So Very Far Away

I began my thesis planning to craft pieces of both fiction and nonfiction. I had very much enjoyed working on a travel essay during the fall semester, but I have always had an inherent interest in writing short stories, so I had hoped to explore both genres, using my previous work from a seminar in fiction and a travel writing class as a springboard. However, in writing “So Very Far Away,” my second nonfiction piece, which I developed from material that I was unableto keep in the essay “Lunch with the Americans,” I discovered that nonfiction was an incredibly freeing genre because the essential ingredients were already present. My personal experiences provided the basic elements of plot, character, and setting, yet I discovered new challenges as I attempted to present these experiences in a way that moved beyond mere memories and reflections and towards a larger meaning that would matter to others as well. I became sointrigued by this process of creating cohesive narratives through the editing and shaping of memories that I decided to focus completely on nonfiction for my thesis work and write a collection of essays about my time abroad.

Continuous Formation and Separation of Calcium-Alginate Capsules Containing Viable Mammalian Cells in Microfluidic Devices

Microfluidic devices can be used for many applications, including the formation of well-controlled emulsions. In this study, the capability to continuously create monodisperse droplets in a microfluidic device was used to form calcium-alginate capsules.Calcium-alginate capsules have many potential uses, such as immunoisolation of cells and microencapsulation of active drug ingredients or bitter agents in food or beverage products. The gelation of calcium-alginate capsules is achieved by crosslinking sodiumalginate with calcium ions. Calcium ions dissociated from calcium carbonate due to diffusion of acetic acid from a sunflower oil phase into an aqueous droplet containing sodium-alginate and calcium carbonate. After gelation, the capsules were separated from the continuous oil phase into an aqueous solution for use in biological applications. Typically, capsules are separated bycentrifugation, which can damage both the capsules and the encapsulated material. A passive method achieves separation without exposing the encapsulated material or the capsules to large mechanical forces, thereby preventing damage. To achieve passiveseparation, the use of a microfluidic device with opposing channel wa hydrophobicity was used to stabilize co-laminar flow of im of hydrophobicity is accomplished by defining one length of the channel with a hydrogel. The chosen hydrogel was poly (ethylene glycol) diacrylate, which adheres to the glass surface through the use of self-assembled monolayer of 3-(trichlorosilyl)-propyl methacrylate. Due to the difference in surface energy within the channel, the aqueous stream is stabilized near a hydrogel and the oil stream is stabilized near the thiolene based optical adhesive defining the opposing length of the channel. Passive separation with co-laminar flow has shown success in continuously separating calcium-alginatecapsules from an oil phase into an aqueous phase. In addition to successful formation and separation of calcium alginate capsules,encapsulation of Latex micro-beads and viable mammalian cells has been achieved. The viability of encapsulated mammalian cells was determined using a live/dead stain. The co-laminar flow device has also been demonstrated as a means of separating liquid-liquidemulsions.