Gene expression changes in the coccolithophore Emiliania huxleyi after 500 generations of selection to ocean acidification

Coccolithophores are unicellular marine algae that produce biogenic calcite scales and substantially contribute to marine primary production and carbon export to the deep ocean. Ongoing ocean acidification particularly impairs calcifying organisms, mostly resulting in decreased growth and calcification. Recent studies revealed that the immediate physiological response in the coccolithophore Emiliania huxleyi to ocean acidification may be partially compensated by evolutionary adaptation, yet the underlying molecular mechanisms are currently unknown. Here, we report on the expression levels of 10 candidate genes putatively relevant to pH regulation, carbon transport, calcification and photosynthesis in E. huxleyi populations short-term exposed to ocean acidification conditions after acclimation (physiological response) and after 500 generations of high CO2 adaptation (adaptive response). The physiological response revealed downregulation of candidate genes, well reflecting the concomitant decrease of growth and calcification. In the adaptive response, putative pH regulation and carbon transport genes were up-regulated, matching partial restoration of growth and calcification in high CO2-adapted populations. Adaptation to ocean acidification in E. huxleyi likely involved improved cellular pH regulation, presumably indirectly affecting calcification. Adaptive evolution may thus have the potential to partially restore cellular pH regulatory capacity and thereby mitigate adverse effects of ocean acidification.

(Figure 3d) Export production reconstruction based on planktonic foraminiferal sampled in surface sediments

To reconstruct Export Productivity (Pexp), 27 taxonomic categories of the planktonic foraminifera census data were used with the modern analog technique SIMMAX 28 (Pflaumann et al., 1996, doi:10.1029/95PA01743; 2003, doi:10.1029/2002PA000774). To the 26 taxonomic groups widely used and listed in Kucera et al. (2005, doi:10.1016/j.quascirev.2004.07.014), Turborotalita humilis was added in our calibration as it is associated with the PCC source region (Meggers et al., 2002, doi:10.1016/S0967-0645(02)00103-0). The modern analog file is based on the Iberian margin database (Salgueiro et al., 2008, doi:10.1016/j.marmicro.2007.09.003) combined with the North Atlantic surface samples used by the MARGO project (Kucera et al., 2005). This results in a total of 999 analogs for Pexp. Modern oceanic primary productivity (PP) is obtained for each site by averaging 12 monthly primary productivity values for a 8-year period (1978-1986) that were estimated from satellite color data (CZCS) and gridded at 0.5° latitude - longitude fields (Antoine et al., 1996, doi:10.1029/95GB02832). Export Productivity (Pexp) was calculated from the PP values following the empirical relationship Pexp = PP**2/400 for primary production below 200 gC/m**2/yr, and Pexp = PP/2 for primary production above 200 gC/m2/yr (Eppley and Peterson, 1979, doi:10.1038/282677a0; Sarnthein et al., 1988, doi:10.1029/PA003i003p00361). The residuals gives the differences between satellite based Pexp and foraminiferal Pexp.