Food safety in wine: optimization of analytical controls and evaluation of production technologies

This PhD thesis has been proposed to validate and then apply innovative analytical methodologies for the determination of compounds with harmful impact on human health, such as biogenic amines and ochratoxin A in wines. Therefore, the influence of production technology (pH, amino acids precursor and use of different malolactic starters) on biogenic amines content in wines was evaluated. An HPLC method for simultaneous determination of amino acids and amines with precolumnderivatization with 9-Fluorenyl-methoxycarbonyl chloride (FMOC-Cl) and UV detection was developed. Initially, the influence of pH, time of derivatization, gradient profile were studied. In order to improve the separation of amino acids and amines and reduce the time of analysis, it was decided to study the influence of different flows and the use of different columns in the chromatographic method. Firstly, a C18 Luna column was used and later two monolithic columns Chromolith in series. It appeared to be suitable for an easy, precise and accurate determination of a relatively large number of amino acids and amines in wines. This method was then applied on different wines produced in the Emilia Romagna region. The investigation permitted to discriminate between red and white wines. Amino acids content is related to the winemaking process. Biogenic amines content in these wines does not represent a possible toxicological problem for human health. The results of the study of influence of technologies and wine composition demonstrated that pH of wines and amino acids content are the most important factors. Particularly wines with pH > 3,5 show higher concentration of biogenic amines than wines with lower pH. The enrichment of wines by nutrients also influences the content of some biogenic amines that are higher in wines added with amino acids precursors. In this study, amino acids and biogenic amines are not statistically affected by strain of lactic acid bacteria inoculated as a starter for malolactic fermentation. An evaluation of different clean-up (SPE-MycoSep; IACs and LLE) and determination methods (HPLC and ELISA) of ochratoxin A was carried out. The results obtained proved that the SPE clean-up are reliable at the same level while the LLE procedures shows lowest recovery. The ELISA method gave a lower determination and a low reproducibility than HPLC method.

La trasformazione dei processi legislativi nella società dell'informazione: dall'e-Legislation all'e-Parliament

The transformation of legislative processes in the Information society: from eLegislation to eParliament This research analyzes, by means of an interdisciplinary and comparative approach, the transformation of legislative processes produced by the introduction of new ICT technologies. The use of ICT in support of parliamentary activities is concerned with efficiency of parliamentary process and aims at more transparent procedures, improved access to documents, social participation and cooperation among institutions. With ICT Parliaments are now able to improve their efficiency and optimize their business; they can advance the dialogue with their citizen both, through the real access and the effective availability of information and, through new way of participation in the democratic process. Finally, sharing information, know-out, best practices and other records, Parliaments will be able to develop new information and knowledge and to strengthen the role and power of Institutions. Only through a global vision of the full process, re-thinking and develop rules and uniform standard and so implementing the new opportunities carrying out by ICT, it will be possible to put in practice concrete eParliament results. The Research goals are at least three: 1. To Analysed the legislative process and the ICT opportunities to understand the impact of the latter on the former. In particular to check up the problems that ICT can raise in relation of the constitutional principles ensuring the process itself. 2. To realized an abstract model representing the legislative process regardless of the form of government, chambers composition, legal system, etc. 3. To suggest standard, structural, linguistic and ontological, able to implement the new opportunities of sharing, cooperation and reuse among the many and various stakeholders of the democratic/legislative view.