Access to specialized pediatric cancer care in Switzerland

Specialized pediatric cancer centers (PCCs) are thought to be essential to obtain state-of-the-art care for children and adolescents. We determined the proportion of childhood cancer patients not treated in a PCC, and described their characteristics and place of treatment.

Extended-spectrum cephalosporin-resistant Escherichia coli in community, specialized outpatient clinic and hospital settings in Switzerland

OBJECTIVES Resistance to extended-spectrum cephalosporins (ESCs) in Escherichia coli can be due to the production of ESBLs, plasmid-mediated AmpCs (pAmpCs) or chromosomal AmpCs (cAmpCs). Information regarding type and prevalence of β-lactamases, clonal relations and plasmids associated with the bla genes for ESC-R E. coli (ESC-R-Ec) detected in Switzerland is lacking. Moreover, data focusing on patients referred to the specialized outpatient clinics (SOCs) are needed. METHODS We analysed 611 unique E. coli isolated during September-December 2011. ESC-R-Ec were studied with microarrays, PCR/DNA sequencing for blaESBLs, blapAmpCs, promoter region of blacAmpC, IS elements, plasmid incompatibility group, and also implementing transformation, aIEF, rep-PCR and MLST. RESULTS The highest resistance rates were observed in the SOCs, whereas those in the hospital and community were lower (e.g. quinolone resistance of 22.6%, 17.2% and 9.0%, respectively; P = 0.003 for SOCs versus community). The prevalence of ESC-R-Ec in the three settings was 5.3% (n = 11), 7.8% (n = 22) and 5.7% (n = 7), respectively. Thirty isolates produced CTX-M ESBLs (14 were CTX-M-15), 5 produced CMY-2 pAmpC and 5 hyper-expressed cAmpCs due to promoter mutations. Fourteen isolates were of sequence type 131 (ST131; 10 with CTX-M-15). blaCTX-M and blaCMY-2 were associated with an intact or truncated ISEcp1 and were mainly carried by IncF, IncFII and IncI1plasmids. CONCLUSIONS ST131 producing CTX-M-15 is the predominant clone. The prevalence of ESC-R-Ec (overall 6.5%) is low, but an unusual relatively high frequency of AmpC producers (25%) was noted. The presence of ESC-R-Ec in the SOCs and their potential ability to be exchanged between hospital and community should be taken into serious consideration.

Estilos en las redes sociales en línea: un estudio basado en corpora

Las nuevas tecnologías y el procesamiento digital han facilitado considerablemente la lingüística de corpus, por ejemplo Internet es una herramienta fácil y barata para recopilar corpus. Internet es cada vez más popular y más importante para la comunicación a causa de la enorme influencia de los nuevos medios y ha afectado la vida y la sociedad de muchas maneras y en parte, de manera fundamental. No sorprende por eso que la lengua y la comunicación misma se vean afectadas. Uno de los fenómenos más interesantes dentro de la comunicación mediada por ordenadores (CMC) son las redes sociales en línea, que en pocos años se han convertido en un medio de comunicación muy difundido y en expansión continua. Su estudio es particularmente interesante porque debido al desarrollo constante de la tecnología las redes sociales en línea no son una entidad estática, sino que cambian incesantemente, introduciéndose frecuentemente novedades para su uso. Estas novedades están condicionadas por el medio electrónico que a su vez influye decisivamente en el estilo de comunicación empleado en redes sociales como Facebook y Tuenti. Al ser un nuevo medio de interacción social, las redes sociales en línea producen un estilo de comunicación propio. El objetivo de análisis de mi tesis es cómo los usuarios de Facebook y Tuenti de la ciudad de Málaga crean este estilo mediante el uso de rasgos fónicos propios de la variedad andaluza y de qué manera la actitud lingüística de los usuarios influye en el uso de dichos rasgos fónicos. Este estudio se basa en un corpus elaborado a partir de enunciados de informantes en Facebook y Tuenti. Un corpus constituido por transcripciones amplias de grabaciones de hablantes malagueños me sirve de corpus de comparación. Otra herramienta metodológica empleada para recopilar datos será la encuesta: un tipo de encuesta estará destinada a captar las actitudes de los participantes frente a diversos rasgos del habla andaluza/malagueña y otro a examinar por qué la gente utiliza estos rasgos en Facebook y Tuenti. Este estudio se apoya en los resultados de un estudio piloto que muestran que los factores sociales y lingüísticos analizados funcionan de manera distinta en el habla real y virtual. Debido a estos usos diferentes podemos considerar la comunicación electrónica de Facebook y Tuenti como un estilo condicionado por el tipo de espacio virtual. Se trata de un estilo que sirve a los usuarios para crear significado social y para expresar sus identidades a partir de la lengua.

