Isotopic composition of water in fog, rain, throughfall and stemflow in the Monte Verde, Costa Rica

Fog deposition, precipitation, throughfall and stemflow were measured in a windward tropical montane cloud forest near Monteverde, Costa Rica, for a 65-day period during the dry season of 2003. Net fog deposition was measured directly using the eddy covariance (EC) method and it amounted to 1.2 ± 0.1 mm/day (mean ± standard error). Fog water deposition was 5-9% of incident rainfall for the entire period, which is at the low end of previously reported values. Stable isotope concentrations (d18O and d2H) were determined in a large number of samples of each water component. Mass balance-based estimates of fog deposition were 1.0 ± 0.3 and 5.0 ± 2.7 mm/day (mean ± SE) when d18O and d2H were used as tracer, respectively. Comparisons between direct fog deposition measurements and the results of the mass balance model using d18O as a tracer indicated that the latter might be a good tool to estimate fog deposition in the absence of direct measurement under many (but not all) conditions. At 506 mm, measured water inputs over the 65 days (fog plus rain) fell short by 46 mm compared to the canopy output of 552 mm (throughfall, stemflow and interception evaporation). This discrepancy is attributed to the underestimation of rainfall during conditions of high wind.

Soil, snow, weather, and sub-surface storage data from a mountain catchment in the rain-snow transition zone

A comprehensive hydroclimatic data set is presented for the 2011 water year to improve understanding of hydrologic processes in the rain-snow transition zone. This type of dataset is extremely rare in scientific literature because of the quality and quantity of soil depth, soil texture, soil moisture, and soil temperature data. Standard meteorological and snow cover data for the entire 2011 water year are included, which include several rain-on-snow events. Surface soil textures and soil depths from 57 points are presented as well as soil texture profiles from 14 points. Meteorological data include continuous hourly shielded, unshielded, and wind corrected precipitation, wind speed, air temperature, relative humidity, dew point temperature, and incoming solar and thermal radiation data. Sub-surface data included are hourly soil moisture data from multiple depths from 7 soil profiles within the catchment, and soil temperatures from multiple depths from 2 soil profiles. Hydrologic response data include hourly stream discharge from the catchment outlet weir, continuous snow depths from one location, intermittent snow depths from 5 locations, and snow depth and density data from ten weekly snow surveys. Though it represents only a single water year, the presentation of both above and below ground hydrologic condition makes it one of the most detailed and complete hydro-climatic datasets from the climatically sensitive rain-snow transition zone for a wide range of modeling and descriptive studies.

Major ion concentrations in rain water and atmospheric moisture above the Atlantic Ocean

Concentratios of Cl-, Mg2+, Ca2+, and HCO3- ions were studied in rain waters and condensed atmospheric moisture above the Atlantic Ocean. Maximal number of samples was collected in the eastern tropical North Atlantic. Concentration of chloride ions ranged from 1 to 28 mg/l in rain waters (average 4.3 mg/l) and ranged from 0.3 to 2 mg/l in condensed atmospheric moisture with the average about one order of magnitude less than that for rain waters. Chloride normalized concentrations of magnesium and calcium are greater in rain waters and condensed atmospheric moisture than in ocean water due to more intensive subtraction of these ions as compared to chloride ions. Chloride normalized HCO3- concentration is one order of magnitude greater in atmospheric moisture than in seawater, possibly because of volatile component CO2 taking part in exchange between the ocean and the atmosphere.

Data compilation of hummingbird-pollinated plant species in the Brazilian Atlantic rain forest

We studied the relationship between flower size and nectar properties of hummingbird-visited flowers in the Brazilian Atlantic Forest. We analysed the nectar volume and concentration as a function of corolla length and the average bill size of visitors for 150 plant species, using the phylogenetic generalized least squares (PGLS) to control for phylogenetic signals in the data. We found that nectar volume is positively correlated with corolla length due to phylogenetic allometry. We also demonstrated that larger flowers provide better rewards for long-billed hummingbirds. Regardless of the causal mechanisms, our results support the hypothesis that morphological floral traits that drive partitioning among hummingbirds correspond to the quantity of resources produced by the flowers in the Atlantic Forest. We demonstrate that the relationship between nectar properties and flower size is affected by phylogenetic constraints and thus future studies assessing the interaction between floral traits need to control for phylogenetic signals in the data.

Lupane triterpenes from the leaves of the tropical rain forest tree Hopea odorata Roxb. and their cytotoxic activities, supplementary information

Chromatographic fractionation of the cytotoxic n-hexane extract of Hopea odorata Roxb. leaves led to the isolation of eight lupane triterpenes, which constitutes the first report of lupane-type triterpenes from this plant source. Furthermore, 3,30-dioxolup-20(29)-en-28-oic acid (6) was isolated for the first time from a natural source. Their structures were determined on the basis of spectroscopic methods, including 2D NMR analysis, and by comparison of their spectral data with literature values. Complete NMR assignments of the 1H and 13C NMR data were achieved for all compounds. Finally, the cytotoxic activities of the isolated compounds against four human cell lines (PC3, MDA-MB-231, HT-29 and HCT116) was also reported.