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Here we report the amount of archaeal cardiolipins analogues in a cold seep off Pakistan (Makran accretionary prism). The published data (Yoshinaga et al., 2012) describes a series of tentatively identified cardiolipin analogues (dimeric phospholipids or bisphosphatidylglycerol), which represented 0.5% to 5% of total archaeal intact polar lipids.