Localization of ion-regulatory epithelia in embryos and hatchlings of two cephalopods

The tissue distribution and ontogeny of Na+/K+-ATPase has been examined as an indicator for ion-regulatory epithelia in whole animal sections of embryos and hatchlings of two cephalopod species: the squid Loligo vulgaris and the cuttlefish Sepia officinalis. This is the first report of the immunohistochemical localization of cephalopod Na+/K+-ATPase with the polyclonal antibody alpha (H-300) raised against the human alpha1-subunit of Na+/K+-ATPase. Na+/K+-ATPase immunoreactivity was observed in several tissues (gills, pancreatic appendages, nerves), exclusively located in baso-lateral membranes lining blood sinuses. Furthermore, large single cells in the gill of adult L. vulgaris specimens closely resembled Na+/K+-ATPase-rich cells described in fish. Immunohistochemical observations indicated that the amount and distribution of Na+/K+-ATPase in late cuttlefish embryos was similar to that found in juvenile and adult stages. The ion-regulatory epithelia (e.g., gills, excretory organs) of the squid embryos and paralarvae exhibited less differentiation than adults. Na+/K+-ATPase activities for whole animals were higher in hatchlings of S. officinalis (157.0 ± 32.4 µmol/g FM/h) than in those of L. vulgaris (31.8 ± 3.3 µmol/g FM/h). S. officinalis gills and pancreatic appendages achieved activities of 94.8 ± 18.5 and 421.8 ± 102.3 µmol ATP/g FM/h, respectively. High concentrations of Na+/K+-ATPase in late cephalopod embryos might be important in coping with the challenging abiotic conditions (low pH, high pCO2) that these organisms encounter inside their eggs. Our results also suggest a higher sensitivity of squid vs. cuttlefish embryos to environmental acid-base disturbances.

Aboveground community and species-specific plant biomass from the Jena Experiment (Main Experiment, year 2008)

This data set contains aboveground community biomass (Sown plant community, Weed plant community, Dead plant material, and Unidentified plant material; all measured in biomass as dry weight) and species-specific biomass from the sown species of the main experiment plots of a large grassland biodiversity experiment (the Jena Experiment; see further details below). In the main experiment, 82 grassland plots of 20 x 20 m were established from a pool of 60 species belonging to four functional groups (grasses, legumes, tall and small herbs). In May 2002, varying numbers of plant species from this species pool were sown into the plots to create a gradient of plant species richness (1, 2, 4, 8, 16 and 60 species) and functional richness (1, 2, 3, 4 functional groups). Plots were maintained by bi-annual weeding and mowing. Aboveground community biomass was harvested twice in 2008 just prior to mowing (during peak standing biomass in early June and in late August) on all experimental plots of the main experiment. This was done by clipping the vegetation at 3 cm above ground in three rectangles of 0.2 x 0.5 m per large plot. The location of these rectangles was assigned prior to each harvest by random selection of coordinates within the core area of the plots (i.e. the central 10 x 15 m). The positions of the rectangles within plots were identical for all plots. The harvested biomass was sorted into categories: individual species for the sown plant species, weed plant species (species not sown at the particular plot), detached dead plant material (i.e., dead plant material in the data file), and remaining plant material that could not be assigned to any category (i.e., unidentified plant material in the data file). All biomass was dried to constant weight (70°C, >= 48 h) and weighed. Sown plant community biomass was calculated as the sum of the biomass of the individual sown species. The data for individual samples and the mean over samples for the biomass measures on the community level are given. Overall, analyses of the community biomass data have identified species richness as well as functional group composition as important drivers of a positive biodiversity-productivity relationship.

Pollen analysis of a peat profile from the Rieseberger Moor, Lower Saxony, Germany

The Rieseberger Moor is a fen, 145 hectares in size, situated about 20 km east of Brunswick (Braunschweig), Lower Saxony, Germany. Peat was dug in the fen - with changing intensity - since the mid-18th century until around AD 1955. According to Schneekloth & Schneider (1971) the remaining peat (fen and wood peat) is predominantly 1.5 to 2 m thick (maximum 2.7 m). Part of the fen - now a nature reserve (NSG BR 005) - is wooded (Betula, Salix, Alnus). For more information on the Rieseberger Moor see http://de.wikipedia.org/wiki/Rieseberger_Moor. Willi Selle was the first to publish pollen diagrams from this site (Selle 1935, profiles Rieseberger Torfmoor I and II). This report deals with a 2.2 m long profile from the wooded south-eastern part of the fen consisting of strongly decomposed fen peat taken A.D. 1965 and studied by pollen analysis in the same year. The peat below 1.45 m contained silt and clay, samples 1.48 and 1.58 m even fine sand. These samples had to be treated with HF (hydrofluoric acid) in addition to the treatment with hot caustic potash solution. The coring ended in sandy material. The new pollen data reflect the early part of the known postglacial development of the vegetation of this area: the change from a birch dominated forest to a pine forest and the later spreading of Corylus and of the thermophilous deciduous tree genera Quercus, Ulmus, Tilia and Fraxinus followed by the expansion of Alnus. The new data are in agreement with Selle's results, except for Alnus, which in Selle's pollen diagram II shows high values (up to 42% of the arboreal pollen sum) even in samples deposited before Corylus and Quercus started to spread. On contrary the new pollen diagram shows that alder pollen - although present in all samples - is frequent in the three youngest pollen spectra only. A period with dominating Alnus as seen in the uppermost part of Selle's pollen diagrams is missing. The latter is most likely the result of peat cutting at the later coring site, whereas the early, unusually high alder values of Selle's pollen study are probably caused by contamination of the pollen samples with younger peat. Selle took peat samples usually with a "Torfbohrer" (= Hiller sampler). This side-filling type of sampler with an inner chamber and an outer loose jacket offers - if not handled with appropriate care - ample opportunities to contaminate older peat with carried off younger material. Pollen grains of Fagus (2 % of the arboreal pollen sum) were found in two samples only, namely in the uppermost samples of the new profile (0.18 m) and of Selle's profile I (0.25 m). If this pollen is autochthonous, with other words: if this surface-near peat was not disturbed by human activities, the Fagus pollen indicates an Early Subboreal age of this part of the profile. The accumulation of the Rieseberg peat started during the Preboreal. Increased values of Corylus, Quercus and Ulmus indicate that sample 0.78 m of the new profile is the oldest Boreal sample. The high Alnus values prove the Atlantic age of the younger peat. Whether Early Subboreal peat exists at the site is questionable, but evidently none of the three profiles reaches to Late Subboreal time, when Fagus spread in the region. Did peat-growth end during the Subboreal? Did younger peat exist, but got lost by peat cutting or has younger peat simply not yet been found in the Rieseberg fen? These questions cannot be answered with this study. The temporary decline of the curve of Pinus for the benefit of Betula during the Preboreal, unusual for this period, is contemporaneous with the deposition of sand (Rieseberger Moor II, 1.33 - 1,41 m; samples 1.48 and 1.58 m of the new profile) and must be considered a local phenomenon. Literature: Schneekloth, Heinrich & Schneider, Siegfried (1971). Die Moore in Niedersachsen. 2. Teil. Bereich des Blattes Braunschweig der Geologischen Karte der Bundesrepublik Deutschland (1:200000). - Schriften der wirtschaftswissenschaftlichen Gesellschaft zum Studium Niedersachsens e.V. Reihe A I., Band 96, Heft 2, 83 Seiten, Göttingen. Selle, Willi (1935) Das Torfmoor bei Rieseberg. - Jahresbericht des Vereins für Naturwissenschaft zu Braunschweig, 23, 46-58, Braunschweig.