The adsorption of biomolecules to multi-walled carbon nanotubes is influenced by both pulmonary surfactant lipids and surface chemistry

Background During production and processing of multi-walled carbon nanotubes (MWCNTs), they may be inhaled and may enter the pulmonary circulation. It is essential that interactions with involved body fluids like the pulmonary surfactant, the blood and others are investigated, particularly as these interactions could lead to coating of the tubes and may affect their chemical and physical characteristics. The aim of this study was to characterize the possible coatings of different functionalized MWCNTs in a cell free environment. Results To simulate the first contact in the lung, the tubes were coated with pulmonary surfactant and subsequently bound lipids were characterized. The further coating in the blood circulation was simulated by incubating the tubes in blood plasma. MWCNTs were amino (NH2)- and carboxyl (-COOH)-modified, in order to investigate the influence on the bound lipid and protein patterns. It was shown that surfactant lipids bind unspecifically to different functionalized MWCNTs, in contrast to the blood plasma proteins which showed characteristic binding patterns. Patterns of bound surfactant lipids were altered after a subsequent incubation in blood plasma. In addition, it was found that bound plasma protein patterns were altered when MWCNTs were previously coated with pulmonary surfactant. Conclusions A pulmonary surfactant coating and the functionalization of MWCNTs have both the potential to alter the MWCNTs blood plasma protein coating and to determine their properties and behaviour in biological systems.

A Global Climatology of Baroclinically Influenced Tropical Cyclogenesis*

Tropical cyclogenesis is generally considered to occur in regions devoid of baroclinic structures; however, an appreciable number of tropical cyclones (TCs) form in baroclinic environments each year. A global climatology of these baroclinically influenced TC developments is presented in this study. An objective classification strategy is developed that focuses on the characteristics of the environmental state rather than on properties of the vortex, thus allowing for a pointwise “development pathway” classification of reanalysis data. The resulting climatology shows that variability within basins arises primarily as a result of local surface thermal contrasts and the positions of time-mean features on the subtropical tropopause. The pathway analyses are sampled to generate a global climatology of 1948–2010 TC developments classified by baroclinic influence: nonbaroclinic (70%), low-level baroclinic (9%), trough induced (5%), weak tropical transition (11%), and strong tropical transition (5%). All basins other than the North Atlantic are dominated by nonbaroclinic events; however, there is extensive interbasin variability in secondary development pathways. Within each basin, subregions and time periods are identified in which the relative importance of the development pathways also differs. The efficiency of tropical cyclogenesis is found to be highly dependent on development pathway. The peak efficiency defined in the classification subspace straddles the nonbaroclinic/trough-induced boundary, suggesting that the optimal environment for TC development includes a baroclinic contribution from an upper-level disturbance. By assessing the global distribution of baroclinically influenced TC formations, this study identifies regions and pathways whose further study could yield improvements in our understanding of this important subset of TC developments.

Is the development of infectious keratoconjunctivitis in Alpine ibex and Alpine chamois influenced by topographic features?

Infectious keratoconjunctivitis (IKC) caused by Mycoplasma conjunctivae is a widespread ocular affection of free-ranging Caprinae in the Alpine arc. Along with host and pathogen characteristics, it has been hypothesized that environmental factors such as UV light are involved in the onset and course of the disease. This study aimed at evaluating the role of topographic features as predisposing or aggravating factors for IKC in Alpine chamois (Rupicapra rupicapra rupicapra) and Alpine ibex (Capra ibex ibex). Geospatial analysis was performed to assess the effect of aspect (northness) and elevation on the severity of the disease as well as on the mycoplasmal load in the eyes of affected animals, using data from 723 ibex and chamois (583 healthy animals, 105 IKC-affected animals, and 35 asymptomatic carriers of M. conjunctivae), all sampled in the Swiss Alps between 2008 and 2010. An influence of northness was not found, except that ibex with moderate and severe signs of IKC seem to prefer more north-oriented slopes than individuals without corneal lesions, possibly hinting at a sunlight sensitivity consequent to the disease. In contrast, results suggest that elevation influences the disease course in both ibex and chamois, which could be due to altitude-associated environmental conditions such as UV radiation, cold, and dryness. The results of this study support the hypothesis that environmental factors may play a role in the pathogenesis of IKC.