The glacier inventory of Salzburg, Austria, 2007/2009

A third glacier inventory (GI3) is presented for the province of Salzburg where 173 glaciers are located in the seven mountain ranges: Ankogel (47°4'N, 13°14'E), Glockner, Granatspitz, Sonnblick (Goldberg), Hochkönig, Venediger and Zillertal (47°8'N, 12°7'E). The basis for the new GI3 are orthophotos of 2007 and 2009 and the digital elevation model (DEM) of the southern part of Salzburg. On the basis of former inventories, area- and volume changes have been calculated. The biggest relative loss of glacier area per mountain range was found in the Ankogel range and on Hochkönig as a result of the disrupted structure of their small and thin glaciers. In terms of absolute values, the largest changes took place in the Glockner- and Venediger range with an area loss of -10.1 km**2 and -9.7 km**2 during the period between GI1 (1969) and GI3 (2007/2009), respectively. Volume changes have been calculated for nearly half of the glacier area in Salzburg, where DEMs were available. The Glockner, Granatspitz and Sonnblick mountain ranges showed a volume loss of -0.481 km**3 which corresponds to a mean thickness change of -10.5 m. An extrapolation of these changes to all of the 173 glaciers in Salzburg results in a loss of about 1.04 km**3 between GI1 and GI3 and 0.44 km**3 between GI2 and GI3. Overall annual changes in the province of Salzburg between GI2 and GI3 were higher than between GI1 and GI2 and show likewise changes such as those of Tyrol.

Grazing rate of microzooplankton measured experimentally in the North Atlantic during the Maria S. Merian cruise MSM26, spring 2013

The microzooplankton grazing dilution experiments were conducted at stations 126, 127, 131 and 133-137, following Landry & Hassett (1982). Seawater samples (whole seawater - WSW) were taken via Niskin bottles mounted on to a CTD Rosette out of the chlorophyll maximum at each station. Four different dilution levels were prepared with WSW and GF/F filtered seawater - 100% WSW, 75% WSW, 50% WSW and 25% WSW. The diluted WSW was filled in 2.4 L polycarbonate bottles (two replicates for every dilution level). Three subsamples (250 - 500 mL depending on in situ chlorophyll) of the 100% WSW were filtered on to GF/F filters (25 mm diameter) and chlorophyll was extracted in 5 mL 96% ethanol for 12-24 hours. Afterwards it was measured fluorometrically before and after the addition of HCl with a Turner fluorometer according to Jespersen and Christoffersen (1987) on board of the ship. In addition, one 250 mL subsample of the 100% WSW was fixed in 2% Lugol (final concentration), to determine the microzooplankton community when back at the Institute for Hydrobiology and Fisheries Science in Hamburg. Also, one 50 mL subsample of the 100% WSW was fixed in 1 mL glutaraldehyde, to quantify bacteria abundance. The 2.4 L bottles were put in black mesh-bags, which reduced incoming radiation to approximately 50% (to minimize chlorophyll bleaching). The bottles were incubated for 24 hours in a tank on deck with flow-through water, to maintain in situ temperature. An additional experiment was carried out to test the effect of temperature on microzooplankton grazing in darkness. Therefore, 100% WSW was incubated in the deck tank and in two temperature control rooms of 5 and 15°C in darkness (two bottles each). The same was done with bottles where copepods were added (five copepods of Calanus finmarchicus in each bottle; males and females were randomly picked and divided onto the bottles). In addition, two 100% WSW bottles with five copepods each were incubated at in situ temperature at 100% light level (without mesh-bags). All experiments were incubated for 24 hours and afterwards two subsamples of each bottle were filtered on to GF/F filters (25 mm diameter); 500 - 1000 mL depending on in situ chlorophyll. One 250 mL subsample of one of the two replicates of each dilution level and each additional experiment (temperature and temperature/copepods) was fixed in 5 mL lugol for microzooplankton determination. One 50 mL subsample of one of the two 100% WSW bottles as well as of one of the additional experiments without copepods was fixed in 1 mL glutaraldehyde for bacteria determination later on. Copepods were fixed in 4% formaldehyde for length measurements and sex determination.