Phytoplankton silica uptake under different artificial upwelling conditions in the Canary Islands.

Climate change is affecting pelagic ecosystems with repercussions on fish production. In particular, global change is increasing oceanic temperature and stratification with decrease in nutrient input in euphotic layer leading to a decline in primary production. The mesocosm-based project Ocean Art-Up, conducted in Gran Canaria, is aimed to increase fish production and to enhance carbon sequestration through an artificial upwelling system. Diatoms dominate the phytoplankton community in upwelling systems and they need to take up silicates to grow. The abundance and nutritional value of diatoms determine the fate of phytoplankton biomass with transport to the upper level of the pelagic food web or to the deeper layer of the ocean with potential carbon sequestration. Here, data about experiments performed in 2018 and 2019 are reported. The first mesocosm experiment investigated the differences between pulsed and continuous upwelling mode, while the second experiment was conducted with a gradient in Si:N ratio along the mesocosms. The phytoplankton community takes up and incorporate silica about at the same rate in continuous mode, while in pulsed mode its peak occurred only after the deep-water addition. The diatom silica content is not affected by mode and amount of water added but by the Si:N ratio. Diatoms grown in an environment with high Si:N ratio values show higher abundance, biogenic silica production, silica uptake and silica content than the ones that experienced low Si:N values. In addition from literature, euphotic zone rich in silicate may produce high silica containing-diatoms who will produce repercussions on copepods community regarding feeding, hatching and growth, thus continuous upwelling with high Si:N ratio favours diatoms who will tend to sink and to be converted by copepods into fecal pellet rich in silica with increasing in potential carbon sequestration. Fish production may increase with continuous artificial upwelling showing low Si:N values.

Analysis and development of interface components and procedures for the design and commissioning toolchains in automation systems

The objective of the thesis project, developed within the Line Control & Software Engineering team of G.D company, is to analyze and identify the appropriate tool to automate the HW configuration process using Beckhoff technologies by importing data from an ECAD tool. This would save a great deal of time, since the I/O topology created as part of the electrical planning is presently imported manually in the related SW project of the machine. Moreover, a manual import is more error-prone because of human mistake than an automatic configuration tool. First, an introduction about TwinCAT 3, EtherCAT and Automation Interface is provided; then, it is analyzed the official Beckhoff tool, XCAD Interface, and the requirements on the electrical planning to use it: the interface is realized by means of the AutomationML format. Finally, due to some limitations observed, the design and implementation of a company internal tool is performed. Tests and validation of the tool are performed on a sample production line of the company.

A framework for the environmental risk assessment of offshore carbon storage in CCS systems

Carbon capture and storage (CCS) represents an interesting climate mitigation option, however, as for any other human activity, there is the impelling need to assess and manage the associated risks. This study specifically addresses the marine environmental risk posed by CO2 leakages associated to CCS subsea engineering system, meant as offshore pipelines and injection / plugged and abandoned wells. The aim of this thesis work is to start approaching the development of a complete and standardized practical procedure to perform a quantified environmental risk assessment for CCS, with reference to the specific activities mentioned above. Such an effort would be of extreme relevance not only for companies willing to implement CCS, as a methodological guidance, but also, by uniformizing the ERA procedure, to begin changing people’s perception about CCS, that happens to be often discredited due to the evident lack of comprehensive and systematic methods to assess the impacts on the marine environment. The backbone structure of the framework developed consists on the integration of ERA’s main steps and those belonging to the quantified risk assessment (QRA), in the aim of quantitatively characterizing risk and describing it as a combination of magnitude of the consequences and their frequency. The framework developed by this work is, however, at a high level, as not every single aspect has been dealt with in the required detail. Thus, several alternative options are presented to be considered for use depending on the situation. Further specific studies should address their accuracy and efficiency and solve the knowledge gaps emerged, in order to establish and validate a final and complete procedure. Regardless of the knowledge gaps and uncertainties, that surely need to be addressed, this preliminary framework already finds some relevance in on field applications, as a non-stringent guidance to perform CCS ERA, and it constitutes the foundation of the final framework.

Keyword-based multimedia data lookup in decentralized systems

I sistemi decentralizzati hanno permesso agli utenti di condividere informazioni senza la presenza di un intermediario centralizzato che possiede la sovranità sui dati scambiati, rischi di sicurezza e la possibilità di colli di bottiglia. Tuttavia, sono rari i sistemi pratici per il recupero delle informazioni salvate su di essi che non includano una componente centralizzata. In questo lavoro di tesi viene presentato lo sviluppo di un'applicazione il cui scopo è quello di consentire agli utenti di caricare immagini in un'architettura totalmente decentralizzata, grazie ai Decentralized File Storage e alla successiva ricerca e recupero di tali oggetti attraverso una Distributed Hash Table (DHT) in cui sono memorizzati i necessari Content IDentifiers (CID).\\ L'obiettivo principale è stato quello di trovare una migliore allocazione delle immagini all'interno del DHT attraverso l'uso dell'International Standard Content Code (ISCC), ovvero uno standard ISO che, attraverso funzioni hash content-driven, locality-sensitive e similarity-preserving, assegna i CID IPFS delle immagini ai nodi del DHT in modo efficiente, per ridurre il più possibile i salti tra i nodi e recuperare immagini coerenti con la query eseguita. Verranno, poi, analizzati i risultati ottenuti dall'allocazione dei CID delle immagini nei nodi mettendo a confronto ISCC e hash crittografico SHA-256, per verificare se ISCC rappresenti meglio la somiglianza tra le immagini allocando le immagini simili in nodi vicini tra loro.

Preliminary identification of dehalogenation specificities exhibited by dehalorespiring bacteria from a marine microbial community

The ability of a previously PCB-enriched microbial culture from Venice Lagoon marine sediments to dechlorinate pentachlorophenol (PCP) and 2,3,5-trichlorophenol (2,3,5-TCP) was confirmed under anaerobic conditions in microcosms consisting of site water and sediment. Dechlorination activities against Aroclor 1254 PCB mixture were also confirmed as control. Pentachlorophenol was degraded to 2,4,6-TCP (75.92±0.85 mol%), 3,5-DCP (6.40±0.75 mol%), and phenol (15.40±0.87 mol%). From the distribution of the different dechlorination products accumulated in the PCP-spiked cultures over time, two dechlorination pathways for PCP were proposed: (i) PCP to 2,3,4,6-TeCP, then to 2,4,6-TCP through the removal of both meta double-flanked chlorine substituents (main pathway); (ii) alternately, PCP to 2,3,5,6-TeCP, 2,3,5-TCP, 3,5-DCP, then phenol, through the removal of the para double-flanked chlorine, followed by ortho single-flanked chlorines, and finally meta unflanked chlorines (minor pathway). Removal of meta double-flanked chlorines is thus preferred over all other substituents. 2,3,5-TCP, that completely lacks double-flanked chlorines, was degraded to 3,5-DCP through removal of the ortho single-flanked chlorine, with a 99.6% reduction in initial concentration of 2,3,5-TCP by week 14. 16S rRNA PCR-DGGE using Chloroflexi-specific primers revealed a different role of the two microorganisms VLD-1 and VLD-2, previously identified as dechlorinators in the Aroclor 1254 PCB-enriched community, in the dehalogenation of chlorophenols. VLD-1 was observed both in PCP- and TCP-dechlorinating communities, whereas VLD-2 only in TCP-dechlorinating communities. This indicates that VLD-1 and VLD-2 may both dechlorinate ortho single-flanked chlorines, but only VLD-1 is able to remove double-flanked meta or para chlorines.