The COLOSS BEEBOOK Volume I, Standard methods for Apis mellifera research: Introduction

The COLOSS BEEBOOK is a practical manual compiling standard methods in all fields of research on the western honey bee, Apis mellifera. The COLOSS network was founded in 2008 as a consequence of the heavy and frequent losses of managed honey bee colonies experienced in many regions of the world (Neumann and Carreck, 2010). As many of the world’s honey bee research teams began to address the problem, it soon became obvious that a lack of standardized research methods was seriously hindering scientists’ ability to harmonize and compare the data on colony losses obtained internationally. In its second year of activity, during a COLOSS meeting held in Bern, Switzerland, the idea of a manual of standardized honey bee research methods emerged. The manual, to be called the COLOSS BEEBOOK, was inspired by publications with similar purposes for fruit fly research (Lindsley and Grell, 1968; Ashburner 1989; Roberts, 1998; Greenspan, 2004).

The COLOSS BEEBOOK Volume II, Standard methods for Apis mellifera pest and pathogen research: Introduction

The COLOSS BEEBOOK is a practical manual compiling standard methods in all fields of research on the western honey bee, Apis mellifera. The COLOSS network was founded in 2008 as a consequence of the heavy and frequent losses of managed honey bee colonies experienced in many regions of the world (Neumann and Carreck, 2010). As many of the world’s honey bee research teams began to address the problem, it soon became obvious that a lack of standardized research methods was seriously hindering scientists’ ability to harmonize and compare the data on colony losses obtained internationally. In its second year of activity, during a COLOSS meeting held in Bern, Switzerland, the idea of a manual of standardized honey bee research methods emerged. The manual, to be called the COLOSS BEEBOOK, was inspired by publications with similar purposes for fruit fly research (Lindsley and Grell, 1968; Ashburner, 1989; Roberts, 1998; Greenspan, 2004).

Standard methods for Nosema research

Methods are described for working with Nosema apis and Nosema ceranae in the field and in the laboratory. For fieldwork, different sampling methods are described to determine colony level infections at a given point in time, but also for following the temporal infection dynamics. Suggestions are made for how to standardise field trials for evaluating treatments and disease impact. The laboratory methods described include different means for determining colony level and individual bee infection levels and methods for species determination, including light microscopy, electron microscopy, and molecular methods (PCR). Suggestions are made for how to standardise cage trials, and different inoculation methods for infecting bees are described, including control methods for spore viability. A cell culture system for in vitro rearing of Nosema spp. is described. Finally, how to conduct different types of experiments are described, including infectious dose, dose effects, course of infection and longevity tests

Standard methods for maintaining adult Apis mellifera in cages under in vitro laboratory conditions

Adult honey bees are maintained in vitro in laboratory cages for a variety of purposes. For example, researchers may wish to perform experiments on honey bees caged individually or in groups to study aspects of parasitology, toxicology, or physiology under highly controlled conditions, or they may cage whole frames to obtain newly emerged workers of known age cohorts. Regardless of purpose, researchers must manage a number of variables, ranging from selection of study subjects (e.g. honey bee subspecies) to experimental environment (e.g. temperature and relative humidity). Although decisions made by researchers may not necessarily jeopardize the scientific rigour of an experiment, they may profoundly affect results, and may make comparisons with similar, but independent, studies difficult. Focusing primarily on workers, we provide recommendations for maintaining adults under in vitro laboratory conditions, whilst acknowledging gaps in our understanding that require further attention. We specifically describe how to properly obtain honey bees, and how to choose appropriate cages, incubator conditions, and food to obtain biologically relevant and comparable experimental results. Additionally, we provide broad recommendations for experimental design and statistical analyses of data that arises from experiments using caged honey bees. The ultimate goal of this, and of all COLOSS BEEBOOK papers, is not to stifle science with restrictions, but rather to provide researchers with the appropriate tools to generate comparable data that will build upon our current understanding of honey bees.

„Offene Grenzen“ oder „freiwillige Ghetttoisierung“? Aktuelle Kontroversen um die Sicherung der Kontinuität des Judentums

