(Table, page 13-59), Annotated record of the detailed examination of Mn deposits from Amphitrite Expedition stations

The cores described in this report were taken on AMPHITRITE Expedition in Decenber 1963 - February 1964 by Scripps Institution of Oceanography from, the R/V Argo. A total of 148 cores were recovered and are available at Scripps for sampling and study. The coring sites, all in the tropical central Pacific. The AMPHITRITE cores are here briefly described to identify visually distinct units based on lithology, color, texture, or other characteristic unique to an interval of sediment. For determination of lithology, the slides prepared from samples of the cores were examined microscopically in conjuction with the visual examination.

Heavy metal contents in Fe-Mn nodules and crusts from R/V Vityaz polygons and stations in the East Indian Ocean

Regional variations in abundance, morphology, and chemical composition of Fe-Mn nodules have a zonal character. Due to circumcontinental zonality of terrigenous sedimentation the main mass of the nodules occurs in the pelagic part of the ocean, in areas of minimal sedimentation rates. In spatial variations in morphology and chemical composition of the nodules the latitudinal zonality is very clear and associated with latitudinal changes in facial conditions of sedimentation. Elevated contents of Mn, Ni, and Cu and of Mn/Fe ratio occur in nodules from the radiolarian belt. Changes of chemical composition of the nodules with depth (vertical zonality of mineralization) are confirmed. Local variations in abundance, morphology and chemical composition of the nodules are caused by ruggedness of relief and depth variations, variations in sedimentation rate, age of ore formation, intensity of diagenetic redistribution of metals.

(Table 1, page 1157) DOWNWIND expedition bottom photo stations showing abundant manganese nodules

Manganese nodules were investigated during the Downwind Expedition, a part of the International Geophysical Year programme of the Scripps Institution of Oceanography of the University of California. Attempts were made to collect bottom photographs, cores and dredge hauls in the same areas, to measure the distribution at the surface and in depth, and to obtain large samples for physical and chemical analysis.

Annotated record of the detailed examination of Mn deposits from DODO Expedition stations

The cores and dredges described in this report were taken on the DODO Expedition in May 1964 until December 1964 by the Scripps Institution of Oceanography from the R/V Argo. A total of 290 cores and dredges were recovered and are available at Scripps for sampling and study.

Benthic anammox and dentrification rates measured in a slurry incubation experiments at four different stations in the Arabian Sea off Pakistan during the METEOR cruise M74/2 in 2007

Vertical distributions of benthic denitrification and anammox rates within the sediment were estimated from slurry incubation experiments. Rates were used to calculate the contribution of anammox and denitrification to the total N-loss. Briefly, MUC sediment cores were sliced in 2 cm intervals and the sediment was diluted and incubated with degassed bottom water in a gas tight bag. After pre-incubating the bags for 2 h, 15N-labeled substrates were injected into the bags and the slurries were thoroughly mixed. Incubations were performed in the dark at in situ temperatures. The N2 isotope ratio (28N2, 29N2, and 30N2) was determined by gas chromatography-isotopic ratio mass spectrometry (VG Optima, Micromass) and calculated according to Kuypers et al. (2005) and Holtappels et al. (2011), respectively.Furthermore, total organic carbon and nitrogen concentrations were measured of core sediment layers corresponding to those used for rate measurements. Concentrations of organic carbon and nitrogen were determined by combustion/gas chromatography (Carlo Erba NA-1500 CNS analyzer) of dried sediment samples after acidification. The same sediment layer were also used to extract nucleic acids. The concentrations of the DNA in the samples were measured spectrophotometrically with a NanoDrop instrument (Thermo Fisher Scientific Inc.). The biomarker functional gene nirS, encoding the cd1-containing nitrite reductase, for both denitrifiers and marine anammox bacteria were quantified with real-time PCR, using the primers cd3aF/R3cd (5'-GTSAACGTSAAGGARACSGG-3' (Michotey et al., 2000)/5'-GASTTCGGRTGSGTCTTGA-3'; Throback et al., 2004) and Scnir372F/Scnir845R (5'-TGTAGCCAGCATTGTAGCGT-3'/5'-TCAAGCCAGACCCATTTGCT-3'; Lam et al., 2009).