Evaluating Bucknell's Waste Stream

This thesis examines three questions regarding the content of Bucknell University‟s waste stream and the contributors to campus recycling and solid waste disposal. The first asks, “What does Bucknell‟s waste stream consist of?” To answer this question, I designed a campus-wide waste audit procedure that sampled one dumpster from each of the eleven „activity‟ types on campus in order to better understand Bucknell‟s waste composition. The audit was implemented during the Fall semester of the 2011-2012 school year. The waste from each dumpster was sorted into several recyclable and non-recyclable categories and then weighed individually. Results showed the Bison and Carpenter Shop dumpsters to contain the highest percentage of divertible materials (through recycling and/or composting). When extrapolated, results also showed the Dining Services buildings and Facilities buildings to be the most waste dense in terms of pounds of waste generated per square foot. The Bison also generated the most overall waste by weight. The average composition of all dumpsters revealed that organic waste composed 24% of all waste, 23% was non-recyclable paper, and 20% was non-recyclable plastic. It will be important to move forward using these results to help create effective waste programs that target the appropriate areas of concern. My second question asks, “What influences waste behavior to contribute to this „picture‟ of the waste stream?” To answer this question, I created a survey that was sent out to randomly selected sub-group of the university‟s three constituencies: students, faculty, and staff. The survey sought responses regarding each constituency‟s solid waste disposal and recycling behavior, attitudes toward recycling, and motivating factors for solid waste disposal behaviors across different sectors of the university. Using regression analysis, I found three statistically significant motivating factors that influence solid waste disposal behavior: knowledge and awareness, moral value, and social norms. I further examined how a person‟s characteristics associate to these motivating factors and found that one‟s position on campus proved a significant association. Consistently, faculty and staff were strongly influenced by the aforementioned motivating factors, while students‟ behavior was less influenced by them. This suggests that new waste programs should target students to help increase the influence of these motivators to improve the recycling rate and lower overall solid waste disposal on campus. After making overall conclusions regarding the waste audit and survey, I ask my third question, which inquires, “What actions can Bucknell take to increase recycling rates and decrease solid waste generation?” Bucknell currently features several recycling and waste minimization programs on campus. However, using results from the waste audit and campus survey, we can better understand what are the issues of the waste stream, how do we go about addressing these issues, and who needs to be addressed. I propose several suggestions for projects that future students may take on for summer or thesis research. Suggestions include targeting the appropriate categories of waste that occur most frequently in the waste stream, as well as the building types that have the highest waste density and potential recovery rates. Additionally, certain groups on campus should be targeted more directly than others, namely the student body, which demonstrates the lowest influence by motivators of recycling and waste behavior. Several variables were identified as significant motivators of waste and recycling behavior, and could be used as program tactics to encourage more effective behavior.

The Effects of Altered Prenatal Melatonin Signaling on Adult Behavior and Hippocampal Gene Expression of the Male Rat: A Circadioneuroendocrine-Axis Hypothesis of Psychopathology

Disturbances in melatonin - the neurohormone that signals environmental darkness as part of the circadian circuit of mammals - have been implicated in various psychopathologies in humans. At present, experimental evidence linking prenatal melatonin signaling to adult physiology, behavior, and gene expression is lacking. We hypothesized that administration of melatonin (5 mg/kg) or the melatonin receptor antagonist luzindole (5 mg/kg) to rats in utero would permanently alter the circadian circuit to produce differential growth, adult behavior, and hippocampal gene expressionin the male rat. Prenatal treatment was found to increase growth in melatonin-treated animals. In addition, subjects exposed to melatonin prenatally displayed increased rearing in the open field test and an increased right turn preference in the elevated plusmaze. Rats administered luzindole prenatally, however, displayed greater freezing and grooming behavior in the open field test and improved learning in the Morris water maze. Analysis of relative adult hippocampal gene expression with RT-PCR revealed increasedexpression of brain-derived neurotrophic factor (BDNF) with a trend toward increased expression of melatonin 1A (MEL1A) receptors in melatonin-exposed animals whereas overall prenatal treatment had a significant effect on microtubule-associated protein 2(MAP2) expression. Our data support the conclusion that the manipulation of maternal melatonin levels alters brain development and leads to physiological and behavioral abnormalities in adult offspring. We designate the term circadioneuroendocrine (CNE)axis and propose the CNE-axis hypothesis of psychopathology.

