Gene expression responses to acute and chronic heat stress in the common reef-building coral Pocillopora verrucosa

Global climate change is impacting coral reefs worldwide, with approximately 19% of reefs being permanently degraded, 15% showing symptoms of imminent collapse, and 20% at risk of becoming critically affected in the next few decades. This alarming level of reef degradation is mainly due to an increase in frequency and intensity of natural and anthropogenic disturbances. Recent evidence has called into question whether corals have the capacity to acclimatize or adapt to climate changes and some groups of corals showed inherent physiological tolerance to environmental stressors. The aim of the present study was to evaluate mRNA expression patterns underlying differences in thermal tolerance in specimen of the common reef-building coral Pocillopora verrucosa collected at different locations in Bangka Island waters (North Sulawesi, Indonesia). Part of the experimental work was carried out at the CoralEye Reef Research Outpost (Bangka Island). This includes sampling of corals at selected sites and at different depths (3 and 12 m) as well as their experimental exposure to an increased water temperature under controlled conditions for 3 and 7 days. Levels of mRNAs encoding ATP synthase (ATPs) NADH dehydrogenase (NDH) and a 70kDa Heat Shock Protein (HSP70) were evaluated by quantitative real time PCR. Transcriptional profiles evaluated under field conditions suggested an adaptation to peculiar local environmental conditions in corals collected at different sites and at the low depth. Nevertheless, high–depth collected corals showed a less pronounced site-to-site separation suggesting more homogenous environmental conditions. Exposure to an elevated temperature under controlled conditions pointed out that corals adapted to the high depth are more sensitive to the effects of thermal stress, so that reacted to thermal challenge by significantly over-expressing the selected gene products. Being continuously exposed to fluctuating environmental conditions, low-depth adapted corals are more resilient to the stress stimulus, and indeed showed unaffected or down-regulated mRNA expression profiles. Overall these results highlight that transcriptional profiles of selected genes involved in cellular stress response are modulated by natural seasonal temperature changes in P. verrucosa. Moreover, specimens living in more variable habitats (low-depth) exhibit higher basal HSP70 mRNA levels, possibly enhancing physiological tolerance to environmental stressors.

A maximum entropy approach to disturbed ecosystems

The current climate crisis requires a comprehensive understanding of biodiversity to acknowledge how ecosystems’ responses to anthropogenic disturbances may result in feedback that can either mitigate or exacerbate global warming. Although ecosystems are dynamic and macroecological patterns change drastically in response to disturbance, dynamic macroecology has received insufficient attention and theoretical formalisation. In this context, the maximum entropy principle (MaxEnt) could provide an effective inference procedure to study ecosystems. Since the improper usage of entropy outside its scope often leads to misconceptions, the opening chapter will clarify its meaning by following its evolution from classical thermodynamics to information theory. The second chapter introduces the study of ecosystems from a physicist’s viewpoint. In particular, the MaxEnt Theory of Ecology (METE) will be the cornerstone of the discussion. METE predicts the shapes of macroecological metrics in relatively static ecosystems using constraints imposed by static state variables. However, in disturbed ecosystems with macroscale state variables that change rapidly over time, its predictions tend to fail. In the final chapter, DynaMETE is therefore presented as an extension of METE from static to dynamic. By predicting how macroecological patterns are likely to change in response to perturbations, DynaMETE can contribute to a better understanding of disturbed ecosystems’ fate and the improvement of conservation and management of carbon sinks, like forests. Targeted strategies in ecosystem management are now indispensable to enhance the interdependence of human well-being and the health of ecosystems, thus avoiding climate change tipping points.