodic sloughing or solifluction (presumably stimulated by earthquakes). 2 Accumulation rates are not markedly increased at the time a site crosses the equator. There are several possible reasons. Equatorial upwelling may be unimportant in controlling sedimentation rates this far in the western Pacific, or its output may be spread over a considerable distance from the equator. Alternatively, increased supply below the equator is compensated for by increased removal (e.g. from resuspension by bioturbation, combined with catabatic flow). It is conceivable that errors in the timescale could also produce the effect seen. 3 There is an overall tendency for agreement between the stratigraphic patterns of carbonate content and of accumulation rates, but neither pattern is readily explained by reference to changes in climate (represented by benthic delta18O) or in sea-level (as derived from sequence stratigraphy).

Projected near-future CO2 levels increase activity and alter defensive behaviours in the tropical squid Idiosepius pygmaeus

Carbon dioxide (CO2) levels projected to occur in the oceans by the end of this century cause a range of behavioural effects in fish, but whether other highly active marine organisms, such as cephalopods, are similarly affected is unknown. We tested the effects of projected future CO2 levels (626 and 956 µatm) on the behaviour of male two-toned pygmy squid, Idiosepius pygmaeus. Exposure to elevated CO2 increased the number of active individuals by 19-25% and increased movement (number of line-crosses) by nearly 3 times compared to squid at present-day CO2. Squid vigilance and defensive behaviours were also altered by elevated CO2 with >80% of individuals choosing jet escape responses over defensive arm postures in response to a visual startle stimulus, compared with 50% choosing jet escape responses at control CO2. In addition, more escape responses were chosen over threat behaviours in body pattern displays at elevated CO2 and individuals were more than twice as likely to use ink as a defence strategy at 956 µatm CO2, compared with controls. Increased activity could lead to adverse effects on energy budgets as well as increasing visibility to predators. A tendency to respond to a stimulus with escape behaviours could increase survival, but may also be energetically costly and could potentially lead to more chases by predators compared with individuals that use defensive postures. These results demonstrate that projected future ocean acidification affects the behaviours of a tropical squid species.

Swath sonar bathymetry during POLARSTERN cruise ANT-XXVI/2 (PS75) with links to multibeam raw data files

Multibeam data were collected during R/V Polarstern cruise ANT-XXVI/2 along track lines of about 9,270 NM total length along transits, survey profiles and during stationary work. Departing in Punta Arenas the ship headed for its first main working area, the Eltanin Impact Area. In the following the ship's track crosses Pacific Antarctic Ridge and the corresponding fracture zones several times before arriving in Wellington. The refraction correction was achieved utilizing CTD profiles or by the system's own cross fan calibration. The quality of data might be reduced during bad weather periods or adverse sea ice conditions. The dataset contains raw data that are not processed and thus may contain errors and blunders in depth and position.

Adaptation to enemy shifts: rapid resistance evolution to local Vibrio spp. in invasive Pacific oysters

One hypothesis for the success of invasive species is reduced pathogen burden, resulting from a release from infections or high immunological fitness (low immunopathology) of invaders. Despite of strong selection exerted on the host, the evolutionary response of invaders to newly acquired pathogens has rarely been considered. The two independent and genetically distinct invasions of the Pacific oyster Crassostrea gigas into the North Sea represent an ideal model system to study fast evolutionary responses of invasive populations. By exposing both invasion sources to ubiquitous and phylogenetically diverse pathogens (Vibrio spp.) we demonstrate that within a few generations hosts adapted to sympatric pathogen communities. However, this local adaptation only became apparent in selective environments, i.e. at elevated temperatures reflecting patterns of disease outbreaks in natural populations. Resistance against sympatric and allopatric Vibrio spp. strains was dominantly inherited in crosses between both invasion sources, resulting in an overall higher resistance of admixed individuals than pure lines. Therefore we suggest that a simple genetic resistance mechanism of the host is matched to a common virulence mechanism shared by local Vibrio strains. This combination might have facilitated a fast evolutionary response that can explain another dimension of why invasive species can be so successful in newly invaded ranges.

Physical oceanography and current measurements in the Filchner Depression (Weddell Sea, Antarctica) during Ernest Shackleton cruise ES060

Cold and dense ice shelf water (ISW) emerging from the Filchner-Ronne Ice Shelf cavity in the southwestern Weddell Sea flows northward through the Filchner Depression to eventually descend the continental slope and contribute to the formation of bottom water. New ship-born observations of hydrography and currents from Filchner Depression in January 2013 suggest that the northward flow of ISW takes place in a middepth jet along the eastern flank of the depression, thus questioning the traditional view with outflow along the western flank. This interpretation of the data is supported by results from a regional numerical model, which shows that ISW flowing northward along the eastern coast of Berkner Island turns eastward and crosses the depression to its eastern side upon reaching the Filchner ice front. The ice front represents a sudden change in the thickness of the water column and thus a potential vorticity barrier. Transport estimates of northward ISW flux based on observations ranges from 0.2 to 1.0 Sv.

