Anaerobic methanotrophic archaea-2 and Desulfosarcina/Desulfococcus group in sediments at different stations

The anaerobic oxidation of methane (AOM) with sulfate as terminal electron acceptor is mediated by consortia of methanotrophic archaea (ANME) and sulfate-reducing bacteria (SRB). In sediment samples from Hydrate Ridge, the Isis Mud Volcano and the Gulf of Mexico, DSS cells accounted for 3-6% of all DAPI-stained single cells. Out of these, 8-17% were labelled with probe SEEP1a-1441. This translated into relative abundances of single SEEP-SRB1a cells of 0.3% to 0.7%. Contrastingly, in a sediment sample from the Gullfaks oil field, DSS cells accounted for 18% and SEEP-SRB1a for 9% of all single cells. This sediment sample also featured an unusually high abundance of single ANME-2 cells and only very few ANME-2/DSS aggregates in comparison with other AOM habitats. Considering also the nature of the sample, it is likely that the high number of single ANME-2 and SEEP-SRB1a cells were an artifact of sample preparation. Here, harsher sonication was required to remove the microorganisms from coarse sand prior to CARD-FISH analysis.

(Table 6a) 137Cs concentrations in waters of the Sea of Japan in 1994 and in 2002

Sulfide, S°, and thiosulfate were determined in waters of the Baltic Sea. Microquantities of these compounds were observed in oxic waters. Concentration levels of reduced sulfur compounds in Baltic oxic waters were very close to levels of the Black Sea oxic zone. Thiosulfate and S° were predominate compounds in oxic water whereas sulfide was a predominant compound Baltic waters high in hydrogen sulfide. Conclusion was made that during sedimentation in oxic waters anaerobic microorganisms along with aerobic bacteria take part in mineralization of organic matter. They exist on surfaces and in microniches of particles of organic detritus.

(Table 6b) 137Cs concentrations in surface layer bottom sediments of the Sea of Japan in 1994 and in 2002

Sulfide, S°, and thiosulfate were determined in waters of the Baltic Sea. Microquantities of these compounds were observed in oxic waters. Concentration levels of reduced sulfur compounds in Baltic oxic waters were very close to levels of the Black Sea oxic zone. Thiosulfate and S° were predominate compounds in oxic water whereas sulfide was a predominant compound Baltic waters high in hydrogen sulfide. Conclusion was made that during sedimentation in oxic waters anaerobic microorganisms along with aerobic bacteria take part in mineralization of organic matter. They exist on surfaces and in microniches of particles of organic detritus.

Isotopes content, mineralogical composition, Uranium and Thorium contents and determined activity ratios from METEOR cruise M74/3 in 2007

Authigenic carbonate deposits have been sampled with the remotely operated vehicle 'MARUM-QUEST 4000 m' from five methane seeps between 731 and 1823 m water depth along the convergent Makran continental margin, offshore Pakistan (northern Arabian Sea). Two seeps on the upper slope are located within the oxygen minimum zone (OMZ; ca. 100 to 1100 m water depth), the other sites are situated in oxygenated water below the OMZ (below 1100 m water depth). The carbonate deposits vary with regard to their spatial extent, sedimentary fabrics, and associated seep fauna: Within the OMZ, carbonates are spatially restricted and associated with microbial mats, whereas in the oxygenated zone below the OMZ extensive carbonate crusts are exposed on the seafloor with abundant metazoans (bathymodiolin mussels, tube worms, galatheid crabs). Aragonite and Mg-calcite are the dominant carbonate minerals, forming common early diagenetic microcrystalline cement and clotted to radial-fibrous cement. The delta18O carbonate values range from 1.3 to 4.2 per mil V-PDB, indicating carbonate precipitation at ambient bottom-water temperature in shallow sediment depth. Extremely low delta13Ccarbonate values (as low - 54.6per mil V-PDB) point to anaerobic oxidation of methane (AOM) as trigger for carbonate precipitation, with biogenic methane as dominant carbon source. Prevalence of biogenic methane in the seepage gas is corroborated by delta13C methane values ranging from - 70.3 to - 66.7per mil V-PDB, and also by back-calculations considering delta 13C methane values of carbonate and incorporated lipid biomarkers.