1 resultado para FREEZING
em University of Connecticut - USA
Filtro por publicador
- Abertay Research Collections - Abertay University’s repository (1)
- Academic Archive On-line (Stockholm University; Sweden) (1)
- Adam Mickiewicz University Repository (1)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (9)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (1)
- Aquatic Commons (85)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (13)
- Archimer: Archive de l'Institut francais de recherche pour l'exploitation de la mer (1)
- Archive of European Integration (6)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (3)
- Aston University Research Archive (21)
- B-Digital - Universidade Fernando Pessoa - Portugal (1)
- Biblioteca de Teses e Dissertações da USP (5)
- Biblioteca Digital | Sistema Integrado de Documentación | UNCuyo - UNCUYO. UNIVERSIDAD NACIONAL DE CUYO. (1)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (22)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (10)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (7)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (49)
- Boston University Digital Common (1)
- Brock University, Canada (2)
- Bucknell University Digital Commons - Pensilvania - USA (1)
- CaltechTHESIS (5)
- Cambridge University Engineering Department Publications Database (6)
- CentAUR: Central Archive University of Reading - UK (42)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (40)
- Cochin University of Science & Technology (CUSAT), India (10)
- Coffee Science - Universidade Federal de Lavras (2)
- Collection Of Biostatistics Research Archive (2)
- CORA - Cork Open Research Archive - University College Cork - Ireland (2)
- Dalarna University College Electronic Archive (1)
- Deakin Research Online - Australia (6)
- Digital Commons - Michigan Tech (4)
- Digital Commons - Montana Tech (2)
- Digital Commons @ DU | University of Denver Research (1)
- Digital Commons at Florida International University (2)
- DigitalCommons - The University of Maine Research (4)
- DigitalCommons@The Texas Medical Center (2)
- DigitalCommons@University of Nebraska - Lincoln (3)
- Diposit Digital de la UB - Universidade de Barcelona (1)
- Duke University (3)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (12)
- Greenwich Academic Literature Archive - UK (1)
- Harvard University (1)
- Helda - Digital Repository of University of Helsinki (7)
- Illinois Digital Environment for Access to Learning and Scholarship Repository (1)
- Indian Institute of Science - Bangalore - Índia (78)
- Infoteca EMBRAPA (1)
- Institutional Repository of Leibniz University Hannover (1)
- INSTITUTO DE PESQUISAS ENERGÉTICAS E NUCLEARES (IPEN) - Repositório Digital da Produção Técnico Científica - BibliotecaTerezine Arantes Ferra (1)
- Instituto Gulbenkian de Ciência (1)
- Instituto Politécnico de Bragança (1)
- Lume - Repositório Digital da Universidade Federal do Rio Grande do Sul (2)
- Memorial University Research Repository (1)
- National Center for Biotechnology Information - NCBI (20)
- Portal de Revistas Científicas Complutenses - Espanha (1)
- Publishing Network for Geoscientific & Environmental Data (49)
- QSpace: Queen's University - Canada (5)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (25)
- Queensland University of Technology - ePrints Archive (35)
- RCAAP - Repositório Científico de Acesso Aberto de Portugal (1)
- ReCiL - Repositório Científico Lusófona - Grupo Lusófona, Portugal (1)
- Repositório Aberto da Universidade Aberta de Portugal (1)
- Repositório Alice (Acesso Livre à Informação Científica da Embrapa / Repository Open Access to Scientific Information from Embrapa) (2)
- Repositório Científico do Instituto Politécnico de Lisboa - Portugal (2)
- REPOSITORIO DIGITAL IMARPE - INSTITUTO DEL MAR DEL PERÚ, Peru (1)
- Repositório Institucional da Universidade de Aveiro - Portugal (4)
- Repositório Institucional da Universidade de Brasília (1)
- Repositório Institucional da Universidade Estadual de São Paulo - UNESP (3)
- Repositório Institucional da Universidade Federal de São Paulo - UNIFESP (1)
- Repositório Institucional da Universidade Federal do Rio Grande - FURG (5)
- Repositório Institucional da Universidade Tecnológica Federal do Paraná (RIUT) (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (258)
- SAPIENTIA - Universidade do Algarve - Portugal (3)
- Universidad del Rosario, Colombia (2)
- Universidad Politécnica de Madrid (15)
- Universidade Complutense de Madrid (2)
- Universidade de Lisboa - Repositório Aberto (1)
- Universidade dos Açores - Portugal (2)
- Universidade Federal do Pará (8)
- Universidade Federal do Rio Grande do Norte (UFRN) (12)
- Universidade Técnica de Lisboa (2)
- Universitat de Girona, Spain (4)
- Université de Lausanne, Switzerland (1)
- Université de Montréal, Canada (4)
- University of Connecticut - USA (1)
- University of Michigan (15)
- University of Queensland eSpace - Australia (10)
Resumo:
Lack of linearity and sensitivity, oxygen dependence, biofouling and tissue inflammation hinder the development of implantable biosensors for continuous monitoring of glucose. Herein, we report the development of stacked outer membranes based on LBL/PVA hydrogels that improve sensor sensitivity, linearity, oxygen independence and counter biofouling and inflammation. While the inner LBL membrane affords tunable diffusivity, the outer PVA is capable of releasing anti-inflammatory drugs/tissue response modifying agents to counter acute and chronic inflammation, and to induce neo-angiogenesis at the implant site. Sensors were fabricated by immobilizing GOx enzyme on top of 50 μm platinum wires, followed by deposition of stacked LBL/PVA hydrogel membranes. The response of the sensors at 0.7V to various glucose concentrations was studied. Michelis-Menten analysis was performed to quantify sensor performance in terms of linearity and oxygen dependence. The interplay between sensor performance and inward glucose diffusivity was elucidated using (i) various LBL membranes and (ii) various freeze-thaw (FT) cycles of PVA. Incorporation of LBL/PVA stacked membranes resulted in an 8 fold increase in sensor linearity and a 9 fold decrease in oxygen dependence compared to controls. The enhancement in the sensor performance is attributed to (i) the oxygen storing capability of PVA hydrogel due to the formation of hydrophobic domains during its freezing/ thawing employed for its physical crosslinking and (ii) regulation of glucose flux by the inner LBL membrane. Such membranes offer significant advantages over presently available outer membranes in lieu of (i) their ability to control inflammation, (ii) their modulus that closely matches that of subcutaneous human tissue, (iii) non-necessity of reactive chemical crosslinking agents, (iv) tunable sensitivity and (v) supplemental storage of oxygen.