Micro-fabrication of bio-MEMS based force sensor to measure the force response of living cells

Understanding how a living cell behaves has become a very important topic in today’s research field. Hence, different sensors and testing devices have been designed to test the mechanical properties of these living cells. This thesis presents a method of micro-fabricating a bio-MEMS based force sensor which is used to measure the force response of living cells. Initially, the basic concepts of MEMS have been discussed and the different micro-fabrication techniques used to manufacture various MEMS devices have been described. There have been many MEMS based devices manufactured and employed for testing many nano-materials and bio-materials. Each of the MEMS based devices described in this thesis use a novel concept of testing the specimens. The different specimens tested are nano-tubes, nano-wires, thin film membranes and biological living cells. Hence, these different devices used for material testing and cell mechanics have been explained. The micro-fabrication techniques used to fabricate this force sensor has been described and the experiments preformed to successfully characterize each step in the fabrication have been explained. The fabrication of this force sensor is based on the facilities available at Michigan Technological University. There are some interesting and uncommon concepts in MEMS which have been observed during this fabrication. These concepts in MEMS which have been observed are shown in multiple SEM images.

ANALYSIS OF GENDER RELATIONS IN THE INDUSTRIAL COMMUNITY OF AGUIRRE, PUERTO RICO

This thesis is a study of the gender relations of the residents of Aguirre, Puerto Rico, between 1940 and 1991. The primary goal of the project was to explore how gender roles and relations in the Aguirre community were impacted by the social class system introduced by the Aguirre Sugar Company. This project was based on the interpretation of the past and present situation of the Aguirre community using oral history, by conducting a series of interviews among its residents. The interviews resulted in three main themes. First, the concepts of `normal and natural' were used to distinguish gender roles. Second, Aguirreños identified `family as community', since through the family individuals built their gender identity and learned the basic rules of coexistence within the social hierarchy of the community. Third, although the gender and class roles were clear in the community, `resistance and negotiation' occurred in the home and at the Company between those of different gender and social classes. The Aguirre Sugar Company was one of the principal influences on the construction of the Aguirreños identity, and left a mark on the past, present and future generations.