Trypanosomal TAC40 constitutes a novel subclass of mitochondrial -barrel proteins specialized in mitochondrial genome inheritance

Mitochondria cannot form de novo but require mechanisms allowing their inheritance to daughter cells. In contrast to most other eukaryotes Trypanosoma brucei has a single mitochondrion whose single-unit genome is physically connected to the flagellum. Here we identify a β-barrel mitochondrial outer membrane protein, termed tripartite attachment complex 40 (TAC40), that localizes to this connection. TAC40 is essential for mitochondrial DNA inheritance and belongs to the mitochondrial porin protein family. However, it is not specifically related to any of the three subclasses of mitochondrial porins represented by the metabolite transporter voltage-dependent anion channel (VDAC), the protein translocator of the outer membrane 40 (TOM40), or the fungi-specific MDM10, a component of the endoplasmic reticulum–mitochondria encounter structure (ERMES). MDM10 and TAC40 mediate cellular architecture and participate in transmembrane complexes that are essential for mitochondrial DNA inheritance. In yeast MDM10, in the context of the ERMES, is postulated to connect the mitochondrial genomes to actin filaments, whereas in trypanosomes TAC40 mediates the linkage of the mitochondrial DNA to the basal body of the flagellum. However, TAC40 does not colocalize with trypanosomal orthologs of ERMES components and, unlike MDM10, it regulates neither mitochondrial morphology nor the assembly of the protein translocase. TAC40 therefore defines a novel subclass of mitochondrial porins that is distinct from VDAC, TOM40, and MDM10. However, whereas the architecture of the TAC40-containing complex in trypanosomes and the MDM10-containing ERMES in yeast is very different, both are organized around a β-barrel protein of the mitochondrial porin family that mediates a DNA–cytoskeleton linkage that is essential for mitochondrial DNA inheritance.

Estilos en las redes sociales en línea: un estudio basado en corpora

Das Internet wird ein immer populäreres und wichtigeres Kommunikationsmittel, besonders die sogenannten Social-Networking-Sites. In dieser Studie wird untersucht wie die Social-Networking-Sites, Facebook und Tuenti, die Kommunikation beeinflussen. In einem Korpus von Usern aus Málaga, wurde der Gebrauch von nicht-Standard Merkmalen analysiert und mit dem in der gesprochenen Sprache verglichen. Aus diesem Vergleich lässt sich schließen, dass die untersuchten sozialen und linguistischen Faktoren in der virtuellen und der reellen Sprache unterschiedlich funktionieren. Aufgrund dieses unterschiedlichen Gebrauchs kann die elektronische Kommunikation Facebook und Tuenti’s als Stil betrachtet werden, welcher den Usern dazu dient, soziale Bedeutung zu kreieren und ihre sprachliche Identität auszudrücken.

Pangea: A Workbench for Statically Analyzing Multi-Language Software Corpora

Software corpora facilitate reproducibility of analyses, however, static analysis for an entire corpus still requires considerable effort, often duplicated unnecessarily by multiple users. Moreover, most corpora are designed for single languages increasing the effort for cross-language analysis. To address these aspects we propose Pangea, an infrastructure allowing fast development of static analyses on multi-language corpora. Pangea uses language-independent meta-models stored as object model snapshots that can be directly loaded into memory and queried without any parsing overhead. To reduce the effort of performing static analyses, Pangea provides out-of-the box support for: creating and refining analyses in a dedicated environment, deploying an analysis on an entire corpus, using a runner that supports parallel execution, and exporting results in various formats. In this tool demonstration we introduce Pangea and provide several usage scenarios that illustrate how it reduces the cost of analysis.

Unique Sm core structure of U7 snRNPs: assembly by a specialized SMN complex and the role of a new component, Lsm11, in histone RNA processing.

A set of seven Sm proteins assemble on the Sm-binding site of spliceosomal U snRNAs to form the ring-shaped Sm core. The U7 snRNP involved in histone RNA 3' processing contains a structurally similar but biochemically unique Sm core in which two of these proteins, Sm D1 and D2, are replaced by Lsm10 and by another as yet unknown component. Here we characterize this factor, termed Lsm11, as a novel Sm-like protein with apparently two distinct functions. In vitro studies suggest that its long N-terminal part mediates an important step in histone mRNA 3'-end cleavage, most likely by recruiting a zinc finger protein previously identified as a processing factor. In contrast, the C-terminal part, which comprises two Sm motifs interrupted by an unusually long spacer, is sufficient to assemble with U7, but not U1, snRNA. Assembly of this U7-specific Sm core depends on the noncanonical Sm-binding site of U7 snRNA. Moreover, it is facilitated by a specialized SMN complex that contains Lsm10 and Lsm11 but lacks Sm D1/D2. Thus, the U7-specific Lsm11 protein not only specifies the assembly of the U7 Sm core but also fulfills an important role in U7 snRNP-mediated histone mRNA processing.

Annotating the meaning of discourse connectives in multilingual corpora

Discourse connectives are lexical items indicating coherence relations between discourse segments. Even though many languages possess a whole range of connectives, important divergences exist cross-linguistically in the number of connectives that are used to express a given relation. For this reason, connectives are not easily paired with a univocal translation equivalent across languages. This paper is a first attempt to design a reliable method to annotate the meaning of discourse connectives cross-linguistically using corpus data. We present the methodological choices made to reach this aim and report three annotation experiments using the framework of the Penn Discourse Tree Bank.

How comparable are parallel corpora? Measuring the distribution of general vocabulary and connectives

In this paper, we question the homogeneity of a large parallel corpus by measuring the similarity between various sub-parts. We compare results obtained using a general measure of lexical similarity based on χ2 and by counting the number of discourse connectives. We argue that discourse connectives provide a more sensitive measure, revealing differences that are not visible with the general measure. We also provide evidence for the existence of specific characteristics defining translated texts as opposed to non-translated ones, due to a universal tendency for explicitation.