Satellite image map of King George Island, Antarctica

A new topographic database for King George Island, one of the most visited areas in Antarctica, is presented. Data from differential GPS surveys, gained during the summers 1997/98 and 1999/2000, were combined with up to date coastlines from a SPOT satellite image mosaic, and topographic information from maps as well as from the Antarctic Digital Database. A digital terrain model (DTM) was generated using ARC/INFO GIS. From contour lines derived from the DTM and the satellite image mosaic a satellite image map was assembled. Extensive information on data accuracy, the database as well as on the criteria applied to select place names is given in the multilingual map. A lack of accurate topographic information in the eastern part of the island was identified. It was concluded that additional topographic surveying or radar interferometry should be conducted to improve the data quality in this area. In three case studies, the potential applications of the improved topographic database are demonstrated. The first two examples comprise the verification of glacier velocities and the study of glacier retreat from the various input data-sets as well as the use of the DTM for climatological modelling. The last case study focuses on the use of the new digital database as a basic GIS (Geographic Information System) layer for environmental monitoring and management on King George Island.

PumaPlex 1.0: Data generated during the development of the SNP markers and SNP genotypes of pumas

Pumas are one of the most studied terrestrial mammals because of their widespread distribution, substantial ecological impacts, and conflicts with humans. Extensive efforts, often employing genetic methods, are undertaken to manage this species. However, the comparison of population genetic data is difficult because few of the microsatellite loci chosen are shared across research programs. Here, we describe the development of PumaPlex, a high-throughput assay to genotype 25 single nucleotide polymorphisms in pumas. We validated PumaPlex in more than 700 North American pumas (Puma concolor couguar), and demonstrated its ability to generate reproducible genotypes and accurately identify individuals. Furthermore, we compared PumaPlex with traditional genotyping of 12 microsatellite loci in fecal DNA samples and found that PumaPlex produced significantly more genotypes with fewer false alleles. PumaPlex promotes the cross-laboratory comparison of genotypes, is easily expandable in the future, and is a valuable tool for the genetic monitoring and management of North American puma populations.