CMoS lab-on-a-chip devices for individual cell biology

The development of microlectronic lab-on-a-chip devices (LOACs) can now be pursued thanks to the continous advances in silicon technology. LOACs are miniaturized devices whose aim is to perform in a more efficient way specific chemical or biological analysis protocols which are usually carried out with traditional laboratory equipment. In this application area, CMOS technology has the potential to integrate LOAC functionalities for cell biology applications in single chips, e.g. sensors, actuators, signal conditioning and processing circuits. In this work, after a review of the state of the art, the development of a CMOS prototype chip for individual cell manipulation and detection based on dielectrophoresis will be presented. Issues related to the embedded optical and capacitive detection of cells will be discussed together with the main experimental results obtained in manipulation and detection of living cells and microparticles.

Cosmic-Lab: Optical companions to binary Millisecond Pulsars

Millisecond Pulsars (MSPs) are fast rotating, highly magnetized neutron stars. According to the "canonical recycling scenario", MSPs form in binary systems containing a neutron star which is spun up through mass accretion from the evolving companion. Therefore, the final stage consists of a binary made of a MSP and the core of the deeply peeled companion. In the last years, however an increasing number of systems deviating from these expectations has been discovered, thus strongly indicating that our understanding of MSPs is far to be complete. The identification of the optical companions to binary MSPs is crucial to constrain the formation and evolution of these objects. In dense environments such as Globular Clusters (GCs), it also allows us to get insights on the cluster internal dynamics. By using deep photometric data, acquired both from space and ground-based telescopes, we identified 5 new companions to MSPs. Three of them being located in GCs and two in the Galactic Field. The three new identifications in GCs increased by 50% the number of such objects known before this Thesis. They all are non-degenerate stars, at odds with the expectations of the "canonical recycling scenario". These results therefore suggest either that transitory phases should also be taken into account, or that dynamical processes, as exchange interactions, play a crucial role in the evolution of MSPs. We also performed a spectroscopic follow-up of the companion to PSRJ1740-5340A in the GC NGC 6397, confirming that it is a deeply peeled star descending from a ~0.8Msun progenitor. This nicely confirms the theoretical expectations about the formation and evolution of MSPs.

COSMIC-LAB: Terzan 5 as a fossil remnant of the Galactic bulge formation epoch

The formation and evolution of galaxy bulges is a greatly debated topic in modern astrophysics. An approach to address this issue is to look at the Galactic bulge, the closest to us. According to some theoretical models, our bulge built-up from the merger of substructures formed from the instability and fragmentation of a proto-disk in the early phases of Galactic evolution. We may have discovered the remnant of one of these substructures: the stellar system Terzan 5. Terzan 5 hosts two stellar populations with different iron abundances, thus suggesting it once was far more massive than today. Moreover, its peculiar chemistry resembles that observed only in the Galactic bulge. In this Thesis we perform a detailed photometric and spectroscopic analysis of this cluster to determine its formation and evolutionary histories. Form the photometric point of view we built a high-resolution differential reddening map in Terzan 5 direction and we measured relative proper motions to separate its member population from the contaminating field stars. This information represents the necessary work to measure the absolute ages of Terzan 5 populations via the Turn-off luminosity method. From the spectroscopic point of view we measured abundances for more than 600 stars belonging to Terzan 5 and its surroundings in order to build the largest field-decontaminated metallicity distribution for this system. We find that the metallicity distribution is extremely wide (more than 1 dex) and we discovered a third, metal-poor and alpha-enhanced population with average [Fe/H]=-0.8. The striking similarity between Terzan 5 and the bulge in terms of their chemical formation and evolution revealed by this Thesis suggests that Terzan 5 formed in situ with the bulge itself. In particular its metal-poor populations trace the early stages of the bulge formation, while its most metal-rich component contains crucial information on the bulge more recent evolution.

COSMIC-LAB: Unexpected Results from High-resolution Spectra of AGB Stars in Globular Clusters

We have used high-resolution spectra, acquired with UVES@ESO-VLT, to determine the chemical abundances of different samples of AGB and RGB stars in 4 Galactic globular clusters, namely 47Tuc, NGC3201, M22 and M62. For almost all the analyzed AGB stars we found a clear discrepancy between the iron abundance measured from neutral lines and that obtained from single ionized lines, while this discrepancy is not obtained for the RGB samples observed in the same clusters and analyzed with the same procedure. Such a behavior exactly corresponds to what expected in the case of Non-Local Thermodynamical Equilibrium (NLTE) in the star atmosphere. These results have a huge impact on the proper determination of GC chemistry. In fact, one of the most intriguing consequences is that, at odds with previous claims, no iron spread is found in NGC3201 and M22 if the iron abundance is obtained from ionized lines only.