Sedimentology of the southern Bellingshausen Sea

A major trough ('Belgica Trough') eroded by a palaeo-ice stream crosses the continental shelf of the southern Bellingshausen Sea (West Antarctica) and is associated with a trough mouth fan ('Belgica TMF') on the adjacent continental slope. Previous marine geophysical and geological studies investigated the bathymetry and geomorphology of Belgica Trough and Belgica TMF, erosional and depositional processes associated with bedform formation, and the temporal and spatial changes in clay mineral provenance of subglacial and glaciomarine sediments. Here, we present multi-proxy data from sediment cores recovered from the shelf and uppermost slope in the southern Bellingshausen Sea and reconstruct the ice-sheet history since the last glacial maximum (LGM) in this poorly studied area of West Antarctica. We combined new data (physical properties, sedimentary structures, geochemical and grain-size data) with published data (shear strength, clay mineral assemblages) to refine a previous facies classification for the sediments. The multi-proxy approach allowed us to distinguish four main facies types and to assign them to the following depositional settings: 1) subglacial, 2) proximal grounding-line, 3) distal sub-ice shelf/subsea ice, and 4) seasonal open-marine. In the seasonal open-marine facies we found evidence for episodic current-induced winnowing of near-seabed sediments on the middle to outer shelf and at the uppermost slope during the late Holocene. In addition, we obtained data on excess 210Pb activity at three core sites and 44 AMS 14C dates from the acid-insoluble fraction of organic matter (AIO) and calcareous (micro-)fossils, respectively, at 12 sites. These chronological data enabled us to reconstruct, for the first time, the timing of the last advance and retreat of the West Antarctic Ice Sheet (WAIS) and the Antarctic Peninsula Ice Sheet (APIS) in the southern Bellingshausen Sea. We used the down-core variability in sediment provenance inferred from clay mineral changes to identify the most reliable AIO 14C ages for ice-sheet retreat. The palaeo-ice stream advanced through Belgica Trough after ~36.0 corrected 14C ka before present (B.P.). It retreated from the outer shelf at ~25.5 ka B.P., the middle shelf at ~19.8 ka B.P., the inner shelf in Eltanin Bay at ~12.3 ka B.P., and the inner shelf in Ronne Entrance at ~6.3 ka B.P.. The retreat of the WAIS and APIS occurred slowly and stepwise, and may still be in progress. This dynamical ice-sheet behaviour has to be taken into account for the interpretation of recent and the prediction of future mass-balance changes in the study area. The glacial history of the southern Bellingshausen Sea is unique when compared to other regions in West Antarctica, but some open questions regarding its chronology need to be addressed by future work.

30 years of upper air soundings on board of R/V POLARSTERN (1982-12-29 to 2012-11-25)

The research vessel and supply icebreaker POLARSTERN is the flagship of the Alfred-Wegener-Institut in Bremerhaven (Germany) and one of the infrastructural pillars of German Antarctic research. Since its commissioning in 1982, POLARSTERN has conducted 30 campaigns to Antarctica (157 legs, mostly austral summer), and 29 to the Arctic (94 legs, northern summer). Usually, POLARSTERN is more than 300 days per year in operation and crosses the Atlantic Ocean in a meridional section twice a year. The first radiosonde on POLARSTERN was released on the 29th of December 1982, two days after POLARSTERN started on its maiden voyage to the Antarctic. And these daily soundings have continued up to the present. Due to the fact that POLARSTERN has reliably and regularly been providing upper air observations from data sparse regions (oceans and polar regions), the radiosonde data are of special value for researchers and weather forecast services alike. In the course of 30 years (1982-12-29 to 2012-11-25) a total of 12378 radiosonde balloons were started on POLARSTERN. All radiosonde data can now be found here. Each dataset contains the directly measured parameters air temperature, relative humidity and air pressure, and the derived altitude, wind direction and wind speed. 432 datasets additionally contain ozone measurements.

Physical properties of sediment cores from the Iberian Continental Slope

Climatic changes cause alterations in circulation patterns of the world oceans. The highly saline Mediterranean Outflow Water (MOW), built within the Mediterranean Sea crosses the Strait of Gibraltar in westerly directions, turning north-westward to stick to the Iberian Slope within 600-1500m water depths. Circulation pattern and current speed of the MOW are strongly influenced by climatically induced variations and thus control sedimentation processes along the South- and West - Iberian Continental Slope. Sedimentation characteristics of the investigated area are therefore suitable to reconstruct temporal hydrodynamic changes of the MOW. Detailed investigations on the silt-sized grain distribution, physical properties and hydroacoustic data were performed to recalculate paleo-current-velocities and to understand the sedimentation history in the Golf of Cadiz and the Portuguese Continental Slope. A time model based on d18Odata and 14C-datings of planktic foraminifera allowed the stratigraphical classification of the core material and thus the dating of the current induced sediment layers showing the variations of paleo-current intensities. The evaluation and interpretation of the gathered data sets enabled us to reconstruct lateral and temporal sedimentation patterns of the MOW for the Holocene and the late Pleistocene, back to the Last Glacial Maximum (LGM).