Oxygen isotopic measurements from Holcene of sediment core MD02-2529, tropical pacific

Changes in El Niño-Southern Oscillation (ENSO) variability are difficult to extract from paleoceanographic reconstructions because they are superimposed on changes in seasonal variability that modulate the first-order climate signal. Here we address this problem by reconstructing thermocline structure from a marine sediment core retrieved from the eastern equatorial Pacific. At the core location, changes in hydrologic parameters within the thermocline are linked to ENSO activity, with a reduced influence of seasonal variability compared to surface waters. We performed repeated isotopic analyses (d18O) on single specimens of the thermocline-dwelling planktonic foraminifera Neogloboquadrina dutertrei at several targeted time periods over the last 50 ka to extract the total thermocline variance, a parameter supposed to reveal changes in ENSO. No fundamental changes in amplitude and frequency of the events were detected despite differences in climatic background. However, our data suggest that long-term variations in the thermocline variability occurred over the last 50 ka, with the highest and lowest ENSO activities occurring during the last glacial period and the Last Glacial Maximum, respectively.

Seawater carbonate chemistry, Mg/Ca, Sr/Ca, oxygen production and calcification rate during experiments with coral Pocillopora damicornis, 2011

The Sr/Ca of aragonitic coral skeletons is a commonly used palaeothermometer. However skeletal Sr/Ca is typically dominated by weekly-monthly oscillations which do not reflect temperature or seawater composition and the origins of which are currently unknown. To test the impact of transcellular Ca2+ transport processes on skeletal Sr/Ca, colonies of the branching coral, Pocillopora damicornis, were cultured in the presence of inhibitors of Ca-ATPase (ruthenium red) and Ca channels (verapamil hydrochloride). The photosynthesis, respiration and calcification rates of the colonies were monitored throughout the experiment. The skeleton deposited in the presence of the inhibitors was identified (by 42Ca spike) and analysed for Sr/Ca and Mg/Ca by secondary ion mass spectrometry. The Sr/Ca of the aragonite deposited in the presence of either of the inhibitors was not significantly different from that of the solvent (dimethyl sulfoxide) control, although the coral calcification rate was reduced by up to 66% and 73% in the ruthenium red and verapamil treatments, respectively. The typical precision (95% confidence limits) of mean Sr/Ca determinations within any treatment was <±1% and differences in skeletal Sr/Ca between treatments were correspondingly small. Either Ca-ATPase and Ca channels transport Sr2+ and Ca2+ in virtually the same ratio in which they are present in seawater or transcellular processes contribute little Ca2+ to the skeleton and most Ca is derived from seawater transported directly to the calcification site. Variations in the activities of Ca-ATPase and Ca-channels are not responsible for the weekly-monthly Sr/Ca oscillations observed in skeletal chronologies, assuming that the specificities of Ca transcellular transport processes are similar between coral genera.

Stable oxygen isotope data and geophysical measurements from Red Sea of core M174/Kl11

To settle debate on the timing of sea level fluctuations during marine isotope stage (MIS) 3, we present records of d18O ruber (sea level proxy) and magnetic susceptibility from the same samples within the single sediment archive (i.e., "coregistered") of central Red Sea core GeoTü-KL11. Core-scanning X-ray fluorescence and environmental magnetic data establish the suitability of magnetic susceptibility as a proxy for eolian dust content in Red Sea sediments. The eolian dust data record similar variability as Greenland d18O ice during early to middle MIS 3, in agreement with previous observations that regional Arabian Sea climate fluctuated with a timing similar to that of Greenland climate variations. In contrast, the sea level record fluctuates with a timing similar to that of Antarctic-style climate variations. The coregistered nature of the two records in core KL11 unambiguously reveals a distinct offset in the phase relationship between sea level and eolian dust fluctuations. The results confirm that sea level rises, indicated by shifts in Red Sea d18O ruber to lighter values, occurred during cold episodes in Greenland during early to middle MIS 3. This indicates that the amplitudes of the reconstructed MIS 3 sea level fluctuations would not be reduced by inclusion of regional climate fluctuations in the Red Sea sea level method. Our analysis comprehensively supports our earlier conclusions of large-amplitude sea level variations during MIS 3 with a timing that is strongly similar to Antarctic-style climate variations.

Calcification repsonse of m,arione bivalves to changed carbonate chemistry

Bivalve calcification, particularly of the early larval stages, is highly sensitive to the change in ocean carbonate chemistry resulting from atmospheric CO2 uptake. Earlier studies suggested that declining seawater [CO32-] and thereby lowered carbonate saturation affect shell production. However, disturbances of physiological processes such as acid-base regulation by adverse seawater pCO2 and pH can affect calcification in a secondary fashion. In order to determine the exact carbonate system component by which growth and calcification are affected it is necessary to utilize more complex carbonate chemistry manipulations. As single factors, pCO2 had no effects and [HCO3-] and pH had only limited effects on shell growth, while lowered [CO32-] strongly impacted calcification. Dissolved inorganic carbon (CT) limiting conditions led to strong reductions in calcification, despite high [CO32-], indicating that [HCO3-] rather than [CO32-] is the inorganic carbon source utilized for calcification by mytilid mussels. However, as the ratio [HCO3-] / [H+] is linearly correlated with [CO32-] it is not possible to differentiate between these under natural seawater conditions. An equivalent of about 80 µmol kg-1 [CO32-] is required to saturate inorganic carbon supply for calcification in bivalves. Below this threshold biomineralization rates rapidly decline. A comparison of literature data available for larvae and juvenile mussels and oysters originating from habitats differing substantially with respect to prevailing carbonate chemistry conditions revealed similar response curves. This suggests that the mechanisms which determine sensitivity of calcification in this group are highly conserved. The higher sensitivity of larval calcification seems to primarily result from the much higher relative calcification rates in early life stages. In order to reveal and understand the mechanisms that limit or facilitate adaptation to future ocean acidification, it is necessary to better understand the physiological processes and their underlying genetics that govern inorganic carbon assimilation for calcification.