In vivo and in vitro effects of genistein as obesogenic/anti-obesogenic compound in the lipid metabolism of rainbow trout (Oncorhynchus mykiss)

Nowadays, soy is one of the most used ingredients in the formulation of fish feed, due to the ample market supply, lower market price, high protein concentration and favorable amino acid composition. Nevertheless, soybean meal products are rich and primary diet source of phytoestrogens, as genistein, which may have a potential negative impact on growth, hormonal regulation and lipid metabolism in fish. The principal aim of this study was to better understand in vivo and in vitro genistein’s effects on lipid metabolism of rainbow trout. In adipose tissue it was showed an unclear role of genistein on lipid metabolism in rainbow trout, and in liver an anti-obesogenic effect, with an up-regulation of autophagy-related genes LC3b (in adipose tissue) and ATG4b (in liver and adipose tissue), a down-regulation of apoptosis-related genes CASP3 (in adipose tissue) and CASP8 (in liver). An increase of VTG mRNA levels in liver was also observed. Genistein partially exerted these effects via estrogen- receptor dependent mechanism. In white muscle, genistein seemed to promote lipid turnover, up-regulating lipogenic (FAS and LXR) and lipolytic (HSL, PPARα and PPARβ) genes. It seemed that genistein could exert its lipolytic role via autophagic way (up-regulation of ATG4b and ATG12l), not through an apoptotic pathway (down-regulation of CASP3). The effects of genistein on lipid-metabolism and apoptosis-related genes in trout muscle were not dose-dependent, only on autophagy-related genes ATG4B and ATG12l. Moreover, a partial estrogenic activity of this phytoestrogen was also seen. Through in vitro analysis (MTT and ORO assay), instead, it was observed an anti-obesogenic effect of genistein on rainbow trout adipocytes, and this effect was not mediated by ERs. Both in vivo and in vitro, genistein exerted its effects in a dose-dependent manner.

In vivo and in vitro effects of antioxidant compounds and PPAR γ modulators on rainbow trout oxidative stress and lipid metabolism.

The aquafeed use of raw plant materials, as protein and lipid sources, has been considered and approved as a sustainable alternative to fish products (fish meal and oils) because the current trend to use high-lipid diets has been shown to induce undesirable increase in fat depots or further physiological alterations, such as induction of oxidative stress. In the aquaculture perspective, the addition of natural substances with antioxidant properties is an emerging strategy for protecting biological systems and foodstuffs from oxidative damage. Among natural substances, hydroxytyrosol (HT) and caffeic acid (CA) have attracted considerable attention as food antioxidant additives and modulators of physiological and molecular pathways involved in energy metabolism and adiposity. The aim of this study was to evaluate the effects of CA and HT on lipid metabolism and oxidative stress of rainbow trout (Oncorhynchus mykiss). In vitro results showed the potential anti-obesogenic effects of the compounds CA and HT on the adipose tissue of the rainbow trout. To support these data, in vitro assays performed (MTT, ORO, immunofluorescence) resulted in accordance among them; only results from proliferating cell nuclear antigen (PCNA) assay were not significant. In vivo results showed a possible anti-obesogenic effect of CA in liver and HT in adipose tissue. Regarding oxidative stress, we could hypothesize a possible anti-oxidant role of CA in liver.

La pubblicità tra presenza LGBTQ+, stereotipi di genere ed inclusione. Le tendenze della rappresentazione queer all’interno degli spot televisivi americani

La pubblicità – quella televisiva passando poi a quella sul web e sui social – subisce numerose trasformazioni che investono soprattutto i metodi comunicativi con cui sviluppare la propria narrazione. Oggi, di fatto, il mondo della pubblicità non si limita alla sola sponsorizzazione di un brand e dei suoi prodotti, ma si cala sempre più nelle dinamiche sociali, civili ed economiche che caratterizzano il nostro quotidiano. La comunicazione commerciale, dunque, si prefigge di essere il più inclusiva possibile, di coinvolgere storie, situazioni e personaggi che, nella pubblicità del “passato”, sarebbero stati esclusi totalmente o rappresentati in modo stereotipato. Se risulta possibile affermare che, nel panorama mediale odierno, si stia rivolgendo maggiore attenzione alle tematiche LGBTQ+, alle lotte contro la discriminazione di genere e all’ingaggio di personaggi appartenenti alla comunità queer, è altrettanto vero affermare come sia ancora possibile ricadere in rappresentazioni stereotipate e strategie di marketing che si fingono inclusive, solo per cavalcare l’onda del successo di eventi quali il Pride. All’interno di questo elaborato si andrà, in primis, a tracciare la storia culturale della comunità LGBTQ+ e ad approfondire come sia evoluta la sua presenza nei diversi media audiovisivi – dal cinema alle serie tv fino alla pubblicità. Ci si concentrerà poi sugli stereotipi ancora ricorrenti in pubblicità televisiva, ossia quello di genere. L’obiettivo della tesi è verificare se sia giusto parlare di inclusione o se si tratti solo una mera strategia di marketing, ossia di rainbow-washing, quando i brand si avvalgono di immagini e di personaggi LGBTQ+ nei propri spot. Nel capitolo finale, attraverso di un’analisi qualitativa di n. 100 spot televisivi americani, si andranno ad evidenziare i trend LGBTQ+, ossia in che modo oggi le pubblicità statunitensi sviluppano immagini, simboli, personaggi e storie LGBTQ+, realmente positive ed inclusive, in pubblicità.