Chiasmatic Infiltration Secondary to Late Malignant Transformation of Retinoma

To report the lethal course of malignant transformation of retinoma in an adult.

Efficient transformation of Lactococcus lactis IL1403 and generation of knock-out mutants by homologous recombination

Lactococcus lactis IL1403 is a Gram-positive bacterium of great biotechnological interest for food grade applications. Its use is however hampered by the difficulty to efficiently transform this strain. We here describe a detailed, optimized electrotransformation protocol which yields a transformation efficiency of 10(6) cfu/microg of DNA with the two E. coli Gram-positive shuttle vectors pC3 and pVA838. The utility of the protocol was demonstrated by the generation of single- and double-knock-out mutants by homologous recombination.