Suitability of real-time quantitative PCR to estimate the relative telomere length in European Hake (Merluccius merluccius Linnaeus, 1758)

Telomere length measurement has been proposed as a promising tool to estimate the age of individuals in natural populations. We used real-time quantitative PCR (qPCR) to measure relative telomere length in four tissues (brain, kidney, liver and muscle) of European hake (Merluccius merluccius) in different groups based upon body length an otolith age estimate. We observed a high level of inter-individual differences in the measurements of relative telomere length in hakes of similar age and body length groups. The results of qPCR analysis showed a great variability in all measures and a lack of repeatability and reproducibility with significant statistical differences in the results of the different assays. The paper discusses the technical reasons for the variability in qPCR obtained in this work and by other authors.

Tarnishing the Golden Stool

This thesis deals with the Asante federation (in modern-day Ghana ) in the nineteenth century, within the wider context of a general crisis of authority in African states in the later partof the century. African states were fighting against the external pressures of European imperial expansion while facing internal challenges to their structures of power. Asante was one of these states and this thesis examines why, because of a particular combination of internal and external challenges, Asante was unable to maintain its independence at the end of the nineteenth century.