The Effects of Apomorphine on Sexual Behavior and Aggression in Male Golden Hamsters

The general dopamine agonist apomorphine has been shown to have mostly facilitative effects on sexual behavior in rodents (Domingues & Hull, 2005; Bitran & Hull, 1987). A study looking at the effectsof apomorphine on sexual behavior in male golden hamsters observed that after systemic injections of apomorphine the males became aggressive towards the estrous females (Floody, unpublished). Studies on aggressive behavior have shown that apomorphine has facilitative effects on aggression in rodents (Nelson & Trainor, 2007; van Erp & Miczek, 2000; Ferrari, van Erp, Tornatzky, & Miczek, 2003). The studies presented here attempt to unravel the effects that apomorphine has on sexual and aggressive behavior in male golden hamsters. Studies 1, 2, 3, and 4 focused on the effects of apomorphine on aggression and Study 5 focused on the effects of apomorphine on sexual behavior. It was important for the purposes ofthis study to have separate, specific measures of aggression and sexual behavior that did not involve a social context that would involve multiple behaviors and motivations. The measure used to assessaggression was flank marking behavior. The measure used to assess sexual behavior was the number of vocalizations in response to sexual stimuli. The results from Studies 1, 2, and 3 suggested thatapomorphine increased aggressive motivation in a dose-dependent manner. In Studies 1 and 2 there was a high occurrence of stereotyped cheek pouching that interfered with the flank marking behavior. In Study 3 the procedure was modified to prevent cheek pouching and flank marking was observed uninhibited. Study 5 suggested a decrease in vocalizations after apomorphine treatment. However, this decrease may have been a result of the increase in stereotyped licking behavior. Results suggested that systemic apomorphine treatments increase aggressive motivation in hamsters. The increase in aggressive motivation may confuse the perception of the sensory signals that the males receive from the estrous females. They may haveperceived the estrous female as a nonestrous female which they would normally associate with an aggressive interaction (Lehman, Powers, & Winans, 1983).

The Behavior Rating Inventory Of Executive Function - Self-Report Version (Brief-Sr) And The Delis-Kaplan Executive Function Sys

The assessment of executive functions is an area of study that has seen considerable development in recent years. Despite much research examining the validity of various measures of executive functions from both a direct and indirect format, little evidence exists in the extant literature evaluating the correspondence between these types of measures. The current study examined the extent of correspondence, comprising concurrent validity, between the Delis-Kaplan Executive Function System (D-KEFS) and the Behavior Rating Inventory of Executive Function ¿ Self-Report Version (BRIEF-SR). Participants included 30 undergraduate and high school students 18 years of age. Results indicated mixed evidence of concurrent validity between the two measures of executive functions. The findings obtained suggest both expected significant, negative correlation as well as lack of expected correlation between the measures. Suggestions for future research in the assessment of executive functions are discussed.

The Effect of Chronic Estrogen Administration on Same-Sex Social Behavior and Central Oxytocin Levels in Prairie Voles (Microtus ochrogaster)

The purpose of our study is to investigate the effects of chronic estrogen administration on same-sex interactions during exposure to a social stressor and on oxytocin (OT) levels in prairie voles (Microtus orchrogaster). Estrogen and OT are two hormones known to be involved with social behavior and stress. Estogen is involved in the transcription of OT and its receptor. Because of this, it is generally thought that estrogen upregulates OT, but evidence to support this assumption is weak. While estrogen has been shown to either increase or decrease stress, OT has been shown to have stress-dampening properties. The goal of our experiment is to determine how estrogen affects OT levels as well as behavior in a social stressor in the voles. In addition, estrogen is required for many opposite-sex interactions, but little is known about its influence on same-sex interactions. We hypothesized that prairie voles receiving chronic estrogen injections would show an increase in OT levels in the brain and alter behavior in response to a social stressor called the resident-intruder test. To test this hypothesis, 73 female prairie voles were ovariectomized and then administered daily injections of estrogen (0.05 ¿g in peanut oil, s.c.) or vehicle for 8 days. On the final day of injections, half of the voles were given the resident-intruder test, a stressful 5 min interaction with a same-sex stranger. Their behavior was video-recorded. These animals were then sacrificed either 10 minutes or 60 minutes after the conclusion of the test. Half of the animals (no stress group) were not given the resident-intruder test. After sacrifice, trunk blood and brains were collected from the animals. Videos of the resident-intruder tests were analyzed for pro-social and aggressive behavior. Density of OT-activated neurons in the brain was measured via pixel count using immunohistochemistry. No differences were found in pro-social behavior (focal sniffing, p = 0.242; focal initiated sniffing p = 0.142; focal initiated sniffing/focal sniffing, p = 0.884) or aggressive behavior (total time fighting, p= 0.763; number of fights, p= 0.148; number of strikes, p = 0.714). No differences were found in activation of OT neurons in the brain, neither in the anterior paraventricular nucleus (PVN) (pixel count p= 0.358; % area that contains pixelated neurons p = 0.443) nor in the medial PVN (pixel count p= 0.999; % area that contains pixelated neurons p = 0.916). These results suggest that estrogen most likely does not directly upregulate OT and that estrogen does not alter behavior in stressful social interactions with a same-sex stranger. Estrogen may prepare the animal to respond to OT, instead of increasing the production of the peptide itself, suggesting that we need to shift the framework in which we consider estrogen and OT interactions.