Die jüdische Gemeinschaft gilt generell als Musterbeispiel einer gut integrierten, religiösen Minderheit. Tatsächlich jedoch bewirken gerade die jüngsten gesellschaftlichen Entwicklungen − verstärkte Säkularisierung und Individualisierung verbunden mit steigenden Mischehenraten und einer Neudefinitionder Geschlechterrollen − eine Infragestellung der Kontinuität europäisch-jüdischer Existenz.Seit den 70iger Jahren des 20. Jahrhunderts bewegt sich die Mischehenrate fast überall in der Diaspora bei über 50%. Da die Weitergabe des Judentums religionsgesetzlich nur über die Mutter erfolgt,stellt der Umgang mit nichtjüdischen Familienmitgliedern einen hochsensiblen Bereich für die Gemeinschaft dar. Die soziale und religiöse Integration von nichtjüdischen Ehefrauen und vaterjüdischenKindern ist auf Grund einer nicht selten willkürlich erscheinenden Aufnahmepraxis ein häufig tabuisierter Aspekt des Gemeindelebens, der zu permanenten Spannungen führt. Konflikte bezüglich der Zugehörigkeitskriterien aber auch der religiösen Rolle der Frau führen zu Polarisierungs- und Pluralisierungstendenzen. Im Rahmen eines Projektes des NFP 58 wurden aktuelle innerjüdische Grenzziehungsdebatten im Kontext des Schweizer Judentums auch mit Methoden der Oral History festgehalten und analysiert. Die Auseinandersetzungen innerhalb der schweizerisch-jüdischen Gemeinschaft wurden zudem mit Entwicklungen in anderen Ländern der Diaspora und in Israel verglichen. Es ergab sich das Bild einer dynamischen und zugleich jedoch tief gespaltenen Religionsgemeinschaft, innerhalb der sich die verschiedenen Richtungen („liberal“ bis „ultra-orthodox“) die Verantwortung für eine zunehmende Schwächung und Spaltung des jüdischen Volkes zuweisen. Bibliographie Benbassa, Esther u. Jean-Christophe Attias. 2001. Les Juifs ont-t-ils un avenir? Paris. Lattés. Gerson, Daniel.2012. Ausbreitung und Bedeutung des Judentums in der Schweiz.in : Religionen in der Schweiz. Bulletin Schweizerische Akademie der Geistes- und Sozialwissenschaften, Nr 2.Bern. Gerson, Daniel.2011. Partizipation ohne Konversion? Grenzziehungsdebatten in neuen jüdischen Gemeinschaften der Schweiz,in: Chilufim. Zeitschrift für Jüdische Kulturgeschichte, Nr.11.Wien. Phoibos. Gerson, Daniel.2010. Gemeinschaftsbildung und «demokratischer» Antisemitismus: Das Entstehen eines Schweizer Judentums im Spannungsverhältnis von Akkulturation, Einwanderung und Ausgrenzung, in: Wyrwa, Ulrich (Hrsg.): Einspruch und Abwehr. Die Reaktion des europäischen Judentums und die Entstehung des Antisemitismus in Europa. Frankfurt am Main. Campus. Lambert, Nick.2008. Jews and Europe in the Twenty-First Century. London. Vallentine Mitchell. Picard, Jacques.2007. Judentum in der Schweiz: zwischen religiöser, kultureller und politischer Identität,in: Baumann, Martin u. Jörg Stolz (Hrsg.); Eine Schweiz - viele Religionen. Bielefeld. transcript. Wasserstein, Bernard.1996. Vanishing Diaspora. The Jews in Europa since 1945. New York.Harvard University Press.

Assessing traditional knowledge on forest uses to understand forest ecosystem dynamics

Forest ecosystems worldwide are heavily influenced by human activities. Information on these human activities are key for understanding ecosystem dynamics, especially as some of these human activities have long-term consequences, i.e. legacy effects. We assessed the diversity of forest uses across the Swiss Alps and the respective traditional forest-related knowledge (TFRK) by conducting 56 oral history interviews in five regions. As TFRK tends to be underrepresented in written records, oral history proves to be a very valuable approach. We classified the information gathered in 61 specific forest use practices, characterized by activity, product and use, in order to gain a comprehensive picture of the regional variability but also cross-regional ubiquity of certain forest uses. Based on these results we are able to name the ecologically most relevant forest uses which were most likely, or potentially occurring in Central European forests. We suggest a simple, systematic historical assessment of these relevant forest uses to be applied in all studies on forest ecosystems. This procedure will enable scientists to better evaluate to what degree human activities had an impact on forest ecosystem dynamics in their study area.

The COLOSS BEEBOOK Volume II: Standard methods for Apis mellifera pest and pathogen research

n recent years, declines of honey bee populations have received massive media attention worldwide, yet attempts to understand the causes have been hampered by a lack of standardisation of laboratory techniques. Published as a response to this, the COLOSS BEEBOOK is a unique collaborative venture involving 234 bee scientists from 34 countries, who have produced the definitive guide to how to carry out research on honey bees. It is hoped that these volumes will become the standards to be adopted by bee scientists worldwide. Volume II includes approximately 600 separate protocols dealing with the study of the pests and diseases of the honey bee, Apis mellifera. These cover epidemiology and surveying techniques, virus diseases, bacterial diseases such as European and American foulbrood, fungal and microsporidian diseases such as Nosema, mites such as Acarapis, Varroa and Tropilaelaps, and other pests such as the small hive beetle.

The COLOSS BEEBOOK Volume I: Standard Methods for Apis mellifera Research

In recent years, declines of honey bee populations have received massive media attention worldwide, yet attempts to understand the causes have been hampered by a lack of standardisation of laboratory techniques. Published as a response to this, the COLOSS BEEBOOK is a unique collaborative venture involving 234 bee scientists from 34 countries, who have produced the definitive guide to how to carry out research on honey bees. It is hoped that these volumes will become the standards to be adopted by bee scientists worldwide. Volume I includes approximately 1,100 separate protocols dealing with the study of the honey bee, Apis mellifera. These cover anatomy, behavioural studies, chemical ecology, breeding, genetics, instrumental insemination and queen rearing, pollination, molecular studies, statistics, toxicology and numerous other techniques