Abundance and biomass of meiobenthos in the Black Sea during Mare Nigrum cruise S-RO1

Dataset containing meiobenthos data for samples collected during the September 2008 Sesame Cruise in the North-West Black Sea on board of the Romanian R/V Mare Nigrum. Meiobenthos samples were collected in 5 stations, using a multicorer MARK II-400. The dataset includes 5 samples analysed for meiobenthos species composition, abundance and biomass. The entire washed sample was analyzed under the binocular stereomicroscope. Meiobenthic species were identified and enumerated; some meiobenthic species were identified and enumerated only at higher taxonomic level. Taxonomic identification was done at GEOECOMAR.

Abundance of phytoplankton in the Black Sea during the Bilim 2 cruise in April 2008

The dataset is composed of 46 samples from 9 stations. The phytoplankton samples were collected by 5l Niskin bottles attached to the CTD system. The sampling depths were selected according to the CTD profiles and the in situ fluorometer readings. The samples (50 ml sea water) were preserved with prefiltered (0.2 micron) glutardialdehyde solution (1.5 ml of commercial glutardialdehyde (25%)) into dark colored glass bottles. Preserved samples were poured into 10 or 25 ml settling chambers (Hydro-Bios) for cells to settle on the bottom over a day. Species identification and enumeration were done under an inverted microscope (Olympus IX71). At least 400 specimen were tried to be counted in each sample.

Abundance and biomass of phytoplankton in the western part of the Black Sea during the R/V Akademik cruise Ak082001 in August 2001

The dataset is composed of 41 samples from 10 stations. The phytoplankton samples were collected by 5l Niskin bottles attached to the CTD system. The sampling depths were selected according to the CTD profile and the in situ fluorometer readings: surface, temperature, salinity and fluorescence gradients and 1 m above the bottom. At some stations phytoplankton net samples (20 µm mesh-size) were collected to assist species biodiversity examination. The samples (1l sea water) were preserved in 4% buffered to pH 8-8.2 with disodiumtetraborate formaldehyde solution and stored in plastic containers. On board at each station few live samples were qualitatively examined under microscope for preliminary analysis of taxonomic composition and dominant species. The taxon-specific phytoplankton abundance samples were concentrated down to 50 cm**3 by slow decantation after storage for 20 days in a cool and dark place. The species identification was done under light microscope OLIMPUS-BS41 connected to a video-interactive image analysis system at magnification of the ocular 10X and objective - 40X. A Sedgwick-Rafter camera (1ml) was used for counting. 400 specimen were counted for each sample, while rare and large species were checked in the whole sample (Manual of phytoplankton, 2005). Species identification was mainly after Carmelo T. (1997) and Fukuyo, Y. (2000). Total phytoplankton abundance was calculated as sum of taxon-specific abundances. Total phytoplankton biomass was calculated as sum of taxon-specific biomasses. The cell biovolume was determined based on morpho-metric measurement of phytoplankton units and the corresponding geometric shapes as described in detail in (Edier, 1979).

Abundance of mesozooplankton in the western part of the Black Sea during Mare Nigrum cruise S-RO1

The SESAME dataset contains mesozooplankton data collected during April 2008 in the North-Western part of Black Sea (between 44°46' N and 42°29'N latitude and 28°64'E and 30°59'E longitude). Mesozooplankton sampling was undertaken at 9 stations where samples were collected using a Hensen net in the 0-10, 10-25, 25-50, 50-100, 100-150, 150-200 m layer. The dataset includes 29 samples analysed for mesozooplankton species composition and abundance. The entire sample or an aliquot (1/2 to 1/4) was analyzed under the binocular microscope. Calculations of zooplankton abundance are made by the following formulae, in accordance with the Report of the third ICES/HELCOM workshop on quality assurance of Biological measurements Warnemünde, Germany, 1996. M - number of counted specimens (ind.), Vf - volume of filtrated water (m³), and K - counted part of sample. (http://www2008.io-warnemuende.de/research/helcom_zp/documents/qa_zp_part.pdf)

Abundance and biomass of phytoplankton in the western part of the Black Sea during Branimir Ormanov cruise BO092000 in September 2000

The samples were concentrated down to 50 cm**3 by slow decantation after storage for 20 days in a cool and dark place. The species identification was done under light microscope OLIMPUS-BS41 connected to a video-interactive image analysis system at magnification of the ocular 10X and objective - 40X. A Sedgwick-Rafter camera (1ml) was used for counting. 400 specimen were counted for each sample, while rare and large species were checked in the whole sample (Manual of phytoplankton, 2005). Species identification was mainly after Carmelo T. (1997) and Fukuyo, Y. (2000). Taxon-specific phytoplankton abundance and biomass were analysed by Moncheva S., B. Parr, 2005. Manual for Phytoplankton Sampling and Analysis in the Black Sea. The cell biovolume was determined based on morpho-metric measurement of phytoplankton units and the corresponding geometric shapes as described in detail in (Edier, 1979).