Moduli stabilisation and soft supersymmetry breaking in string compactifications

In this thesis, we shall work in the framework of type IIB Calabi-Yau flux compactifications and present a detailed review of moduli stabilisation studying in particular the phenomenological implications of the LARGE-volume scenario (LVS). All the physical relevant quantities such as moduli masses and soft-terms, are computed and compared to the phenomenological constraints that today guide the research. The structure of this thesis is the following. The first chapter introduces the reader to the fundamental concepts that are essentially supersymmetry-breaking, supergravity and string moduli, which represent the basic framework of our discussion. In the second chapter we focus our attention on the subject of moduli stabilisation. Starting from the structure of the supergravity scalar potential, we point out the main features of moduli dynamics, we analyse the KKLT and LARGE-volume scenario and we compute moduli masses and couplings to photons which play an important role in the early-universe evolution since they are strictly related to the decay rate of moduli particles. The third chapter is then dedicated to the calculation of soft-terms, which arise dynamically from gravitational interactions when moduli acquire a non-zero vacuum expectation value (VeV). In the last chapter, finally, we summarize and discuss our results, underling their phenomenological aspects. Moreover, in the last section we analyse the implications of the outcomes for standard cosmology, with particular interest in the cosmological moduli problem.

Sea organisms as a source of collagen-derived polymers for skin tissue engineering applications

Advanced therapies combating acute and chronic skin wounds are likely to be brought about using our knowledge of regenerative medicine coupled with appropriately tissue engineered skin substitutes. At the present time, there are no models of an artificial skin that completely replicate normal uninjured skin and they are usually accompanied by fibrotic reactions that result in the production of a scar. Natural biopolymers such as collagen have been a lot investigated as potential source of biomaterial for skin replacement in Tissue Engineering. Collagens are the most abundant high molecular weight proteins in both invertebrate and vertebrate organisms, including mammals, and possess mainly a structural role in connective tissues. From this, they have been elected as one of the key biological materials in tissue regeneration approaches, as skin tissue engineering. In addition, industry is constantly searching for new natural sources of collagen and upgraded methodologies for their production. The most common sources are skin and bone from bovine and porcine origin. However, these last carry high risk of bovine spongiform encephalopathy or transmissible spongiform encephalopathy and immunogenic responses. On the other hand, the increase of jellyfish has led us to consider this marine organism as potential collagen source for tissue engineering applications. In the present study, novel form of acid and pepsin soluble collagen were extracted from dried Rhopilema hispidum jellyfish species in an effort to obtain an alternative and safer collagen. We studied different methods of collagen purification (tissues and experimental procedures). The best collagen yield was obtained using pepsin extraction method (34.16 mg collagen/g of tissue). The isolated collagen was characterized by SDS-polyacrylamide gel electrophoresis and circular dichroism spectroscopy.